Summary
Dinactin, an antibiotic forming complexes with K+ ions, uncouples phosphorylation in chloroplasts without requiring the presence of a substance increasing the permeability of the membrane for protons. To inhibit photophosphorylation, less Dinactin is necessary in the absence than in the presence of K+.
When added before the light phase, Dinactin affects the light-triggered ATP-Pi exchange reaction in the same way as it does the complete photophosphorylation. Addition of the antibiotic after the activation by light inhibits the exchange reaction independently of the presence of K+, possibly by blocking the energy transfer to ATP.
The inhibition of the light-induced proton transport by Dinactin is more pronounced in the presence of K+ than of Na+ ions. The manner in which changes in the permeability of the chloroplast membrane for K+ ions caused by Dinactin may influence photophosphorylation and reactions coupled with it is discussed.
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Abbreviations
- ATP:
-
Adenosintriphosphat
- ADP:
-
Adenosindiphosphat
- Pa :
-
anorganisches Phosphat
- PMS:
-
Phenazinmethosulfat
- DCPIP:
-
Dichlorphenolindophenol
- FeCy:
-
Ferricyanid
- DNP:
-
Dinitrophenol
- FCCP:
-
Carbonylcyanid-p-trifluormethoxyphenylhydrazon
- SQ 15859:
-
Squibb Compound 15859
Literatur
Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenoloxydase in Beta vulgaris. Plant Physiol. 24, 1 (1949).
Avron, M.: Photophosphorylation by Swiss-chard chloroplasts. Biochim. biophys. Acta (Amst.) 40, 257 (1960).
Dilley, R. A., Shavit, N.: On the relationship of H+ transport to photophosphorylation in spinach chloroplasts. Biochim. biophys. Acta (Amst.) 162, 86 (1968).
—, Vernon, L. P.: Ion and water transport processes related to the light-dependant shrinkage of spinach chloroplasts. Arch. Biochem. 111, 365 (1965).
Dutcher, J. D.: Isolation and characterisation of a cytotoxic agent, SQ 15859, from Streptomyces chrysomallus. In: Antimicrobial agents and chemotherapy, p. 173 (M. Finland und G. M. Savage, ed.). Detroit: Am. Soc. Microbiology 1962.
Good, N., Izawa, S., Hind, G.: Uncoupling and energy transfer inhibition in photophosphorylation. In: Current topics in bioenergetics, vol. I, p. 76 (D. R. Sanadi, ed.). New York: Acad. Press 1966
Graven, S. N., Lardy, H. A., Estrada-O, S.: Antibiotics as tools for metabolic studies. VIII. Effect of nonactin homologs on alkali metal cation transport and rate of respiration in mitochondria. Biochemistry 6, 365 (1967).
——, Johnson, D., Rutter, A.: Antibiotics as tools for metabolic studies. V. Effect of nonactin, monactin, dinactin, trinactin on oxydative phosphorylation and ATPase induction. Biochemistry 5, 1729 (1966).
Karlish, S. J. D., Avron, M.: Dinitrophenol and valinomycin as uncouplers in isolated chloroplasts. FEBS Letters 1, 21 (1968).
Karlish, S. J. D., Shavit, N., Avron, M.: On the mechanism of uncoupling in chloroplasts by ionpermeability inducing agents. European J. Biochem. 9, 291 (1969).
Keller-Schierlein, W., Gerlach, H.: Makrotetrolide. Fortschr. Chem. org. Naturstoffe 26, 161 (1968).
Krogmann, D. W., Jagendorf, A. T.: A spectrophotometric assay of the Hill reaction with ferricyanide. Plant Physiol. 32, 373 (1957).
Lardy, H.: Influence of antibiotics and cyclic polyethers on ion transport in mitochondria. Fed. Proc. 27, 1278 (1968).
Molotkovsky, Y. G., Dzyubenko, V. S.: Formation of the pH gradient through membranes linked with energy dependant translocation of cations. Nature (Lond.) 219, 496 (1968).
Mueller, P., Rudin, D. O.: Development of K+-Na+ discrimination in experimental bimolecular lipid membranes by macrocyclic antibiotics. Biochem. biophys. Res. Commun. 26, 398 (1967).
Nobel, P. S.: Light-induced changes in the ionic content of chloroplasts in Pisum sativum. Biochim. biophys. Acta (Amst.) 172, 134 (1969).
Neumann, J., Jagendorf, A. T.: Light-induced pH changes related to phosphorylation. Arch. Biochim. 107, 109 (1964).
Packer, L.: Effect of nigericin upon light dependant monovalent cation transport in chloroplasts. Biochem. biophys. Res. Commun. 28, 1022 (1967).
—, Allen, J. M., Starks, M.: Light-induced ion transport in glutaraldehyde-fixed chloroplasts: studies with nigericin. Arch. Biochim. 128, 142 (1968).
Pioda, L. A. R., Wachter, H. A., Dohner, R. E., Simon, W.: Komplexe von Nonactin und Monactin mit Natrium- und Kalium- und Ammonium-Ionen. Helv. chim. Acta 50, 1375 (1967).
Plengvidhya, P., Burris, R. H.: Inhibitors of photophosphorylation and photoreduction. Plant Physiol. 40, 997 (1965).
Pressmann, B. C.: Ionophorous antibiotics as models for biological transport. Fed. Proc. 27, 1283 (1968).
Shavit, N., Dilley, R. A., San Pietro, A.: Ion translocation in isolated chloroplasts. Uncoupling of photophosphorylation and translocation of K+ and H+ Ions induced by nigericin. Biochemistry 7, 2356 (1968).
—, San Pietro, A.: K+ dependant uncoupling of photophosphorylation by Nigericin. Biochem. biophys. Res. Commun 28, 277 (1967).
Specht-Jürgensen, I., Bachofen, R.: Untersuchungen zur lichtinduzierten ATP-Pa-Austauschreaktion in Spinatchloroplasten. Planta (Berl.) 79, 222 (1968).
Stefanac, Z., Simon, W.: Ion specific electrochemical behaviour of macrotetrolides in membranes. Mikrochem. J. 12, 125 (1967).
Whatley, F. R., Arnon, D. I.: Photosynthetic phosphorylation in plants. In: Methods in enzymology, vol. VI, p. 308 (S. P. Colowick and N. O. Kaplan, ed.). New York: Academic Press 1963.
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Bachofen, R., Specht-Jürgensen, I. Die Wirkung von Dinactin auf die cyclische Photophosphorylierung, die lichtinduzierte ATP-Pa-Austauschreaktion und den lichtinduzierten Protonentransport in Chloroplasten. Planta 90, 66–79 (1969). https://doi.org/10.1007/BF00389297
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DOI: https://doi.org/10.1007/BF00389297