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Light-induced structural changes during incubation of isolated maize etioplasts

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Summary

Etioplasts were isolated from leaves of 9-day-old etiolated maize (Zea mays L.) seedlings and incubated in a relatively simple medium in light and in the dark. During the first 5 h no changes occurred in the fine structure of the isolated etioplasts in the dark. In light the size of the prolamellar bodies decreased and significantly more plastid sections without prolamellar bodies were counted. The total length of the thylakoids per plastid section increased, but there was no evidence for bi- and polythylakoid formation. It is concluded that light induces the structural transformation of the prolamellar body membranes into primary thylakoids also in isolated etioplasts.

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References

  • Anderson, J.M., Boardman, N.K.: Studies on the greening of darkgrown bean plants. II. Development of photochemical activity. Aust. J. biol. Sci. 17, 93–101 (1964)

    Google Scholar 

  • Bogorad, L., Falk, R.H., Forger, J.M., III, Lockshin, A.: Properties of etioplast membranes and membrane development in maize. In: Anatomy and biogenesis of mitochondria and chloroplasts, pp. 85–91, Eds.: Boardman, N.K., Linnane, A.W., Smillie, R.M.: Amsterdam-London: North Holland 1971

    Google Scholar 

  • Cockburn, W., Walker, D.A., Baldry, C.W.: The isolation of spinach chloroplasts in pyrophosphate media. Plant Physiol. 43, 1415–1418 (1968)

    Google Scholar 

  • Cockburn, B.J., Wellburn, A.R.: Changes in the envelope permeability of developing chloroplasts. J. exp. Bot. 25, 36–49 (1974)

    Google Scholar 

  • Eriksson, G., Kahn, A., Walles, B., von Wettstein, D.: Zur makromolekularen Physiologie der Chloroplasten. III.. Ber. dtsch. bot. Ges. 74, 221–232 (1961)

    Google Scholar 

  • Grosse, W., Eickhoff, F.: Localization of tryptophan synthetase in etioplasts of Pisum sativum L. Planta (Berl.) 118, 25–34 (1974)

    Google Scholar 

  • Hearing, V.J.: Protein synthesis in isolated etioplasts after light stimulation. Phytochem. 12, 277–282 (1973)

    Article  Google Scholar 

  • Henningsen, K.W., Boynton, J.E.: Macromolecular physiology of plastids. VII. The effect of a brief illumination on plastids of dark-grown barley leaves. J. Cell Sci. 5, 757–793 (1969)

    PubMed  Google Scholar 

  • Horton, P., Leech, R.M.: The effect of ATP on the photoconversion of protochlorophyllide in isolated etioplasts of Zea mays. Plant Physiol. 56, 113–120 (1975)

    Google Scholar 

  • Jacobson, A.B.: A procedure for isolation of proplastids from etiolated maize leaves. J. Cell Biol. 38, 238–244 (1968)

    Article  PubMed  Google Scholar 

  • Kahn, A.: Developmental physiology of bean leaf plastids. II. Negative contrast electron microscopy of tubular membranes in prolamellar bodies. Plant Physiol. 43, 1769–1780 (1968)

    Google Scholar 

  • Kirk, J.T.O., Tilney-Bassett, R.A.E.: The Plastids. London-San Fransisco: Freeman 1967

    Google Scholar 

  • Klein, S., Harel, E., Ne'eman, E., Katz, E., Meller, E.: Accumulation of δ-aminolaevulinic acid and its relation to chlorophyll synthesis and development of plastid structure in greening leaves. Plant Physiol. 56, 486–496 (1975)

    PubMed  Google Scholar 

  • Klein, S., Poljakoff-Mayber, A.: Isolation of proplastids from etiolated bean leaves. Exp. Cell Res. 24, 143–200 (1961a)

    PubMed  Google Scholar 

  • Klein, S., Poljakoff-Mayber, A.: Fine structure and pigment conversion in isolated proplastids. J. biophys. biochem. Cytol. 2, 433–440 (1961b)

    Google Scholar 

  • Luft, J.H.: Improvements in epoxyresine embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Article  PubMed  Google Scholar 

  • MacKinney, G.: Absorption of light by chlorophyll solutions. J. biol. Chem. 140, 315–322 (1941)

    Google Scholar 

  • Rebeiz, C.A., Mattheis, J.R., Smith, B.: The net synthesis of protochlorophyll from δ-aminolevulinic acid by isolated plastids. (Abstr.) Plant Physiol. 56 (2), Suppl., 31 (1975)

    Google Scholar 

  • Reger, B.J., Smillie, R.M., Fuller, R.C.: Protein synthesis by isolated etioplasts and chloroplasts from pea and wheat and the effects of chloramphenicol and cycloheximide. Plant Physiol. 50, 19–23 (1972)

    Google Scholar 

  • Wellburn, F.A.M., Wellburn, A.R.: Chlorophyll synthesis by isolated intact etioplasts. Biochem. biophys. Res. Commun. 45, 747–750 (1971a)

    PubMed  Google Scholar 

  • Wellburn, F.A.M., Wellburn, A.R.: Developmental changes occurring in isolated intact etioplasts. J. Cell Sci. 9, 271–287 (1971b)

    PubMed  Google Scholar 

  • Wellburn, A.R., Wellburn, F.A.M.: Developmental changes of etioplasts in isolated suspensions and in situ. Ann. Bot. 37, 11–19 (1973)

    Google Scholar 

  • Wrischer, M.: Ultrastructural changes in isolated plastids. I. Etioplasts. Protoplasma 78, 291–303 (1973a)

    Google Scholar 

  • Wrischer, M.: Ultrastructural changes in isolated plastids. II. Etiochloroplasts. Protoplasma 78, 417–425 (1973b)

    Google Scholar 

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Kohn, S., Klein, S. Light-induced structural changes during incubation of isolated maize etioplasts. Planta 132, 169–175 (1976). https://doi.org/10.1007/BF00388899

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  • DOI: https://doi.org/10.1007/BF00388899

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