Abstract
Three peroxisomal enzymes, glycolate oxidase, urate oxidase and catalase were localized cytochemically in Psychotria punctata (Rubiaceae) leaves and Yucca torreyi (Agavaceae) seedling root tips, both of which contain developing and mature calcium-oxalate raphide crystal idioblasts. Glycolate-oxidase (EC 1.1.3.1) and catalase (EC 1.11.1.6) activities were present within leaftype peroxisomes in nonidioblastic mesophyll cells in Psychotria leaves, while urate-oxidase (EC 1.7.3.3) activity could not be conclusively demonstrated in these organelles. Unspecialized peroxisomes in cortical parenchyma of Yucca roots exhibited activities of all three enzymes. Reactionproduct deposits attributable to glycolate-oxidase activity were never observed in peroxisomes of any developing or mature crystal idioblasts of Psychotria or Yucca. Catalase localization indicates that idioblast microbodies are functional peroxisomes. The apparent absence of glycolate oxidase in crystal idioblasts of Psychotria and Yucca casts serious doubt that pathways involving this enzyme are operational in the synthesis of the oxalic acid precipitated as calcium-oxalate crystals in these cells.
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Abbreviations
- AMPD:
-
2-amino-2-methyl-1,3-propandiol
- CTEM:
-
conventional transmission electron microscopy
- DAB:
-
3,3′-diaminobenzidine tetrahydrochloride
- HVEM:
-
high-voltage electron microscopy
References
Armentrout, V.N., Graves, L.B., Jr., Maxwell, D.P. (1978) Localization of enzymes of oxalate biosynthesis in microbodies of Sclerotium rolfsii. Phytopathology 68, 1597–1599
Arnott, H.J. (1966) Studies of calcification in plants. IIIrd Eur. Symp. on Calcified Tissues, pp. 152–157, Blackwood, J.H., Owen, M., eds. Springer, Berlin Heidelberg New York
Arnott, H.J. (1973) Plant calcification. In: Biological mineralization, pp. 609–627, Zipkin, I., ed. Wiley, New York
Arnott, H.J., Pautard, F.G.E. (1970) Calcification in plants. In: Biological calcification; cellular and molecular aspects, pp. 375–446, Schraer, H., ed. Appleton-Century-Crofts, New York
Beevers, H. (1979) Microbodies in higher plants. Annu. Rev. Plant Physiol. 30, 159–193
Betsche, T. (1981) L-Lactate dehydrogenase from leaves of higher plants. Kinetics and regulation of the enzyme from lettuce (Lactuca sativa L.). Biochem. J. 195, 615–622
Bornkamm, R. (1965) Die Rolle des Oxalates im Stoffwechsel höherer grüner Pflanzen. Untersuchungen über Lemna minor L. Flora 156, 139–171
Chang, C.C., Beevers, H. (1968) Biogenesis of oxalate in plant tissue. Plant Physiol. 34, 403–409
Czapek, F. (1921) Biochemie der Pflanzen, vol. 3. Fischer, Jena
Davies, D.D., Asker, H. (1983) Synthesis of oxalic acid by enzymes from lettuce leaves. Plant Physiol. 72, 134–138
Eilert, G.B. (1974) An ultrastructural study of the development of raphide crystal cells in the roots of Yucca torreyi. Ph. D. dissertation, University of Texas, Austin, Diss. Abstr. Int. 35: 05-B
Foster, A.S. (1956) Plant idioblasts: remarkable examples of cell specialization. Protoplasma 46, 184–193
Franceschi, V.R., Horner, H.T. (1980) Calcium oxalate crystals in plants. Bot. Rev. 46, 361–427
Frank, E., Jensen, W.A. (1970) On the formation of the pattern of crystal idioblasts in Canavalia ensiformis DC. IV. The fine structure of crystal cells. Planta 95, 202–217
Frederick, S.E., Newcomb, E.H. (1969) Cytochemical localization of catalase in leaf microbodies (peroxisomes). J. Cell Biol. 43, 343–353
