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A simple technique for obtaining functionally isolated guard cells in epidermal strips of Vicia faba

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Summary

Ultrasonic irradiation of Vicia faba L. epidermal strips for 2 min preferentially disrupts the epidermal cells but does not impair guard cell movements. Maximal opening induced by fusicoccin requires that K+ be provided to the guard cells from external sources. A mobile organic anion is not required.

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Author notes

  1. Richard D. Durbin

    Present address: ARS, USDA, Department of Plant Pathology, University of Wisconsin, 53706, Madison, Wis., USA

Authors and Affiliations

  1. Centro di studio del CNR su le tossine e i parassiti sistemici dei vegetali, Istituto di Patologia vegetale, Università, Via G. Amendola 165/A, 70126, Bari, Italy

    Richard D. Durbin & Antonio Graniti

Authors
  1. Richard D. Durbin
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  2. Antonio Graniti
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Durbin, R.D., Graniti, A. A simple technique for obtaining functionally isolated guard cells in epidermal strips of Vicia faba . Planta 126, 285–288 (1975). https://doi.org/10.1007/BF00388970

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  • DOI: https://doi.org/10.1007/BF00388970

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