Summary
Microautoradiographic techniques were used to determine the distribution of Ca45 and S35 in regions of the bean root where anatomical features may influence the processes of ion uptake and translocation. Root tissue from intact plants was prepared by methods that preserve both soluble and insoluble Ca and S. Ca45 distribution was determined after 1 hour and 15 min, of uptake, after 2 efflux periods, and after replacement by non-tracer Ca.
S35 distribution was determined after 1 hour and 15 min of uptake.
The quantity of Ca45 that entered the root was greater than the quantity of S35. Ca45 concentration within the root increased with linear distance from the 8-mm level behind the tip. The pathways of Ca and S across the cortex appeared to be different since Ca45 was particularly associated with cell walls and S35 was distributed more evenly through the cells. There was no evidence that the endodermis was a diffusion barrier for Ca; the small parenchyma cells associated with conducting elements acquired a high concentration of Ca45 and thus appear to be implicated in absorption and perhaps in transfer to the xylem. The evidence suggests that the endodermis may have been a barrier for S, but if so, certain parenchyma cells inside the stele, especially at xylem poles, were equally involved. The region from 30 to 80 mm from the tip appeared to participate in Ca uptake and transfer to the xylem; because of tissue immaturity the 8-mm region, which contained the least Ca45, was thought not to translocate to the shoot. Deposition of Ca45 in oxalate crystals represented almost complete immobilization. Calcium oxalate metabolism was most active in the 30-mm region of secondary roots and in their small branches. S35-labelled nuclei occurred in the cortex 2.5 to 3 mm behind the root tip.
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References
Arisz, W. H.: Significance of the symplasm theory for transport across the root. Protoplasma (Wien) 46, 5–62 (1956).
Bell, C. W.: Calcium movement and deposition in the stem of the bean plant. Ph.D. Diss., Washington State Univ. 1963.
Bell, C. W., and O. Biddulph: Translocation of calcium. Exchange versus mass flow. Plant Physiol. 38, 610–614 (1963).
Biddulph, O., F. S. Nakayama, and R. Cory: Transpiration stream and ascension of calcium. Plant Physiol. 36, 429–436 (1961).
Biddulph, S. F.: Visual indications of S35 and P32 translocation in the phloem. Amer. J. Bot. 43, 143–148 (1956).
Branton, D., and L. Jacobson: Iron localization in pea plants. Plant Physiol. 37, 546–551 (1962a).
—: Dry, high resolution autoradiography. Stain Technol. 37, 239–242 (1962b).
Brouwer, R.: The regulating influence of transpiration and suction tension on the water and salt uptake by the root of intact Vicia faba plants. Acta bot. neerl. 3, 264–312 (1954).
Canning, R. E., and P. J. Kramer: Salt accumulation in various regions of roots. Amer. J. Bot. 45, 378–382 (1958).
Crafts, A. S., and T. C. Broyer: Migration of salts and water into xylem of the roots of higher plants. Amer. J. Bot. 25, 529–535 (1938).
Epstein, E.: Passive permeation and active transport of ions in plant roots. Plant Physiol. 30, 529–535 (1955).
Evans, E. C. III: Polar transport of calcium in the primary root of Zea mays. Science 138, 174–177 (1964).
Howard, A., and S. P. Pelc: Synthesis of nucleoprotein in bean root cells. Nature (Lond.) 167, 599–600 (1951a).
—: Nuclear incorporation of P32 as demonstrated by autoradiographs. Exp. Cell Res. 2, 178–187 (1951b).
Jansen, E. F., R. Jang, P. Albersheim, and J. Bonner: Pectic metabolism of growing cell walls. Plant Physiol. 35, 87–97 (1960).
Laties, G. G.: Active transport of salt into plant tissue. Ann. Rev. Plant Physiol. 10, 87–112 (1959).
Lüttge, U., u. J. Weigl: Mikroautoradiographische Untersuchungen der Aufnahme und des Transportes von 35SO4 and 45Ca in Keimwurzeln von Zea mays L. Planta (Berl.) 58, 113–126 (1962).
Lundegårdh, H.: Anion respiration. The experimental basis of a theory of absorption, transport and exudation of electrolytes by living cells and tissues. Symp. Soc. exp. Biol. 8, 262–292 (1954).
Pate, J. S.: Roots as organs of assimilation of sulphate Science 149, 547–548 (1965).
Portyanko, V. F.: The stimulating role of the root apex in absorption of nutrients. Fiziol. Rasteniî 11, 170–173 (1964) (Translation).
Priestley, J. H.: The mechanism of root pressure. New Phytol. 19, 189–200 (1920).
Russell, R. S., and D. A. Barber: The relationship between salt uptake and the absorption of water by intact plants. Ann. Rev. Plant Physiol. 11, 127–140 (1960).
Scott, F. M.: Root hair zone of soil grown roots. Nature (Lond.) 199, 1009–1010 (1963).
Steward, F. C., and J. F. Sutcliffe: Plants in relation to inorganic salts. In: F. C. Steward (ed.), A treatise of plant physiology, vol. II, pp. 253–478. New York and London: Academic Press 1959.
Sutcliffe, J. F.: Mineral salts absorption in plants. New York: Macmillan 1962.
Van Fleet, D. S.: Histochemistry and function of the endodermis. Bot. Rev. 27, 165–220 (1961).
Weigl, J., u. U. Lüttge Mikroautoradiographische Untersuchungen über die Aufnahme von 35SO4 durch Wurzeln von Zea mays L. Die Funktion der primären Endodermis. Planta (Berl.) 59, 15–28 (1962).
Wiebe, H. H., and P. J. Kramer: Translocation of radioactive isotopes from various regions of roots of barley seedlings. Plant Physiol. 29, 342–348 (1954).
Wiersum, L. K.: Transfer of solutes across the young root Rec. Trav. bot. néerl. 41, 1–79 (1947).
Williams, B. C.: The structure of the meristematic root tip and origin of the primary tissue in the roots of vascular plants. Amer. J. Bot. 34, 455–462 (1947).
Williams, D. E.: Anion-exchange properties of plant root surfaces. Science 138, 153–154 (1962).
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Biddulph, S.F. A microautoradiographic study of Ca45 and S35 distribution in the intact bean root. Planta 74, 350–367 (1967). https://doi.org/10.1007/BF00389093
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DOI: https://doi.org/10.1007/BF00389093