Abstract
Naphthylphthalamic acid (NPA), an inhibitor of polar auxin transport, binds with high affinity to membrane preparations from callus and cell suspension cultures derived from Nicotiana tabacum (K d approx. 2·10−9 M). The concentration of membrane-bound binding sites is higher in cell suspension than in callus cultures. The binding of NPA to these sites seems to be a simple process, in contrast to the binding of the synthetic auxin naphthylacetic acid (1-NAA) to membrane preparations from callus cultures, which is more complex (A.C. Maan et al., 1983, Planta 158, 10–15). Naphthylacetic acid, a number of structurally related compounds and the auxin-transport inhibitor triiodobenzoic acid were all able to compete with NPA for the same binding site with K d values ranging from 10−6 to 10−4 M. On the other hand, NPA was not able to displace detectable amounts of NAA from the NAA-binding site. A possible explantation is the existence of two different membrane-bound binding sites, one exclusively for auxins and one for NPA as well as auxins, that differ in concentration. The NPA-binding site is probably an auxin carrier.
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Abbreviations
- 1-NAA:
-
1-Naphthylacetic acid
- 2-NAA:
-
2-Naphthylacetic acid
- NPA:
-
N-1-Naphthylphthalamic acid
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Maan, A.C., Kühnel, B., Beukers, J.J.B. et al. Naphthylphthalamic acid-binding sites in cultured cells from Nicotiana tabacum . Planta 164, 69–74 (1985). https://doi.org/10.1007/BF00391027
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DOI: https://doi.org/10.1007/BF00391027