Marine Biology

, Volume 26, Issue 3, pp 249–260 | Cite as

Biochemistry and microbiology of some Irish Sea sediments: I. Amino-acid analyses

  • C. D. Litchfield
  • A. L. S. Munro
  • L. C. Massie
  • G. D. Floodgate


Cores of sedimentary mud, collected on a transect between the Isle of Man and Ireland, were qualitatively analysed for free amino acids by thin-layer chromatography of the dansylated (DNS) derivatives. The DNS derivatives of isoleucine/leucine and α-aminobutyric acid were found in all samples, while the derivatives of phenylalanine, valine, arginine and ammonia were usually present. Acid hydrolysis followed by treatment with ninhydrin showed the presence of peptidyl amino acids in half the DNS-reacted extracts. No distribution pattern of amino acids was found either with geographical location or down the depths of the cores. Attempts to quantify the method were unsuccessful. Other analyses indicated the presence of glucose/galactose, but no neutral or phospholipids or recognizable plant pigments were identified.


Hydrolysis Arginine Geographical Location Phenylalanine Valine 
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Literature Cited

  1. Belderson, R.H.: Holocene sedimentation in the western half of the Irish Sea. Mar. Geol. 2, 147–163 (1964)CrossRefGoogle Scholar
  2. Bohling, H.: Untersuchungen über freie gelöste Aminosäuren in Meerwasser. Mar. Biol. 6, 213–225 (1970)Google Scholar
  3. Button, D.L.: Effect of clay on the availability of dilute organic nutrients to steady-state populations. Limnol. Oceanogr. 14, 94–100 (1970)Google Scholar
  4. Chau, Y.K. and J.P. Riley: The determination of amino acids in sea water. Deep Sea Res. 13, 1115–1124 (1966)Google Scholar
  5. Degens, E.T., J.H. Reuter and K.N.F. Shaw: Biochemical compounds in offshore California sediments and sea water. Geochim. cosmochim. Acta 28, 45–66 (1964)CrossRefGoogle Scholar
  6. De Stafanis, V.A. and J.G. Ponte, Jr.: Separation of sugars by thin-layer chromatography. J. Chromat. 34, 116–120 (1968)CrossRefGoogle Scholar
  7. Erdmann, J.G., E.M. Marlett and W.E. Hanson: Survival of amino acids in marine sediments. Science, N.Y. 124, p. 1026 (1956)Google Scholar
  8. Folin, O. and H. Malmros: An improved form in Folin's micromethod for blood sugar determinations. J. biol. Chem. 83, 115–120 (1929)Google Scholar
  9. Garside, C. and J.P. Riley: A thin-layer chromatographic method for the determination of plant pigments in sea water and cultures. Analutica chim. Acta. 46, 179–191 (1969)CrossRefGoogle Scholar
  10. Gray, W.R.: Dansyl chloride procedure. In: Methods of enzymology, Vol. 11. Enzyme structure. pp 139–150. Ed. by C.H.W. Hirs. New York: Academic Press 1967Google Scholar
  11. Hamilton, R.D. and L.J. Greenfield: The utilization of free amino acids by marine sediment microbionts. Z. allg. Mikrobiol. 7, 335–342 (1967)PubMedGoogle Scholar
  12. Handa, N.: Examination on the applicability of the phenol sulfuric acid method on the determination of dissolved carbohydrate in sea water. J. oceanogr. Soc. Japan 22(3), 81–86 (1966)Google Scholar
  13. — and H. Tominaga: A detailed analysis of carbohydrates in marine particulate matter. Mar. Biol. 2, 228–235 (1969)Google Scholar
  14. Hirs, C.H.W.: Detection of peptides by chemical methods. In: Methods in enzymology. Vol. 11. Enzyme structure, pp 325–330. Ed. by C.H.W. Hirs. New York: Academic Press 1967Google Scholar
  15. Hobbie, J.E., C.C. Crawford, and K.L. Webb: Amino acid flux in an estuary. Science, N.Y. 159, 1463–1464 (1968)Google Scholar
  16. Itihara, Y.: Amino acids in the recent sediments. J. geol. Soc. Japan 73, 247–254 (1967)Google Scholar
  17. — and M. Itihara: Amino acids in muddy sediments on the Osaka Group. J. geol. Soc. Japan 74, 233–238 (1968)Google Scholar
  18. Litchfield, C.D. and J.M. Prescott: Analysis by dansylation of amino acids dissolved in marine and fresh waters. Limnol. Oceanogr. 15, 250–256 (1970)Google Scholar
  19. Plunkett, M.A.: The qualitative determination of some organic compounds in marine sediments. Deep Sea Res. 5, 259–262 (1957)CrossRefGoogle Scholar
  20. Rashid, M.A. and L.H. King: Chemical characteristics of fractionated humic acids associated with marine sediments. Chem. Geol. 7, 37–43 (1971)CrossRefGoogle Scholar
  21. Rittenberg, S.C., K.O. Emery, J. Hulsemann, E.T. Degens, R.C. Fay, J.H. Reuter, J.R. Grady, S.H. Richardson, and E.E. Bray: Bio-geochemistry of sediments in experimental Mohole. J. sedim. Petrol. 33, 140–172 (1963)Google Scholar
  22. Stahl, E.: Thin-layer chromatography, 1041 pp. New York: Springer-Verlag 1969Google Scholar
  23. Stanley, S.O. and S.M. Brown: Effect of changes in temperature and salinity on the intracellular amino acid pool composition of some marine bacteria. In: Effect of the ocean environment on microbial activities, 2nd U.S.-Japan Conference on Marine Microbiology. Ed. by R.R. Colwell and R.Y. Morita 1974Google Scholar
  24. Tatsumato, M., W.T. Williams, J.M. Prescott and D.W. Hood: Amino acids in samples of surface sea water. J. mar. Res. 19, 89–96 (1961)Google Scholar
  25. Waksman, S.A.: On the distribution of organic matter in the sea bottom and the chemical nature and origin of marine humus. Soil Sci. 36, 125–147 (1933)Google Scholar
  26. Wehmiller, J. and P.E. Hare: Racemization of amino acids in marine sediments. Science, N.Y. 173, 907–911 (1971)Google Scholar
  27. West, E.S., W.R. Todd, H.S. Mason and J.T. Van Bruggen: Textbook of biochemistry, 4th ed. 1595 pp. New York: Macmillan Co. 1966Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • C. D. Litchfield
    • 1
    • 3
    • 2
  • A. L. S. Munro
    • 1
    • 2
  • L. C. Massie
    • 1
    • 2
  • G. D. Floodgate
    • 1
    • 2
  1. 1.Marine Science LaboratoriesUniversity College of North WalesMenai BridgeUK
  2. 2.Marine LaboratoryAberdeenScotland, UK
  3. 3.Department of BacteriologyRutgers UniversityNew BrunswickUSA

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