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Formation of chloroplast pigments and sterols in rye leaves deficient in plastid ribosomes

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1. In rye (Secale cereale L.) leaves the formation of plastidic ribosomes is sensitive to elevated growth temperatures. Parallel to the loss of 70S ribosomes, in leaves growing at 32° chlorophyll accumulation was also prevented. Except for the tips of the first leaves which still contained some 70S ribosomes, the leaves were chlorotic. The amount of chlorophyll formed at 32° depended on the light intensity and decreased with higher intensities. After return to normal temperature (22°) chlorotic parts of the first leaves greened to a varying extent while those parts of most 2. or 3. leaves which had been formed in light at 32° remained permanently bleached until they died. Those parts of 2. and 3. leaves which were newly formed at 22° became normally green again. — 2. Formation and distribution of total and individual carotenoids were compared after development at 22° and 32°. In dark-grown leaves the higher growth temperature had no marked influence on the quantity or composition of carotenoids. At 22° the content of total carotenoids was 5fold and that of β-carotene 25fold increased by light. At 32° these light-induced increases were much lower. Only 41% of the total carotenoids and 18% of the β-carotene formed at 22° in light were found at 32°. Of the carotenoids present at 32°, 76% were located in the light green tips of the leaves. In plastids isolated from completely chlorotic leaf parts, carotenoids were still present and were even the predominant pigments. — 3. The contents of total sterols, the fractions of free sterols, sterol glycosides and esters, and the composition of individual sterols were compared in rye leaves grown at 22° and at 32°, in light or darkness. Light had little effect on the total sterol contents per leaf. However, more than 2fold higher sterol contents were observed in leaves grown at 32°, as compared to those from 22°. The amounts of most sterol fractions and individual sterols were similarly increased at the higher temperature but the sterol glycosides being relatively more increased than the total sterols.

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  1. Lehrstuhl für Pflanzenphysiologie, Arbeitsgruppe Pflanzliche Zellphysiologie der Ruhr-Universität Bochum, Postfach 2148, D-4630, Bochum, Federal Republic of Germany

    Elfriede Rademacher & J. Feierabend

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  1. Elfriede Rademacher
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  2. J. Feierabend
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Rademacher, E., Feierabend, J. Formation of chloroplast pigments and sterols in rye leaves deficient in plastid ribosomes. Planta 129, 147–153 (1976). https://doi.org/10.1007/BF00390021

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