Summary
Thin-layer electrophoresis in conjunction with thin-layer chromatography proved an excellent method for separating gibberellin-like compounds extracted from small quantities of peach tissue. Eleven compounds active in the barley-endosperm gibberellin bioassay were separated from peach shoot tips. Two of these were possibly identical with known gibberellins but the remainder were distinctly different. Of the latter, five were acidic, one was neutral, and three were basic. The most active compound in shoot tips may have been GA1 or GA3 but in seeds the most active compound resembled no known gibberellin.
Similar content being viewed by others
References
Bieleski, R. L., and N. A. Turner: Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Analyt. Biochem. 17, 278–293 (1966).
Bollard, E. G.: The use of tracheal sap in the study of apple tree nutrition. J. exp. Bot. 4, 363–368 (1953).
Briggs, D. E.: Residues from organic solvents showing gibberellin-like biological activity. Nature (Lond.) 210, 419–421 (1966).
Coombe, B. G., D. Cohen, and L. G. Paleg: Development of the barley endosperm bioassay for gibberellin. III. Application of the method. Plant Physiol. (in press).
Hashimoto, T., and L. Rappaport: Variations in endogenous gibberellins in developing bean seeds. I. Occurrence of neutral and acidic substances. Plant Physiol. 41, 623–628 (1966).
Hayashi, F., and L. Rappaport: Gibberellin-like activity of neutral and acidic substances in the potato tuber. Nature (Lond.) 195, 617–618 (1962).
Jackson, D. I., and B. G. Coombe: Gibberellin-like substances in the developing apricot fruit. Science 154, 277–278 (1966).
Ogawa, Y.: Acid, neutral and “water soluble” gibberellin-like substances occurring in developing seed of Sechium edule. Bot. Mag. (Tokyo) 79, 1–6 (1966a).
—: Ethyl acetate-soluble and “water-soluble” gibberellin-like substances in the seeds of Pharbitis nil, Lupinus luteus and Prunus persica. Bot. Mag. (Tokyo) 79, 69–76 (1966b).
Paleg, L. G.: Physiological effects of gibberellins. Ann. Rev. Plant Physiol. 16, 291–322 (1965).
—, D. Aspinall, B. Coombe, and P. Nicholls: Physiological effects of gibberellic acid. VI. Other gibberellins in three test systems. Plant Physiol. 39, 286–290 (1964).
Radley, M.: The distribution of substances similar to gibberellic acid in higher plants. Ann. Bot. N.S. 22, 297–307 (1958).
Schneider, G., G. Sembdner u. K. Schreiber: Gibberelline. VI. Die dünnschichtelektrophorese von Gibberellinen. J. Chromatog. 19, 358–363 (1965).
Van Overbeek, J.: Plant hormones and regulators. Science 152, 721–731 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jackson, D.I. Gibberellin-like substances in peach tissue: Separation by thin-layer electrophoresis and chromatography. Planta 74, 324–329 (1967). https://doi.org/10.1007/BF00389091
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00389091