Summary
When the surface properties of etioplast and etiochloroplast membranes from dark grown or variously illuminated leaves of Avena sativa L. are examined by counter current distribution in a Dextran: poly(ethylene-glycol) two-phase system the results may be summarized as follows:
-
(i)
Etioplast membranes consist of two main classes with respect to upper phase poly(ethylene-glycol) affinity.
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(ii)
Shortly after illumination the proportion of higher affinity membranes increases and the upper phase affinity of this class also increases.
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(iii)
Further illumination reverses this effect producing a single class of membranes with the high affinity for the lower phase characteristic of membranes of the mature chloroplast.
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(iv)
In rapidly greening leaves (6–9 h illumination) and intermediate class is observed which may represent an intermediate stage in biogenesis.
The interpretation of these results is discussed in terms of changes in membrane surface reaction and potential.
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Abbreviations
- PEG:
-
poly(ethylene-glycol)
- TLCCD:
-
thin-layer counter current distribution apparatus
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Treffry, T. Developmental changes in the surface properties of chloroplast membranes. Planta 126, 11–17 (1975). https://doi.org/10.1007/BF00389355
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DOI: https://doi.org/10.1007/BF00389355