Abstract
By means of gas chromatography-selected ion monitoring-mass spectrometry using an isotope-dilution assay with 4,5,6,7-tetradeutero-indole-3-acetic acid as the internal standard, indole-3-acetic acid has been estimated to be present in aseptically cultured gametophytes of wild-type Physcomitrella patens (Hedw.) B.S.G. at a level of 0.075 μg g−1 dry weight or 2.1 ng g−1 fresh weight.
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Abbreviations
- IAA:
-
indole-3-acetic acid
- d4IAA:
-
4,5,6,7-tetra-deutero-indole-3-acetic acid
- [14C]IAA:
-
indole-3-[2-14C]-acetic acid
- GC-SIM-MS:
-
gas chromatography-selected ion monitoring-mass spectrometry
References
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Ashton, N.W., Schulze, A., Hall, P. et al. Estimation of indole-3-acetic acid in gametophytes of the moss, Physcomitrella patens . Planta 164, 142–144 (1985). https://doi.org/10.1007/BF00391040
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DOI: https://doi.org/10.1007/BF00391040