Summary
The brain-type (BB) isoenzyme of creatine kinase (CK) has recently been shown to be estrogenregulated in human breast cancer cells in vitro. In this study we have correlated the presence of CK-BB, evaluated by immunoperoxidase procedures, with the estrogen receptor (ER) content in 35 primary breast cancers. Of 35 tumors examined, 66% revealed moderate to strong CK-BB immunoreactivity. Staining was exclusively located in the cytoplasm of neoplastic cells. A positive relationship was observed between CK-BB positivity and ER content with ER-rich tumors (>50 fmoles/mg protein) showing a more intense immunoreactivity than ER-poor ones. The results indicate that CK-BB is estrogen-regulated in human breast cancer and suggest that evaluation of this enzyme may be of potential value for the detection of hormone responsive tumors.
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Scambia, G., Santeusanio, G., Panici, P.B. et al. Immunohistochemical localization of creatine kinase BB in primary breast cancer: correlation with estrogen receptor content. J Cancer Res Clin Oncol 114, 101–104 (1988). https://doi.org/10.1007/BF00390493
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DOI: https://doi.org/10.1007/BF00390493