Summary
Human lung tumors from 28 patients were investigated by sucrose density analysis for the presence of cellular retinoic acid-binding proteins. In the tumor-free lung tissue and in most of the 20 squamous cell carcinoma and in 1 small cell carcinoma specimens tested no specific binding of retinoic acid was detectable, whereas these binding proteins were observed in 5 adeno-and 1 large cell carcinoma. The amount of retinoic acid-binding protein was slightly increased in 1 bronchial adenoma considered to be a semimalignant tumor.
These findings suggest that the occurrence of retinoic acid-binding proteins within lung carcinomas may be related to the histological type of lung cancer. Thus, the antineoplastic effects of retinoic acid suspected to be mediated by specific binding proteins are not generally to be expected on lung cancer.
Similar content being viewed by others
References
Bashor MM, Toft DO, Chytil F (1973) In vitro binding of retinol to rat-tissue components. Proc Natl Acad Sci USA 70:3483–3487
Bichler E, Daxenbichler G (1982) Retinoic acid-binding protein in human squamous cell carcinomas of the ORL region Cancer 49:619–622
Bollag W (1971) Therapy of chemically induced skin tumors of mice with Vitamin A palmitate and vitamin A acid. Experientia 27:90–92
Bollag W (1974) Therapeutic effects of an aromatic retinoic acid analog on chemically induced skin papillomas and carcinomas of mice. Eur J Cancer 10:731–737
Chytil F, Ong DE (1976) Mediation of retinoic acid-induced growth and anti-tumor activity. Nature 260:49–51
Clamon GH, Nugent KM, Rossi NP (1981) Cellular retinoic acid-binding protein in human lung carcinomas. JNCI 67:61–63
Fasske E (1984) Das bronchopulmonale Adenokarzinom: Gewebliche Differenzierung und zelluläre Herkunft. Eine licht-und elektronenmikroskopische Analyse. Pathologe 5:3–12
Felix EL, Loyd B, Cohen MH (1975) Inhibition of the growth and development of a transplantable murine melanoma by vitamin A. Science 189:886–888
Jetten AM, Jetten MER (1979) Possible role of retinoic acid binding protein in retinoid stimulation of embryonal carcinoma cell differentiation. Nature 278:180–182
Kistler GS, Peter HJ (1979) Wirkung von zwei Retinoiden (Vitamin-A-Analogen) auf menschliche Bronchuskarzinome in vivo (nu/nu Maus) and in vitro. Schweiz Med Wochenschr 109:847–850
Korenman SG (1968) Radio-ligand binding assay of specific estrogens using a soluble uterine macromolecule. J Clin Endocrinol Metab 28:127–140
Küng WM, Geyer E, Eppenberger U, Huber PR (1980) Quantitative estimation of cellular retinoic acid-binding protein activity in normal, dysplastic, and neoplastic human breast tissue. Cancer Res 40:4265–4269
Lacroix A, Lippman ME (1980) Binding of retinoids to human breast cancer cell lines and their effects on cell growth. J Clin Invest 65:586–591
Lotan R (1980) Effects of vitamin A and its analogs (retinoids) on normal and neoplastic cells. Biochim Biophys Acta 605:33–91
Lotan R, Nicolson GL (1977) Inhibitory effects of retinoic acid or retinyl acetate on the growth of untransformed, transformed, and tumor cells in vitro. J Natl Cancr Inst 59:1717–1722
Lowry OM, Rosebrough NY, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275
Mehta RG, Cerny WL, Moon RC (1980) Distribution of retinoic acid-binding proteins in normal and neoplastic mammary tissues. Cancer Res 40:47–49
Nettesheim P (1980) Inhibition of carcinogenesis by retinoids. Can Med Assoc J 122:757–765
Ong DE, Chytil F (1975a) Retinoic acid-binding protein in rat tissue. J Biol Chem 250:6113–6117
Ong DE, Chytil F (1975b) Specifity of cellular retinol-binding protein for compounds with vitamin A activity. Nature 255:74–75
Ong DE, Chytil F (1976) Presence of cellular retinol, and retinoic acid-binding proteins in experimental tumors. Cancer Lett 2:25–30
Ong DE, Page DL, Chytil F (1975) Retinoic acid-binding protein: occurance in human tumors. Science 190:60–61
Palan PR, Romney SL (1980) Cellular binding proteins for vitamin A in human carcinomas and in normal tissues. Cancer Res 40:4221–4224
Rettura G, Schittek A, Hardy M, Levenson SM, Demetrion A, Seifter E (1975) Antitumor action of vitamin A in mice inoculated with adenocarcinoma cells. J Natl Cancer Inst 54:1489–1491
Ross E, Schatz G (1973) Assay of protein in the presence of high concentrations of sulfhydryl compounds. Anal Biochem 54:304–306
Sani BP, Corbett TH (1977) Retinoic acid-binding protein in normal tissues and experimental tumors. Cancer Res 37:209–213
Sani BP, Banerjee CK, Peckham JC (1980a) I. The presence of binding proteins for retionoic acid and dihydrotestosterone in murine and human colon tumors. Cancer 46:2421–2429
Sani BP, Condon SM, Banerjee CK (1980b) 2. Purification and properties of retinol-and retinoic acid-binding, proteins from a transplantable mouse colon tumor. Biochim Biophys Acta 624:226–236
Schacterle GR, Pollack RL (1973) A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal Biochem 51:654–655
Sporn MB, Dunlop NM, Newton DL, Henderson WR (1976a) Relationships between structure and activity of retinoids. Nature 263:110–113
Sporn MB, Dunlop NM, Newton DL, Smith JM (1976b) Prevention of chemical carcinogenesis by vitamin A and its synthetic analogs (retinoids). Fed Proc 35:1332–1338
Sporn MB, Newton DL, Smith JM, Acton N, Jacobson AE, Brossi A (1979) Retinoids and cancer prevention: The importance of the terminal group of the retinoid molecule in modifying activity and toxicity. In: Griffin AC, Shaw CR (eds) Carcinogens: Identification and mechanism of action. Raven, New York, pp 441–453
Trown PW, Buck MJ, Hansen R (1976) Inhibition of growth and regression of a transplantable rat chondrosarcoma by three retinoids. Cancer Treat Rep 60:1647–1653
Trown PW, Palleroni AV, Bohoslawec O, Richelo BN, Halpern JM, Gizzi N, Geiger R, Lewinski C, Machlin LJ, Jetten A, Jetten MER (1980) Relationship, between binding affinities to cellular retinoic acid-binding protein and in vivo and in vitro properties for 18 retinoids. Cancer Res 40:212–220
Wiggert B, Russell P, Lewis M, Chader G (1977) Differential binding to soluble nuclear receptors and effects on cell viability of retinol and retinoic acid in cultured retinoblastoma cells. Biochem Biophys Res Commun 79:218–225
Wilkoff LJ, Dulmadge EA Chopra DP (1980) Viability of cultured Lewis lung cell populations exposed to β-retinoic acid (40753). Proc Soc Exp Biol Med 163:233–236
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kohl, F.V. Relationship between cellular retinoic acid-binding protein and histology of human lung tumors. J Cancer Res Clin Oncol 108, 357–361 (1984). https://doi.org/10.1007/BF00390473
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00390473