Summary
According to our hypothesis, organ-specific lectins (e.g., the d-galactose-specific hepatic binding protein) play an important role in the organ location of metastatic malignant cells. The rapid clearance and uptake by the liver of tritiated α-acid-(asialo)glycoprotein from the circulation of Balb/c mice was markedly delayed after preinjection of d-galactose or arabinogalactan. The preinjection (1h) and regular application (for 3 days after tumor cell inoculation in Balb/c mice) of the receptor blocking agents d-galactose and arabinogalactan prevented the settling of sarcoma L-1 tumor in the liver completely, but did not influence the settling in the lung. Other galactans, dextrans, and phosphate-buffered saline showed no effect. Therefore, when lectins were blocked with competitive-specific glycoconjugates, colonization was prevented.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ashwell G, Morell AG (1974) The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol 41:99–128
Baenziger JU (1985) The role of glycosylation by protein recognition. Am J Pathol 121:382–391
Beuth J, Uhlenbruck G (1985) Leber-Lektine als Mediatoren der Metastasierung maligner Tumoren. Funkt Biol Med 5:269–275
Beuth J, Oette K, Uhlenbruck G (1986) Liver lectins as receptors for tumor cells in metastasis. In: Van Driessche E, Bøg-Hansen TC (eds) Lectins, vol 5. Walter de Gruyter, Berlin, pp 229–237
Fidler IJ (1985) Macrophages and Metastasis—A biological approach to cancer therapy. Cancer Res 45:4714–4726
Fidler IJ, Hart JR (1982) Biological diversity in mettastatic neoplasma: origins and implications. Science 217:998–1003
Fidler IJ, Poste G (1982) Macrophage-mediated destruction of malignant tumort cells and new strategies for the therapy of metastatic disease. Springer Semin Immunopathol 5:161–174
Gabius HJ, Engelhardt R, Cramer F (1986) Endogenous tumor lectins: a new class of tumor markers and target for therapy. Med Hypotheses 18:47–53
Hakomori SI (1985) Aberrant glycosylation in cancer cell membranes as focused on glycolipids: overview and perspectives. Cancer Res 45:2405–2414
Hanfland P, Uhlenbruck G (1978) Aktuelle immunchemische Betrachtungen zum Aufbau der Tumorzellmembran. J Clin Chem Clin Biochem 16:85–101
Hill RP, Bush RS (1969) A lung colony assay to determine radiosensitivity of the cells of a solid tumor. Int J Radiat Biol 15:435–444
Larionova NI, Mityushina GV, Kazanskaya NF, Blidchenko YA, Berezin IV (1985) Carbohydrate-containing derivatives of the Trypsin-Kallikrein inhibitor Aprotinin from bovine organs. Biol Chem Hoppe-Seyler 366:743–748
Pricer WE, Ashwell G (1971) The binding of desialylated glycoproteins by plasma membranes of rat liver. J Biol Chem 246:4825–4831
Roszkowski K, Ko HL, Roszkowski W, Jeljaszewicz J, Pulverer G (1985) Effect of some antimicrobial antibiotics on sarcoma L-1 tumor growth in mice. Zbl Bakt Suppl 13:199–213
Schirrmacher V (1984) Tumor cell properties of importance for metastasis. Verh. Dtsch Ges Pathol 68:12–17
Schirrmacher V, Altevogt P, Fogel M, Dennis J, Waller CA, Barz D, Schwartz R, Cheingsong-Popov R, Springer G, Robinson PJ, Nebe T, Brossmer W, Vlodavsky J, Paweletz N, Zimmermann HP, Uhlenbruck G (1982) Importance of cell surface carbohydrates in cancer cell adhesion, invasion and metastasis. Does sialic acid direct metastatic behaviour? Invasion Metastasis 2:313–360
Sharon N (1984) Lectin-like bacterial adherence to animal cells. In: Boedeker EC (ed) Attachment of organisms to the gut mucosa, vol 1. CRC Press, Boca Raton, FL, pp 129–147
Springer GF, Desai PR, Banatwala J (1975) Blood group MN antigens and precursors in normal and malignant human breast glandular tissue. J Natl Cancer Inst 54:335–339
Springer GF, Cheingsong-Popov R, Schirrmcher V, Desai PR, Tegtmeyer H (1982) Proposed molecular basis of murine cell hepatocyte interaction. J Biol Chem 9:5702–5706
Springer GF, Desai PR, Tegtmeyer H, Schirrmacher V, Cheingsong-Popov R (1983) Murine lymphoma cells possess blood group Tn-, T-, N-, M-, and S-active substances. Naturwiss 70:98–108
Uhlenbruck G (1981) The Thomsen-Friedenreich (TF) receptor: an old history with new mystery. Immunol Commun 10:251–264
Uhlenbruck G (1982) Die Biologie der Lektine: eine biologische Lektion. Funkt Biol Med 2:40–48
Uhlenbruck G, Wintzer G, Kania J, Koch O (1979) Immunologische und klinisch-diagnostische Studien an tumor-assoziierten Antigenen. Forschungsberichte des Landes NRW, Nr2903, Westdeutscher Verlag, Opladen
Uhlenbruck G, Beuth J, Weidtman V (1983a) Liver-lectins: mediators for metastases? Experientia 39:1314–1315
Uhlenbruck G, Hartmann Ch, Koch OM (1983b) Adherence properties of Pseudomonas pili to epithelial cells of the human cornea: additional, “lectinological” aspects. Curr Eye Res 4:641–643
Uhlenbruck G, Lütticken R, Bäz K, Böhmer G (1985) Serologische Kreuzreaktionen von Gruppe-B-Streptokokken. Immun Infekt 13:275–282
Uhlenbruck G, Beuth J, Oette K, Schotten T, Ko HL, Roszkowski K, Roszkowski W, Lütticken R, Pulverer G (1986a) Lektine und die Organotropie der Metastasierung. Dtsch Med Wschr 111:991–995
Uhlenbruck G, Beuth J, Oette K, Roszkowski W, Ko HL, Pulverer G (1986b) Prevention of experimental liver metastases by arabinogalactan. Naturwiss 73:626–627
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beuth, J., Ko, H.L., Oette, K. et al. Inhibition of liver metastasis in mice by blocking hepatocyte lectins with arabinogalactan infusions and d-galactose. J Cancer Res Clin Oncol 113, 51–55 (1987). https://doi.org/10.1007/BF00389966
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00389966