Hodgkinson, A. (1977) Oxalic acid in biology and medicine. Academic Press, London New York
Horner, H.T., Jr., Wagner, B.L. (1980) The association of druse crystals with the developing stomium of Capsicum annuum (Solanaceae) anthers. Am. J. Bot. 67, 1347–1360
Horner, H.T., Jr., Whitmoyer, R.E. (1972) Raphide crystal cell development in leaves of Psychotria punctata (Rubiaceae). J. Cell Sci. 11, 339–355
Huang, A.H.C. (1982) Metabolism in plant peroxisomes. In: Recent advances in phytochemistry, pp. 85–123, Creasy, L.L., Hrazdina, G., eds. Plenum Press, New York London
Huang, A.H.C., Beevers, H. (1971) Isolation of microbodies from plant tissues. Plant Physiol. 48, 637–641
Kausch, A.P., Horner, H.T. (1982) A comparison of calcium oxalate crystals isolated from callus cultures and their explant sources. Scanning electron Microsc. 2, 211–223
Kausch, A.P., Horner, H.T. (1983a) The development of mucilaginous raphide crystal idioblasts in young leaves of Typha angustifolia L. (Typhaceae). Am. J. Bot. 70, 691–705
Kausch, A.P., Horner, H.T. (1983b) Development of syncytial raphide crystal idioblasts in the cortex of adventitious roots of Vanilla planifolia L. (Orchidaceae). Scanning Electron Microsc. 2, 893–903
Kausch, A.P., Wagner, B.L., Horner, H.T. (1983) Use of the cerium chloride technique and energy dispersive X-ray microanalysis in plant peroxisome identification. Protoplasma 118, 1–9
Kpodar, M.P., Piquemal, M., Calmés, J., Latche, J. (1978) Relations entre nutrition azotée et metabolism photorespiratoire chez une plante à oxalate, Fagopyrum esculentum M. Physiol. Vég. 16, 117–130
Mollenhauer, H.H., Larson, D.A. (1966) Development changes in raphide forming cells in Vanilla planifolia and Monstera deliciosa. J. Ultrastruct. Res. 16, 55–70
Soderstrom, T.R. (1962) The isocitric acid content of Crassulacean plants and a few succulent species from other families. Am. J. Bot. 49, 850–855
Thomas, J., Trelease, R.N. (1981) Cytochemical localization of glycolate oxidase in microbodies (glyoxysomes and peroxisomes) of higher plant tissues with the CeCl3 technique. Protoplasma 108, 39–53
Thomas, R.J., Schrader, L.E. (1981) Ureide metabolism in higher plants. Phytochemistry 20, 361–371
Tolbert, N.E. (1973) Glycolate biosynthesis. In: Current topics of cellular regulation, pp. 21–50, Horecker, B.L., Stadman, E.R., eds. Academic Press, New York London
Tolbert, N.E. (1980) Microbodies — peroxisomes and glyoxysomes. In: The biochemistry of plants, vol. 1, pp. 359–388, Tolbert, N.E., ed. Academic Press, New York London
Tolbert, N.E., Essner, E. (1981) Microbodies: peroxisomes and glyoxysomes. J. Cell Biol. 91, 271S-283S
Vaughn, K.C., Duke, S.O., Hanson, C.A. (1982) Ultrastructural localization of urate oxidase in nodules of Sesbania exalta, Glycine max, and Medicago sativa. Histochemistry 74, 309–318
Vigil, E.L. (1970) Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J. Cell Biol. 46, 435–454
Wagner, G., Loewus, F. (1973) The biosynthesis of (+)-tartaric acid in Pelargonium crispum. Plant Physiol. 52, 651–654
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Kausch, A.P., Horner, H.T. Absence of CeCl3-detectable peroxisomal glycolate-oxidase activity in developing raphide crystal idioblasts in leaves of Psychotria punctata Vatke and roots of Yucca torreyi L.. Planta 164, 35–43 (1985). https://doi.org/10.1007/BF00391023
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DOI: https://doi.org/10.1007/BF00391023