Summary
DNA enriched for supercoiled plasmids prepared from the 3 μm plasmid-enriched, [ψ +], [2 μm°] strain 6-1G-P188 and from the [2 μm+] [ψ+] strain LL20 can be used to transform a ψ − recipient strain to ψ +. Fractionation of the former preparation by electrophoresis showed that the 3 Mm plasmid band contained the transforming activity.
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References
Clark-Walker GD, Azad AA (1980) Nucleic Acids Res 8:1009–1022
Conde J, Fink GR (1976) Proc Natl Acad Sci USA 73:3651–3655
Cox BS (1965) Heredity 20:505–521
Cox BS, Bevan EA (1962) New Phytol 61:342–356
Cox BS, Tuite MF, Mundy CR (1980) Genetics 85:598–609
Cryer DR, Eccleshall R, Marmur J (1975) In: Prescott, DM (ed) Methods in cell biology, vol 12. Academic Press, London, pp 39–44
Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
Hsaio C-L, Carbon J (1979) Proc Natl Acad Sci USA 76:3829–3833
Larionov VL, Grishin AV, Smirnov MN (1980) Gene 12:41–49
Liebman S, Sherman F (1979) J Bacteriol 139:1068–1071
Lund PM (1982) D Phil thesis Oxford
Lund PM, Cox BS (1981) Genet Res Camb 37:173–182
Skryabin KG, Elderov MA, Larionov VL, Bayev AA, Klootwijk J, de Regt VCHR, Veldam GM, Planta RJ, Georgiev OI, Hadjiolov AA (1984) Nucleic Acids Res 12:2955–2968
Southern EM (1975) J Mol Biol 98:503–517
Szostak JW, Wu R (1979) Plasmid 2:536–554
Tuite MF, Mundy CR, Cox BS (1981) Genetics 98:691–711
Tuite MF, Lund PM, Futcher AB, Dobson MJ, Cox BS, McLaughlin CS (1982) Plasmid 8:103–111
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Dai, H., Tsay, S.H., Lund, P.M. et al. Transformation of ψ − Saccharomyces cerevisiae to ψ + with DNA co-purified with 3 μm circles. Curr Genet 11, 79–82 (1986). https://doi.org/10.1007/BF00389429
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DOI: https://doi.org/10.1007/BF00389429