Summary
Viable spheroplasts of a marine dinoflagellate, Gonyaulax polyedra, have been prepared for the first time. This simple and rapid procedure results in a yield of over 95% intact spheroplasts. Utilizing this technique, many studies of the cell-wall-free form of this dinoflagellate are now possible.
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References
Biggins, J.: Protoplasts of algal cells. In: Methods in enzymology. Part A. Photosynthesis 23, 209–211. Ed.: A. San Pietro; New York: Academic Press 1971
Cocking, E.C.: Plant cell protoplasts—isolation and development. Ann. Rev. Plant Physiol. 23, 29–50 (1972)
Gaff, D.F., OKong 'O-Ogola, O.: The use of nonpermeating pigments for testing the survival of cells. J. exp. Bot. 22, 756–758 (1971)
Gibor, A.: Surviving cytoplasts in vitro. Proc. nat. Acad. Sci. (Wash.) 54, 1527–1531 (1965)
Guillard, R.R.L., Ryther, J.H.: Studies of marine planktonic diatoms I. Cyclosella nana Hustedt and Detonula confervacea (Cleve) Gran. Canad. J. Microbiol 8, 229–239 (1962)
Hamman, J.P., Seliger, H.H.: The mechanical triggering of bioluminescence in marine dinoflagellates: chemical basis. J. Cell Physiol. 80, 397–408 (1972)
Hemmingsen, B.B.: A mono-silicic acid stimulated adenosinetriphosphatase from protoplasts of the apochloritic diatom Nitzchia alba. Ph. D. dissertation. p. 119. Scripps Institution of Oceanography, La Jolla (1971)
Herman, E.M., Sweeney, B.M.: Circadian rhythm of chloroplast ultrastructure in Gonyaulax polyedra, concentric organization around a cluster of ribosomes. J. ultrastr. Res. 50, 347–354 (1975)
Kaback, H.R.: Bacterial membranes. In: Methods in in enzymology. 22, 99–120, Ed.: W.B. Jakoby, New York: Academic Press 1971.
Kelley, J.W.: Quantitative cytochemistry of acid mucopolysaccharides. pp. 489–505. In: Introduction to quantitative cytochemistry. Ed.: G.L. Wied, New York: Academic Press 1966
Loeblich III, A.R.: The Amphiesma of dinoflagellate cell covering. In: Proc. North American Paleontological Convention. Part G. pp. 867–929 (1969)
Morvin, H.H., Helmy, F.M.: An introduction to comparative, correlative histochemical principles. Chapter 4: Carbohydrates. pp. 55–64, Jena: Gistav Fischer 1974
Ruesink, A.W.: Protoplasts of plant cells. In: Methods in enzymology, Part A. Photosynthesis 23, 197–209. Ed.: A. San Pietro, New York: Academic Press 1971
Stadleman, E.J., Kinzel, H.: Vital staining of plant cells. In: Methods in cell physiology. pp. 325–372, Ed.: D.M. Prescott, New York: Academic Press 1972
Sweeney, B.M., Hastings, J.W.: Characteristics of diurnal rhythm of luminescence in Gonyaulax polyedra. J. Cell Comp. Physiol. 49, 115–128 (1957)
Sweeney, B.M.: Circadian rhythms in unicellular organisms. In: Proc. Int. Symp. Circadian Rhythmicity, (Wageningen) pp. 137–156 (1971)
Sweeney, B.M.: A freeze-fracture study of Gonyaulax polyedra: A circadian rhythm in membrane particles. J. Phycol. (Suppl.) 11, 19 (1975)
Uvnäs, B., Thon, I.: Evidence for enzymatic histamine release from isolated rat most cells. Exp. Cell Res. 23, 45–57 (1961)
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Adamich, M., Sweeney, B.M. The preparation and characterization of Gonyaulax spheroplasts. Planta 130, 1–6 (1976). https://doi.org/10.1007/BF00390837
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DOI: https://doi.org/10.1007/BF00390837