Summary
A method is described by which the Na+ and K+ content in 0.5 mm sections of single roots of Hordeum distichon L. and Atriplex hortensis L. can be determined by use of flameless atomic absorption spectroscopy. By this method the longitudinal profiles of K+ and Na+ along low salt roots and roots which had been equilibrated with or grown in K+-free 1 mM Na+-solution were determined. The profiles reveal that high K+/Na+ ratios in the cytoplasm are maintained also in K+-free solutions. In solutions containing 1 mM Na+ a high K+/Na+ selectivity was found to be dependent on sufficient aeration. From the ion profiles the cytoplasmic (110 mM) and vacuolar (20 mM) K+ concentration in low salt barley roots—values which are unobtainable by compartmental analysis—could be estimated.
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Brown, R., Cartwright, P.M.: The absorption of potassium by cells in the apex of the root. J. exp. Bot. 4, 197–221 (1953)
Collander, R.: Selective absorption of cations by higher plants. Plant Physiol. 16, 691–720 (1941)
Cormack, R.G.H.: Development of root hairs in angiosperms II. Bot. Rev. 28, 446–464 (1962)
Epstein, E.: The essential role of clacium in selective cation transport by plant cells. Plant Physiol. 36, 437–444 (1961)
Eshel, A., Waisel, Y.: Variations in uptake of sodium and rubidium along barley roots. Physiol. Plant. 28, 557–560 (1973)
Handley, R., Vidal, R.D., Overstreet, R.: Metabolic and nonmetabolic uptake of sodium in roots of Zea mays. Plant Physiol. 35, 907–912 (1960)
Hooymans, J.J.M.: Role of cell compartments in the redistribution of K and Na ions absorbed by the roots of intact barley plants. Z. Pflanzenphysiol. 73, 234–242 (1974)
Jefferies, R.L.: The ionic relations of seedlings of the halophyte Triglochin maritima L. In: Ion Transport in Plants. Ed.: W.P. Anderson. London-New York: Academic Press 1973
Jennings, D.H.: Halophytes, succulence and sodium in plants—a unified theory. New Phytol. 67, 899–911 (1968)
Jeschke, W.D.: Evidence for a K+-stimulated Na+ efflux at the plasmalemma of barley root cells. Planta (Berl.) 94, 240–245 (1970)
Jeschke, W.D.: Wirkung von K+ auf die Fluxe und den Transport von Na+ in Gerstenwurzeln, K+-stimulierter Na+ Efflux in der Wurzelrinde. Planta (Berl.) 106, 73–90 (1972)
Jeschke, W.D.: K+-stimulated Na+ efflux and selective transport in barley roots. In: Ion Transport in Plants, pp. 285–296, Ed.: W.P. Anderson. London-New York: Academic Press 1973
Kramer, P.J., Wiebe, H.H.: Longitudinal gradients of 32P absorption in roots. Plant Physiol. 27, 661–674 (1952)
LaHaye, P.A., Epstein, E.: Calcium and salt toleration by bean plants. Physiol. Plant. 25, 213–218 (1971)
Ling, G.N.: Elektrische Potentiale lebender Zellen. In: Die Zelle, Struktur und Funktion. Ed.: H. Metzner. Stuttgart: Wiss. Verlagsges. 1971
MacRobbie, E.A.C.: Phloem translocation. Biol. Rev. 46, 429–481 (1971)
Neirinckx, L.J.A., Bange, G.G.J.: Irreversible equilibration of barley roots with Na+ ions at different external Na+ concentrations. Acta Bot. Neerl. 20, 481–488 (1971)
Pierce, W.S., Higinbotham, N.: Compartments and fluxes of K+, Na+ and Cl− in Avena coleoptile cells. Plant Physiol. 46, 666–673 (1970)
Pitman, M.G.: Sodium and potassium uptake by seedlings of Hordeum vulgare. Aust. J. biol. Sci. 18, 10–24 (1965)
Pitman, M.G., Saddler, H.D.W.: Active sodium and potassium transport in cells of barley roots. Proc. nat. Acad. Sci. (Wash.) 57, 44–49 (1967)
Rosene, H.F.: A comparative study of the rates of water influx into the hairless epidermal surface and the root hairs of onion roots. Physiol. Plant. 7, 676–686 (1954)
Scott, B.I.H., Gulline, H., Pallaghy, C.K.: The electrochemical state of cells of broad bean roots. I. Investigations of elongating roots of young seedlings. Aust. J. biol. Sci. 21, 185–200 (1968)
Shepherd, U.H., Bowling, D.J.F.: Active accumulation of sodium by roots of five aquatic species. New Phytol. 72, 1075–1080 (1973)
Spanswick, R.M., Williams, E.J.: Electrical potentials and Na, K, and Cl concentrations in the vacuole and cytoplasms of Nitella translucens. J. exp. Bot. 15, 193–200 (1964)
Steward, F.C., Prevot, P., Harrison, J.A.: Absorption and accumulation of rubidium bromide by barley plants. Localization in the root of cation accumulation and of transfer to the shoot. Plant Physiol. 17, 411–421 (1942)
Sun, C.N.: Growth and development of primary tissues in aerated and non-aerated roots of soybean. Bull. Torrey Bot. Club 82, 491–502 (1955) (quoted from Cormack)
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Jeschke, W.D., Stelter, W. Mesurement of longitudinal ion profiles in single roots of Hordeum and Atriplex by use of flameless atomic absorption spectroscopy. Planta 128, 107–112 (1976). https://doi.org/10.1007/BF00390311
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DOI: https://doi.org/10.1007/BF00390311