Summary
During x-ray-induced development of malignant lymphomas in mice their urinary excretion of eight modified nucleosides was monitored and the values were compared to the results of the histological examination of the animals at time of their sacrifice.
It was found that the pathologically augmented excretion of modified nucleosides begins as much as several weeks before the malignant lymphomas can be diagnosed clinically. Thus some mice had increased levels of modified nucleosides even 10 weeks before sacrifice, though at the time of sacrifice the histological investigation reveled only some small foci of reticulum cell neoplasm in their spleen.
It is therefore stressed that the usefulness of the determination of urinary modified nucleosides as an early noninvasive screening test for cancer in man and as an in vivo carcinogenicity test should be evaluated.
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Abbreviations
- φ:
-
pseudouridine
- m1A:
-
1-methyladenosine
- m5C:
-
5-methylcytidine
- m1I:
-
1-methylinosine
- m1G:
-
1-methylguanosine
- m2G:
-
N 2-methylguanosine
- m2G:
-
N 2,N2-dimethylguanosine
- ac4C:
-
N 4-acetylcytidine
- mt6A:
-
N-(9-β-d-ribofuranosylpurine-6-yl)N-methylcarbamoyl) threonine
- acp3U:
-
3-(3-amino-3-carboxypropyl) uridine
- SD:
-
standard deviation
References
Clark I, MacKenzie JW, McCoy JR, Lin W (1983) Comparison of urinary modified nucleosides and bases in rats with hepatomas and nephroblastomas. Recent Results Cancer Res 84:388–400
Gauss DH, Sprinzl M (1983) Compilation of tRNA sequences. Nucleic Acid Res 11:r1-r53
Gehrke CW, Kuo KC, Davis GE, Suits RD, Waalkes TP, Borek E (1978) Quantitative high-performance liquid chromatography of nucleosides in biological materials. J Chromatogr 150:445–476
Gehrke CW, Kuo KC, Waalkes TP, Borek E (1979) Patterns of urinary excretion of modified nucleosides. Cancer Res 39:1150–1153
Nass G (ed) (1983) Modified nucleosides and cancer. Recent Results Cancer 84 [whole volume]
Page NP (1977) Concepts of a bioassay program in environmental carcinogenesis. In: Kraybill HF, Mehlmann MA (eds) Advances in modern toxicology. Wiley, New York, pp 87–171 (Environmental cancer, vol 3)
Sachs L (1979) Statistische Methoden. Springer, Berlin Heidelberg New York
Schöch G, Winkler K, Heller-Schöch G, Baisch H (1979) Die Analyse von normalen und methylierten Nucleobasen im Urin als neues Kriterium für Diagnose und Verlauf von Malignomen. Klin Pädiatr 191:197–204
Schöch G, Thomale J, Lorenz H, Suberg H, Karsten U (1980) A new method for the simultanous analysis of unmodified and modified urinary nucleosides and nucleobases by high-performance liquid chromatography. Clin Chim Acta 108:247–257
Thomale J, Nass G (1982) Elevated urinary excretion of RNA catabolites as an early signal of tumor development in mice. Cancer Lett 15:149–152
Thomale J, Luz A, Nass G (1983) Increased levels of RNA catabolites in mice as early indicators for malignant growth. Cancer Detec Prev 6:73–76
Upton AC, Wolff FF, Furth J, Kimball AW (1958) A comparison of the induction of myeloid and lymphoid leukemias in x-radiated RF mice. Cancer Res 18:842–848
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Thomale, J., Luz, A. & Nass, G. Excretion of modified nucleosides during development of malignant lymphomas in mice after whode body irradiation. J Cancer Res Clin Oncol 108, 302–307 (1984). https://doi.org/10.1007/BF00390462
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DOI: https://doi.org/10.1007/BF00390462