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Effekte der Phospholipase D auf den Elektronentransport und die Lipidzusammensetzung isolierter Spinatchloroplasten

The effect of phospholipase D on photochemical activity and lipid composition of isolated spinach chloroplasts

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Summary

Phospholipase D shows short and longtime effects on photochemical activity of isolated spinach chloroplasts. After very short incubations with Phospholipase D (Pl D) the Ferricyanide reduction and Dichlorphenol-idenophenol reduction are 70% to 90% higher than in control chloroplasts. In uncoupled chloroplasts the reduction rates are about 20% higher than in the controls. After one h of incubation time with Phospholipase D the photochemical activity is inhibited and now shows only 40% of the control activity. The effect of Phospholipase D on uncoupled chloroplasts is somewhat lower. After two h of incubation time the control activity decreases to about 50% whereas the PLD-effected activity is reduced to 10% of the initial rates. Cyclic phosphorylation is inhibited by Phospholipase D, presumably because Phospholipase D exerts an uncoupling effect.

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Abbreviations

DCPIP:

Dichlorphenolindophenol

GPG:

Glycerophosphatidylglycerin

GPC:

Glycerophosphatidylcholin

GPE:

Glycerophosphatidyläthanolamin

GPI:

Glycerophosphatidylinosit

MGD:

Monogalactosyldiglycerid

DGD:

Digalactosyldiglycerid

PS:

Phosphatidsäure

Pl:

Phospholipide

PLD:

Phospholipase D

Fecy:

Ferricyanid

Tris:

Tris(hydroxymethyl)-Aminomethan

Tricin:

N-Tris(hydroxymethyl)methyl glycin

tr:

trace

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  1. Botanisches Institut II der Universität Karlsruhe, Kaiserstraße 12, D-7500, Karlsruhe 1, Federal Republic of Germany

    H. Uhrig & M. Tevini

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  1. H. Uhrig
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  2. M. Tevini
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Uhrig, H., Tevini, M. Effekte der Phospholipase D auf den Elektronentransport und die Lipidzusammensetzung isolierter Spinatchloroplasten. Planta 128, 173–178 (1976). https://doi.org/10.1007/BF00390320

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  • DOI: https://doi.org/10.1007/BF00390320

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