Abstract
The short-term effects of auxin (indole-3-acetic acid) and fusicoccin (FC) on Rb+ uptake and malate accumulation in Avena sativa L. coleoptile sections have been investigated. FC stimulates 86Rb+ uptake within 1 min while auxin-enhanced uptake begins after a 15–20-min lag period. Auxin has little or no effect on 86Rb+ uptake at external pHs of 6.0 or less, but substantial auxin effects can be observed in the range of pH 6.5 to 7.5. Competition studies indicate that the uptake mechanism is specific for Rb+ and K+. After 3 h of auxin treatment the total amount of malate in the coleoptile sections is doubled compared to control sections. FC causes a doubling of malate levels within 60 min of treatment. Auxin-induced malate accumulation exhibits a sensitivity to inhibitors and pH which is similar to that observed for the H+-extrusion and Rb+-uptake responses. Both auxin- and FC-enhanced malate accumulation are stimulated by monovalent cations but this effect is not specific for K+.
Similar content being viewed by others
Abbreviations
- FC:
-
fusicoccin
- IAA:
-
indole-3-acetic acid
References
Allaway, W.G.: Accumulation of malate in guard cells of Vicia faba during stomatal opening. Planta 110, 63–70 (1973)
Burström, H.: Studies on the buffer systems of cells. Ark. Bot. 32A, Nr. 7 (1945)
Cleland, R.E.: Fusicoccin-induced growth and hydrogen ion excretion of Avena coleoptiles: Relation to auxin responses. Planta 128, 201–206 (1976a)
Cleland, R.E.: Rapid stimulation of K+-H+ exchange by a plant growth hormone. Biochem. Biophys. Res. Commun. 69, 333–338 (1976b)
Davies, D.D.: Control of and by pH. Symp. Soc. Exp. Biol. 27, 513–529 (1973)
Haschke, H.P., Lüttge, U.: Stoichiometric correlation of malate accumulation with auxin-dependent K+-H+ exchange and growth in Avena coleoptile segments. Plant Physiol. 56, 696–698 (1975)
Haschke, H.P., Lüttge, U.: Action of auxin on dark CO2 fixation in Avena coleoptile segments as related to elongation growth. Plant Sci. Lett. 8, 53–58 (1977)
Hohorst, H.J.: L-(-)-malate, determination with malic dehydrogenase and DPN. In: Methods of enzymatic analysis, pp. 328–332, Bergmeyer, H.U., ed. Weinheim: Verlag Chemie 1963
Jackson, P.C., Adams, H.R.: Cation-anion balance during potassium and sodium absorption by barley roots. J. Gen. Physiol. 46, 369–386 (1963)
Jackson, P.C., Coleman, N.T.: Fixation of carbon dioxide by plant roots through phosphoenolpyruvate carboxylase. Plant & Soil 11, 1–16 (1959)
Jacoby, B., Laties, G.G.: Bicarbonate fixation and malate compartmentation in relation to salt-induced stoichiometric synthesis of organic acid. Plant Physiol. 47, 525–531 (1971)
Johnson, K.D., Rayle, D.L.: Enhancement of CO2 uptake in Avena coleoptiles by fusicoccin. Plant Physiol. 57, 806–811 (1976)
Lado, P., Rasi-Caldogno, F., Colombo, R., DeMichelis, M.I., Marrè, E.: Effects of monovalent cations on IAA- and FC-stimulated proton-cation exchange in pea stem segments. Plant Sci. Lett. 7, 199–209 (1976)
Marrè, E., Lado, P., Rasi-Caldogno, F., Colombo, R., DeMichelis, M.I.: Evidence for the coupling of proton extrusion to K+ uptake in pea internode segments exposed to fusiococin or auxin. Plant Sci. Lett. 3, 365–379 (1974)
Poole, R.J., Poel, L.W.: Carbon dioxide and pH in relation to salt uptake by beetroot tissue. J. Exp. Bot. 16, 453–461 (1965)
Raschke, K., Humble, G.D.: No uptake of anions required by opening stomata of Vicia faba: guard cells release hydrogen ions. Planta 110, 47–57 (1973)
Rayle, D.L.: Auxin-induced hydrogen secretion in Avena coleoptiles and its implications. Planta 114, 63–73 (1973)
Rubinstein, B., Johnson, K.D., Rayle, D.L.: Calcium-enhanced acidification in oat coleoptiles. In: Regulation of cell membrane activities in plants, pp. 307–316, Marré, E., Ciferri, O., eds. Amsterdam: North-Holland Publ. 1977
Stout, R.G.: Significance of malic acid formation in relation to plant cell elongation in reponse to the growth regulators auxin and fusicoccin. M.Sc. thesis, Cal. State Univ., San Diego, Cal., USA (1976)
Ulrich, A.: Metabolism of non-volatile organic acids in excised barley roots as related to cation-anion balance during salt accumulation. Amer. J. Bot. 28, 526–537 (1941)
Van Steveninck, R.F.M.: Some metabolic implications of the tris effect in beetroot tissue. Aust. J. Biol. Sci. 19, 271–281 (1966)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stout, R.G., Johnson, K.D. & Rayle, D.L. Rapid auxin- and fusicoccin-enhanced Rb+ uptake and malate synthesis in Avena coleoptile sections. Planta 139, 35–41 (1978). https://doi.org/10.1007/BF00390807
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00390807