Summary
Ultrastructural changes occurring in isolated tobacco leaf protoplasts following infection with cowpea chlorotic mottle virus and bromegrass mosaic virus variant 5, have been examined. Only the endoplasmic reticulum/nuclear membrane complex has shown a consistent series of changes in structure and distribution. Both viruses induce proliferation of the endoplasmic reticulum and its modification. The nuclear membrane gives rise to small cytoplasmic vacuoles probably containing nucleic acid by a process of blebbing involving both its inner and outer membrane. This is a prominent feature of CCMV infection. BMV5 infection is particularly characterised by the appearance of local organised arrays of assembled virus particles. The results are discussed in terms of possible nucleocytoplasmic interactions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bancroft, J. B., Lane, L. C.: Genetic analysis of cowpea chlorotic mottle and brome mosaic viruses. J. gen. Virol. 19, 381–389 (1973)
Burgess, J., Motoyoshi, F., Fleming, E. N.: The mechanism of infection of plant protoplasts by viruses. Planta (Berl.) 112, 323–332 (1973a)
Burgess, J., Motoyoshi, F., Fleming, E. N.: Effect of poly-L-ornithine on isolated tobacco mesophyll protoplasts; evidence against stimulated pinocytosis. Planta (Berl.) 111, 199–208 (1973b)
Burgess, J., Watts, J. W., Fleming, E. N., King, J. M.: Plasmalemma fine structure in isolated tobacco mesophyll protoplasts. Planta (Berl.) 110, 291–301 (1973c)
De Zoeten, G. A., Gaard, G., Diez, F. B.: Nuclear vesiculation associated with pea enation mosaic virus-infected plant tissue. Virology 48, 638–647 (1972)
Esau, K., Cronshaw, J.: Relation of Tobacco mosaic virus to the host cells. J. Cell Biol. 33, 665–678 (1967)
Esau, K., Hoefert, L. L.: Cytology of Beet yellows virus infection in Tetragonia. I. Parenchyma cells in infected leaf. Protoplasma (Wien) 72, 255–273 (1971)
Esau, K., Hoefert, L. L.: Development of infection with beet western yellows virus in the sugarbeet. Virology 48, 724–738 (1972)
Frederick, S. E., Newcomb, E. H.: Microbody-like organelles in leaf cells. Science 163, 1353–1355 (1969)
Hibi, T., Yora, K.: Electron microscopy of tobacco mosaic virus infection in tobacco mesophyll protoplasts. Ann. Phytopath. Soc. Japan 38, 350–356 (1972)
Kitajima, W. E., Lauritis, J. A., Swift, H.: Morphology and intracellular localisation of a bacilliform latent virus in sweet clover. J. Ultrastruct. Res. 29, 141–150 (1969)
Langenberg, W. G., Schlegel, D. E.: Localisation of Tobacco mosaic virus in tobacco leaf cells during the early stages of infection. Virology 37, 86–93 (1969)
Larsson, C., Collin, C., Albertsson, P.: The fine structure of chloroplast stroma crystals. J. Ultrastruct. Res. 45, 50–58 (1973)
Motoyoshi, F., Bancroft, J. B., Watts, J. W., Burgess, J.: The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. J. gen. Virol. 20, 177–193 (1973)
Otsuki, Y., Takebe, I.: Infection of tobacco mesophyll protoplasts by cucumber mosaic virus. Virology 52, 433–438 (1973)
Otsuki, Y., Takebe, I., Honda, Y., Matsui, C.: Ultrastructure of infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Virology 49, 188–194 (1972)
Paliwal, Y. C.: Electron microscopy of bromegrass mosaic virus in infected leaves. J. Ultrastruct. Res. 30, 491–502 (1970)
Ragetli, H. W. J., Weintraub, M., Lo, E.: Degeneration of leaf cells resulting from starvation after excision. I. Electron microscopic observations. Canad. J. Bot. 48, 1913–1922 (1970)
Roberts, K., Northcote, D. H.: Ultrastructure of the nuclear envelope; structural aspects of the interphase nucleus of sycamore suspension culture cells. Microscopica acta 71, 102–120 (1971)
Shalla, T. A., Amici, A.: The distribution of viral antigen in cells infected with tobacco mosaic virus as revealed by electron microscopy. Virology 31, 78–91 (1967)
Shalla, T. A., Petersen, L. J.: Infection of isolated plant protoplasts with potato virus X. Phytopathology 63, 1125–1130 (1973)
Takebe, I., Otsuki, Y.: Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proc. nat. Acad. Sci. (Wash.) 64, 843–848 (1969)
Takebe, I., Otsuki, Y., Honda, Y., Nishio, T., Matsui, C.: Fine structure of isolated mesophyll protoplasts of tobacco. Planta (Berl.) 113, 21–27 (1973)
Wolanski, B. S., Chambers, T. C.: The multiplication of lettuce necrotic yellows virus. Virology 44, 582–591 (1971)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Burgess, J., Motoyoshi, F. & Fleming, E.N. Structural changes accompanying infection of tobacco protoplasts with two spherical viruses. Planta 117, 133–144 (1974). https://doi.org/10.1007/BF00390795
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00390795