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Salinity effects on glucose uptake and catabolism in the obligately psychrophilic marine bacterium Vibrio marinus

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Abstract

Studies on the effects of various salinities on the uptake and catabolism of glucose in Vibrio marinus MP-1 revealed several significant shifts in total uptake and respiration as the cells were subjected to increasingly greater concentrations of NaCl. As the salinity increased from 0.30 to 1.0 M NaCl, there was a decrease in the C6/C1 (CO2) ratio. The resulting patterns suggests that the relative participation of the hexose monophosphate pathway in glucose catabolism was altered. This pathway is apparently shut down in the region of the minimum-growth salinity, and may be related to growth limitation at rower salinities. The shift in C6/C1 ratio was not affected by changing the incubation temperature, nor was it dependent specifically on the presence of Na+ or Cl-. As the salinity increased from 0.15 to 0.30 M NaCl, there was a shift in the total uptake patterns which suggests the formation and loss of metabolic by-products derived from the first, second, sixth, and presumably fifth carbons of glucose.

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Literature cited

  • Bulla, L. A., G. St. Julian, R. A. Rhodes and C. W. Hesseltine: Physiology of sporeforming bacteria associated with insects. I. Glucose catabolism in vegetative cells. Can. J. Microbiol. 16, 243–248 (1970).

    PubMed  Google Scholar 

  • Christian, W.: Aldolase from yeast. In: Methods in enzymology 1965, pp 315–320. Ed. by S. P. Colowick and N. O. Kaplan. New York. Academic Press 1955.

    Google Scholar 

  • Colwell, R. E. and R. Y. Morita: Reisolation and emendation of description of Vibrio marinus (Russell). Ford. J. Bac 88, 831–837 (1964).

    Google Scholar 

  • Cooper, M. F. and R. Y. Morita: Interaction of salinity and temperature on net protein synthesis and viability of Vibrio marinus. Limnol. Oceanogr. 17, 556–565 (1972).

    Google Scholar 

  • Hamilton, R. D. and J. E. Preslan: Observations on heterotrophic activity in the Eastern Tropical Pacific. Limnol. Oceanogr. 15, 395–401 (1970).

    Google Scholar 

  • MacLeod, R. A., C. A. Claridge, A. Hori and J. F. Murray: Observations of the function of sodium in the metabolism of a marine bacterium. J. biol. Chem. 232, 829–834 (1958).

    PubMed  Google Scholar 

  • Morita, R. Y. and R. D. Haight: Temperature effects on the growth of an obligate psychrophilic marine bacterium. Limnol. Oceanogr. 9, 103–106 (1964).

    Google Scholar 

  • O'Brien, R. W. and J. R. Stern: Requirement for sodium in the anaerobic growth of Aerobacter aerogenes on citrate. J. Bact. 98, 388–393 (1969a).

    PubMed  Google Scholar 

  • ——: Role of sodium in determining alternate pathways of aerobic citrate catabolism in Aerobacter aerogenes. J. Bact. 99, 389–394 (1969b).

    PubMed  Google Scholar 

  • Payne, W. J.: Effects of sodium and potassium ions on growth and substrate penetration of a marine pseudomonad. J. Bact. 80, 696–700 (1960).

    PubMed  Google Scholar 

  • Stanley, S. O. and R. Y. Morita: Salinity effect on the maximum growth temperature of some bacteria isolated from marine environments. J. Bact. 95, 169–173 (1968).

    PubMed  Google Scholar 

  • Stevenson, J.: The specific requirement for sodium chloride for the active uptake of L-glutamate by Halobacterium salinarium. Biochem. J. 99, 257–260 (1966).

    PubMed  Google Scholar 

  • Wang, C. H. and J. K. Krackov: The catabolic fate of glucose in Bacillus subtilis. J. biol. Chem. 237, 3614–3622 (1967).

    Google Scholar 

  • —, I. Stern, E. M. Gilmour, S. Klungsoyr, D. J. Reed, J. J. Bialy, B. E. Christensen and V. H. Cheldelin: Comparative study of glucose catabolism by the radiorespirometric method. J. Bact. 76, 207–216 (1958).

    PubMed  Google Scholar 

  • Wong, P. T. S., T. Thompson and R. A. MacLeod: Nutrition and metabolism of marine bacteria. XVII. Ion dependent retention of α-aminoisobutyric acid and its relation to Na+ dependent transport in a marine bacterium. J. biol. Chem. 244, 1016–1025 (1969).

    PubMed  Google Scholar 

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Author notes

  1. R. P. Griffiths

    Present address: Department of Microbiology, Oregon State University, Corvallis, Oregon, USA

Authors and Affiliations

  1. Department of Microbiology, Oregon State University, Corvallis, Oregon, USA

    R. Y. Morita

  2. School of Oceanography, Oregon State University, Corvallis, Oregon, USA

    R. P. Griffiths & R. Y. Morita

Authors
  1. R. P. Griffiths
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  2. R. Y. Morita
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Additional information

Communicated by J. Bunt, Miami

This paper was taken in part from a dissertation by the senior author, submitted in partial fulfillment of the requirement for the Ph.D. degree, Oregon State University, Corvallis. Published as technical paper No. 3647, Oregon Agricultural Experiment Station.

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Griffiths, R.P., Morita, R.Y. Salinity effects on glucose uptake and catabolism in the obligately psychrophilic marine bacterium Vibrio marinus . Mar. Biol. 23, 177–182 (1973). https://doi.org/10.1007/BF00389482

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  • DOI: https://doi.org/10.1007/BF00389482

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