Summary
The plasmalemmata of root tip cells of Phaseolus vulgaris L. appear to be well preserved in freeze-fractured material in which no glycerol pre-treatment has been used. The plasmalemmata retain the impressions made by the microfibrils and pit-fields of the cell walls against which these membranes are pressed in turgid cells. Material freeze-fractured in this way is therefore more suitable than glycerol-treated material (in which these impressions are not retained) for the study of membrane features which may be related to microfibril synthesis.
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Willison, J.H.M. Plant cell-wall microfibril disposition revealed by freeze-fractured plasmalemma not treated with glycerol. Planta 126, 93–96 (1975). https://doi.org/10.1007/BF00389363
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DOI: https://doi.org/10.1007/BF00389363