Summary
Promastigotes of Leishmania braziliensis guyanensis cultivated in the NNN medium agglutinate with concanavalin A (con A). The protozoon was agglutinated at different concentrations of con A. Maximal agglutination was obtained with 150 μg/ml. Three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body.
Cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. A con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect con A receptors on the cell membrane of the parasite.
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References
Aleman, C.: Fine structure of cultured Leishmania braziliensis. Exp. Parasit. 24, 259–264. (1969)
Allsop, B. A., Njogu, A. R.: Monosaccharide surface glycoprotein of Trypanosoma brucei Parasitology 69, 271–281 (1974)
Alves, M. J. M., Colli, W.: Agglutination of Trypanosoma cruzi by concanavalin A. J. Protozool. 21, 575–578 (1974)
Bernhard, W., Avrameas, S.: Ultrastructural visualization of cellular carbohydrate components by means of concanavalin A. Exp. Cell Res. 64, 232–236 (1971)
Chatterjee, S. W., Sen Gupta, P. C.: Ultrastructure of the promastigotes of Leishmania donovani. Indian J. med. Res. 58, 70–76 (1970)
De Souza, W.: Cytochemical detection of polysaccharides on the cell coat of trypanosomatids. Trans. roy. Soc. trop. Med. Hyg. 69, 361 (1975)
De Souza, W.: Cytochemical detection of carbohydrates in the Golgi complex of Leptomonas pessoai. Z. Parasitenk. 1976 (in press)
De Souza, W., Bunn, M. M., Angluster, J.: Surface components of Leptomonas pessoai. An electron microscopic and cytochemical demonstration of concanavalin A receptors on the cell membrane J. Protozool. 1976 (in press)
De Souza, W., Grynberg, N,. Nery-Guimarães, F.: Aspectors ultraestruturais da forma epimastigota do Trypanosoma cruzi em meio LIT. Rev. Soc. bras. Med. trop. (1975) (in press)
De Souza, W., Meyer, H.: An electron microscopic and cytochemical study of the cell coat of Trypanosoma cruzi in tissue cultures Z. Parasitenk. 46, 170–187 (1975)
Dwyer, D. M.: Lectin binding saccharides on a parasitic protozoan. Science 184, 471–473 (1974)
Dwyer, D. M., D'Alesandro, P. A.: Cell surface carbohydrates in Trypanosoma duttoni. J. Protozool. 21, 430a (1974) (abstr.)
Dwyer, D. M., Langreth, S. G., Dwyer, N. K.: Evidence for a polysaccharide surface coat in the developmental stages of Leishmania donovani. A fine structure cytochemical study. Z. Parasitenk. 43, 227–249 (1974)
Gardener, P. J.: Pellicle-associated structures in the amastigote stage of Trypanosoma cruzi and Leishmania species. Ann. trop. Med. Parasit. 68, 167–176 (1974)
Graham, R. C., Karnovsky, M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966)
Inoki, S., Nakanishi, K., Nakabayashi, T.: Study of Leishmania donovani with special reference to the kinetoplast, mitochondria and Golgi zone by electron microscope employing the thin section technique. Biken's J. 1, 194–197 (1958)
Jadin, J. N., Creemers, J.: Etude de l'ultrastructure et de la biologie de Leishmania mexicana Biagi, 1963. II. Les formes en rosaces. Bull. Soc. Path. exot. 60, 58–60 (1967)
Killick-Kendrick, R., Molyneux, D. H., Ashford, R. W.: Leishmania in phlebotomid sandflies. I. Modifications of the flagellum associated with attachment to the mid-gut and oesophageal valve of the sandfly. Proc. roy. Soc. B 187, 409–419 (1974)
Lainson, R., Shaw, J. J.: Leishmaniasis of the new world: Taxonomic problems. Brit. med. Bull. 28, 44–48 (1972)
Lewis, D. H.: Ultrastructural study of promastigotes of Leishmania from reptiles. J. Protozool. 22, 344–352 (1975)
Luft, J. H.: Improvement in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)
Martinez-Palomo, A.: The surface coat of animal cells. Int. Rev. Cytol. 29, 29–75 (1970)
Martinez-Palomo, A., De Souza, W., Gonzales-Robles, A.: Topographical heterogeneity of surface coat and membrane particles of Trypanosoma cruzi. J. Cell Biol. 67, 264a (1975) (abstr.)
Martinez-Palomo, A., De Souza, W., Gonzales-Robles, A.: Topographical heterogeneity of surface coat and membrane particles in Trypanosoma cruzi. Cytochemistry and freezefracture of culture forms. J. Cell Biol. 1976 (in press)
Martinez-Palomo, A., Gonzales-Robles, A., De La Torres, M.: Selective agglutination of pathogenic strains of Entamoeba histolytica induced con A. Nature (Lond.) 245, 186–187 (1973)
Pham, T. D., Azar, H. A., Moscovic, E. A., Kurban, A. K.: The ultrastructure of Leishmania tropica in the oriental sore. Ann trop. Med. Parasit. 64, 1–4 (1970)
Pinto da Silva, P., Martinez-Palomo, A., Gonzales-Robles, A.: Membrane structure and surface coat of Entamoeba histolytica. Topochemistry and dynamics of the cell surface: Cap formation and microexudate. J. Cell Biol. 64, 538–550 (1975)
Rambourgh, A.: Morphological and histochemical aspects of glycoproteins at the surface of animal cells. Int. Rev. Cytol. 29, 57–113 (1971)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–213 (1963)
Rudzinska, M. A., D'Alesandro, P. A., Trager, W.: The fine structure of Leishmania donovani and the role of the kinetoplast in the Leishmania-leptomonad transformation. J. Protozool. 11, 166–191 (1964)
Sanyal, A. B., Sen Gupta, P. C.: Fine structure of Leishmania in dermal leishmanoid. Trans. roy. Soc. trop. Med. Hyg. 61, 211–216 (1967)
Steiger, R.: Some aspects of the surface coat formation in Trypanosoma brucei. Acta trop. 28, 341–346 (1971)
Steiger, R.: Ultracytochemistry of the surface coat/pellicle complex in Trypanosoma brucei. Acta trop. 32, 152–158 (1975)
Tay, J.: Descripcion de Leishmania mexicana mediante el microscopio eletrônico. Pren. méd. mex. 31, 294–305 (1966)
Thiéry, J. P.: Mise en évidence des polysaccharides sur coupes fines en microscopie eletronique. J. Microscopie 6, 987–1018 (1967)
Trager, W., Rudzinska, M. A.: The riboflavin requirement and the effects of acriflavin on the fine structure of the kinetoplast of Leishmania tarentolae. J. Protozool. 11, 133–145 (1964)
Vickerman, K.: On the surface coat and flagellar adhesion in trypanosoma. J. Cell Sci. 5, 163–193 (1969)
Wright, K. A., Hales, H.: Cytochemistry of the pellicle of blood stream forms of Trypanosoma (Trypanozoon) brucei. J. Parasit. 56, 671–683 (1970)
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This work has been supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Conselho de Ensino e Pesquisa da U.F.R.J. and Banco National de Desenvolvimento Econômico, FUNTEC-241
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De Souza, W., Brasil, R.P. An electron microscopic and cytochemical detection of concanavalin a receptors on the cell membrane of Leishmania braziliensis guyanensis . Z. F. Parasitenkunde 50, 1–9 (1976). https://doi.org/10.1007/BF00389927
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DOI: https://doi.org/10.1007/BF00389927