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Changes in intestinal mucosa above lymph follicles during carcinogenesis in rats

A light and electron microscopic study

  • Original Papers
  • Experimental Oncology
  • Published:
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Summary p Changes in the intestinal mucosa during carcinogenesis were investigated in 36 rats after weekly s.c. injection of 20 mg dimethylhydrazine/kg body-weight. More changes were seen in the large than in the small intestine. In the first week, 60% of colonic lymphoid plaques displayed various crypt abscesses and glandular regenerations. These mucosal changes correspond to the glands covering the lymph follicles, in direct contact with lymphoid cells.

Beginning in week 8, dysplastic glands developed in these mucosal areas above the lymph follicles. The number of lymphoid plaques with dysplastic glands in the large intestine increased week by week, attaining 75% in week 20. At the end of week 12 the first adenocarcinoma was detected in the cecum by light microscopy, and classified as a poorly differentiated adenocarcinoma with signet ring cells infiltrating the lymph follicles which contained endocrine cells. The majority of adenocarcinomas (10 cases) occurred in week 20. of these, 7 were localized above the lymphatic plaques in the intestine.

Endocrine cells were found in varying numbers in 6 of 10 adenocarcinomas. Three endocrine cell carcinomas, corresponding to human adenocarcinoids or goblet cell carcinoids, developed within the intestinal mucosa; all were identified as poorly differentiated intestinal adenocarcinomas, two of them situated above lymph follicles. These suprafollicular tumors developing from the glandular base, were composed of mucoid cells, endocrine cells, and undifferentiated cells. Microcarcinomas are considered as initial stages of endocrine cell carcinoma.

The trend of tumor development above colonic lymph follicles, and the histogenesis of endocrine cell carcinomas and de novo carcinomas is discussed.

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Authors and Affiliations

  1. Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität Münster, Domagkstrasse 17, D-4400, Münster, Federal Republic of Germany

    F. Shimamoto & E. Vollmer

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  1. F. Shimamoto
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  2. E. Vollmer
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Dedicated to Professor E. Grundmann on the occasion of his 65th birthday, September 28, 1986

Supported by A. v. Humboldt-Stiftung and Ministerium für Wissenschaft und Forschung NRW, grant IV B5-FA 9843

Fellow of the Alexander-von-Humboldt-Stiftung

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Shimamoto, F., Vollmer, E. Changes in intestinal mucosa above lymph follicles during carcinogenesis in rats. J Cancer Res Clin Oncol 113, 41–50 (1987). https://doi.org/10.1007/BF00389965

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  • DOI: https://doi.org/10.1007/BF00389965

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