Abstract
The lipid pattern of Daucus carota L. suspension culture cells and other plant cell strains was analyzed. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and triacylglycerol were the main components. The characteristic plastidal and mitochondrial lipids could also be identified. All strains tested exhibited a phospholipase D activity. Several lipid precursors were found to be well utilized by the cells and to be special markers for certain lipids or parts of the lipid molecules. The half life times of the major lipids ranged at about half a generation time of the cells.
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Bahl, J., Francke, B., Monéger, R.: Lipid composition of envelopes, prolamellar bodies and other plastid membranes in etiolated, green and greening wheat leaves. Planta 129, 193–201 (1976)
Blank, W., Zaar, K., Kleinig, H.: Morphometric measurements of Daucus carota suspension culture cells. Planta 137, 85–87 (1977)
Clark, B.R., Rubin, R.T., Arthur, R.G.: A new micro method for determination of cholesterol in serum. Ann. Biochem. 24, 27–33 (1968)
Comes, P., Kleinig, H.: Phospholipids and phospholipase D in the true slime mold Physarum polycephalum. Biochim. Biophys. Acta 316, 13–18 (1973)
De Silva, N.S., Fowler, M.W.: The lipid composition of Acer pseudoplatanus cells grown in culture. Phytochem. 15, 1735–1740 (1976)
Douce, R.: Sites of biosynthesis of galactolipids in spinach chloroplasts. Science 183, 852–853 (1974)
Duncombe, W.G.: The colorimetric micro-determination of longchain fatty acids. Biochem. J. 88, 7–10 (1963)
Gamborg, O.L., Miller, R.O., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158 (1968)
Gerlach, E., Deuticke, B.: Eine einfache Methode zur Mikrobestimmung von Phosphat in der Papierchromatographie. Biochem. Z. 337, 477–479 (1963)
Hahlbrock, K.: Further studies on the relationship between the rates of nitrate uptake, growth and conductivity changes in the medium of plant cell suspension cultures. Planta 124, 311–318 (1975)
Kleinig, H., Lempert, U.: Phospholipid analysis on a micro scale. J. Chromatog. 53, 595–597 (1970)
Liedvogel, B., Kleinig, H.: Lipid metabolism in chromoplast membranes from the daffodil: glycosylation and acylation. Planta 133, 249–253 (1977)
Mackender, R.O., Leech, R.M.: The galactolipid, phospholipid, and fatty acid composition of the chloroplast envelope membrane of Vicia faba L. Plant Physiol. 53, 496–502 (1974)
Mazliak, P., Douady, D., Demandre, C., Kader, J.C.: Exchange processes between organelles involved in membrane lipid biosynthesis. In: Recent Advances in the Chemistry and Biochemistry of Plant Lipids. Galliard, T., Mercer, E.J., eds., pp. 301–318. London-New York-San Francisco: Academic Press 1975
Poincelot, R.P.: Isolation and lipid composition of spinach chloroplast envelope membranes. Arch. Biochem. 159, 134–142 (1973)
Radwan, S.S., Mangold, H.K.: The lipids of plant tissue cultures. Adv. Lipid Res. 14, 171–211 (1976)
Radwan, S.S., Spener, F., Mangold, H.K.: Lipids in plant tissue cultures. IV. The characteristic pattern of lipid classes in callus cultures and suspension cultures. Chem. Phys. Lipids 14, 72–80 (1975)
Roughan, P.G., Slack, C.R.: Is phopholipase D rearly an enzyme? A comparison of in situ and in vitro activities. Biochim. Biophys. Acta 431, 86–95 (1976)
Slack, C.R., Roughan, P.G., Balasingham, N.: Labelling studies in vivo on the metabolism of the acyl and glycerol moieties of the glycerolipids in the developing maize leaf. Biochem. J. 162, 289–296 (1977)
Song, M., Tattrie, N.H.: Lipid composition of morning glory (Ipomoea sp.) cells grown in suspension cultures. Can. J. Bot. 51, 1893–1897 (1973)
Stearns, E.M., Morton, W.T.: Biosynthesis of fatty acids from acetate in soybean suspension cultures. Lipids 10, 597–601 (1975)
Stumpf, P.K., Weber, N.: Uptake and metabolism of fatty acids by soybean suspension cells. Lipids 12, 120–124 (1977)
Whistler, R.L., Wolfram, M.L.: Methods in Carbohydrate Chemistry. Vol. 1, p. 388. New York-London: Academic Press 1962
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Kleinig, H., Kopp, C. Lipids, lipid turnover, and phospholipase D in plant suspension culture cells (Daucus carota). Planta 139, 61–65 (1978). https://doi.org/10.1007/BF00390811
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DOI: https://doi.org/10.1007/BF00390811