Skip to main content
SpringerLink
Log in

Untersuchungen am Phloemexsudat von Cucurbita pepo L.

I. Enzymaktivitäten von Glykolyse, Gärung und Citrat-Cyclus

Studies on the phloem exudate of Cucurbita pepo L.

I. Enzyme activities of aerobic and anaerobic glycolysis and of the citric acid cycle

  • Published:
Planta Aims and scope Submit manuscript

Summary

In the phloem exudate of Cucurbita pepo enzyme activities of aerobic and anaerobic glycolysis as well as of citric acid cycle were tested. All enzymes of the aerobic glycolysis and the alcoholic fermentation were present. In the phloem these metabolic pathways should be functioning as well. Since the lactate dehydrogenase could not be detected all the time, lactate fermentation is probably not an obligatory constituent of phloem metabolism. Of the citric acid cycle the enzymes malate dehydrogenase, isocitrate dehydrogenase, aconitase, fumarase and succinate dehydrogenase were analyzed. The activities decrease in the given order of enzymes. Succinate dehydrogenase could not be detected at all. The possibility of finding the citric acid cycle respectively mitochondria in the exudate is discussed. The results do not suggest the presence of such a metabolic pathway in the exudate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Becker, D., Kluge, M., Ziegler, H.: Der Einbau von 32PO in organische Verbindungen durch Siebröhrensaft. Planta (Berl.) 99, 154–162 (1971)

    Google Scholar 

  • Brosemer, R. W., Vogell, W., Bücher, T.: Morphologische und enzymatische Muster bei der Entwicklung indirekter Flugmuskeln bei Locusta migratoria. Biochem. Z. 338, 854–873 (1963)

    Google Scholar 

  • Bücher, T., Luh, W., Pette, D.: Einfache und zusammengesetzte optische Tests mit Pyridinnucleotiden. In: Hoppe-Seyler/Thierfelder, Handbuch der physiologischen und pathologisch-chemischen Analyse, Bd. VI/A, S. 292–339, 10. Aufl., Lang, K., Lehnartz, E. (Hrsg.). Berlin-Heidelberg-New York: Springer 1964

    Google Scholar 

  • Curtis, O. F.: Studies on solute translocation in plants. Experiments indicating that translocation is dependent on the activity of living cells. Amer. J. Bot. 16, 154–168 (1929)

    Google Scholar 

  • Eschrich, W., Evert, R. F., Heyser, W.: Proteins of the sieve-tubes exudate of Cucurbita maxima. Planta (Berl.) 100, 208–221 (1971)

    Google Scholar 

  • Gardner, D. C., Peel, A. J.: Metabolism and transport of 14C-labelled glutamiè and aspartic acids in the phloem of willow. Phytochemistry 10, 2385–2387 (1971)

    Google Scholar 

  • Kennecke, M., Ziegler, H., Fekete, M. A. R. de: Enzymaktivitäten im Siebröhrensaft von Robinia pseudoaccacia L. und anderer Baumarten. Planta (Berl.) 98, 330–356 (1971)

    Google Scholar 

  • Kollmann, R., Dörr, I., Kleinig, H.: Protein filaments — Structural components of the phloem exudate. I. Observations with Cucurbita and Nicotiana. Planta (Berl.) 95, 86–94 (1970)

    Google Scholar 

  • Kursanow, A. L., Sokolova, S. V., Turkina, M. V.: Hexokinase in conducting tissues of sugar-beet and its possible connection with transpor of sugars trough cell membranes. J. exp. Bot. 21, 30–39 (1970)

    Google Scholar 

  • Lehmann, J.: Zur Lokalisation von Dehydrogenasen im Phloem von Cucurbita pepo (L.). Planta (Berl.) 111, 187–198 (1973a)

    Google Scholar 

  • Lehmann, J.: Untersuchungen am Phloemexsudat von Cucurbita pepo (L.). II. Enzymaktivitäten der Gluconeogenese und des Auf- und Abbaus von Di- und Polysacchariden Planta (Berl.) 114, 51–61 (1973b)

    Google Scholar 

  • Pette, D.: Plan und Muster im zellulären Stoffwechsel. Naturwissenschaften 52, 597–616 (1965)

    Google Scholar 

  • Pette, D., Brandau, H.: Enzymhistiogramme und Enzymaktivitätsmuster in der Rattenleber. Enzym. biol. clin. 6, 79–122 (1966)

    Google Scholar 

  • Pette, D., Luh, W., Bücher, T.: A constant-proportion group in the enzyme activity pattern of Emden-Meyerhof chain. Biochem. biophys. Res. Comm. 7, 419–424 (1962)

    Google Scholar 

  • Schumacher, W.: Untersuchungen über die Lokalisation der Stoffwanderung in den Leitbündeln der höheren Pflanzen. Jb. wiss. Bot. 73, 770–823 (1930)

    Google Scholar 

  • Ullrich, W.: Zur Sauerstoffabhängigkeit des Transportes in den Siebröhren. Planta (Berl.) 57, 402–429 (1961)

    Google Scholar 

  • Willenbrink, J.: Zur lokalen Hemmung des Assimilattransportes durch Blausäure. Z. Pflanzenphysiol. 55, 119–130 (1966)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lehrstuhl für Zellbiologie, Botanisches Institut der Universität Kiel, Düsternbrooker Weg 17, D-2300, Kiel, Federal Republic of Germany

    Johannes Lehmann

Authors
  1. Johannes Lehmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lehmann, J. Untersuchungen am Phloemexsudat von Cucurbita pepo L.. Planta 114, 41–50 (1973). https://doi.org/10.1007/BF00390283

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00390283

Search

Navigation