Skip to main content
SpringerLink
Log in

Experiments on enzymatic acylation of sn-glycerol 3-phosphate with enzyme preparations from pea and spinach leaves

  • Published:
Planta Aims and scope Submit manuscript

Summary

Optimal reaction conditions were investigated for acylation of sn-[U-14C]glycerol 3-phosphate in cell-free systems from leaves of Pisum sativum L. and Spinacia oleracea L. With palmitoyl-CoA as acyl donor the major product formed was monoacyl glycerol phosphate. In pea seedlings enzymatic activity was found to be dependent on the age of shoots going through a maximum 12 days after planting. In such plants the highest enzymatic activity is found in leaves and not in the shoot apex. Also in leaves activity varies according to the age of these organs. In leaves of maximal activity the highest total and specific activity was found in soluble proteins from chloroplasts. In older pea and spinach leaves higher activities were observed in membranes from chloroplasts and microsomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barden, R.E., Cleland, W.W.: I-Acylglycerol 3-phosphate acyltransferase from rat liver. J. Biol. Chem. 244, 3677–3684 (1969)

    PubMed  Google Scholar 

  • Barron, E.J., Stumpf, P.K.: Fat metabolism in higher plants XIX. The biosynthesis of triglycerides by a vocado-mesocarp enzymes. Biochim. Biophys. Acta 60, 329–337 (1962)

    Article  Google Scholar 

  • Bergmeyer, H.U.: Methoden der enzymatischen Analyse, 2. Aufl., Weinheim: Verlag Chemie 1970

    Google Scholar 

  • Berset, C., Etemadi, A.H.: Acyl-CoA-ACP-transacylases of Mycobacterium smegmatis. An analytical study. Biochimie 55, 1381–1394 (1973)

    PubMed  Google Scholar 

  • Bjerve, K.S., Daae, L.N.W., Bremer, J.: The selective loss of lysophospholipids in some commonly used lipid-extraction procedures. Analyt. Biochem. 58, 238–245 (1974)

    PubMed  Google Scholar 

  • Bligh, E.G., Dyer, W.J.: A rapid method of total lipid extraction and purification. Canad. J. Biochem. Physiol. 37, 911–917 (1959)

    Google Scholar 

  • Börner, T.: Struktur und Funktion der genetischen Information in den Plastiden. Biol. Zentralbl. 92, 545–561 (1973)

    Google Scholar 

  • Bruinsma, J.: A comment on the spectrophotometric determination of chlorophyll. Biochim. Biophys. Acta 52, 576–578 (1961)

    Article  PubMed  Google Scholar 

  • Cheniae, G.M.: Phosphatidic acid and glyceride synthesis by particles from spinach leaves. Plant Physiol. 40, 235–243 (1965)

    PubMed  Google Scholar 

  • Cooper, T.G.: The activation of fatty acids in castor bean endosperm. J. Biol. Chem. 246, 3451–3455 (1971)

    PubMed  Google Scholar 

  • Dittmer, J.C., Lester, R.L.: A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J. Lipid Res. 5, 126–127 (1964)

    Google Scholar 

  • Feige, B., Gimmler, H., Jeschke, W.D., Simonis, W.: Eine Methode zur dünnschichtchromatographischen Auftrennung von 14C-und 32P-markierten Stoffwechselprodukten. J. Chromatogr. 41, 80–90 (1969)

    Article  PubMed  Google Scholar 

  • Folch, J., Lees, M., Stanley, G.H.S.: A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497–509 (1957)

    PubMed  Google Scholar 

  • Galliard, T.: A simple biochemical method for the preparation of high specific activity [14C]acyl-labelled phosphatidylcholine and other phospholipids. Biochim. Biophys. Acta 260, 541–546 (1972)

    PubMed  Google Scholar 

  • Hajra, A.K.: On extraction of acyl and alkyl dihydroxyacetone phosphate from incubation mixtures. Lipids 9, 502–505 (1974)

    PubMed  Google Scholar 

  • Hanes, C.S., Isherwood, F.A.: Separation of the phosphoric esters on the filter paper chromatogram. Nature 164, 1107–1112 (1949)

    PubMed  Google Scholar 

  • Hauser, G., Eichberg, J.: Identification of cytidine diphosphate-diglyceride in the pineal gland of the rat and its accumulation in the presence of DL-propanolol. J. Biol. Chem. 250, 105–112 (1975)

    PubMed  Google Scholar 

  • Jamdar, S.C., Fallon, H.J.: Glycerolipid biosynthesis in rat adipose tissue I. Properties and distribution of glycerophosphate acyltransferase and effect of divalent cations on neutral lipid formation. J. Lipid Res. 14, 509–516 (1973)

    PubMed  Google Scholar 

  • Jaworski, J.G., Goldschmidt, E.E., Stumpf, P.K.: Fat metabolism in higher plants. Properties of the palmityl acyl carrier protein: stearly acyl carrier protein elongation system in maturing safflower seed extracts. Arch. Biochem. Biophys. 163, 769–776 (1974)

    PubMed  Google Scholar 

  • Jaworski, J.G., Stumpf, P.K.: Fat metabolism in higher plants. Enzymatic preparation of E. coli stearoyl-acyl carrier protein. Arch. Biochem. Biophys. 162, 166–173 (1974)

    PubMed  Google Scholar 

  • Kluytmans, J.H., Raju, P.K.: Partial purification and some properties of stearoyl-CoA: sn-glycerol-3-phosphate acyltransferase from liver microsomes. Prep. Biochem. 4, 141–163 (1974)

    PubMed  Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)

    PubMed  Google Scholar 

  • Lueking, D., Tokuhisa, D., Sojka, G.: Glycerol assimilation by a mutant of Rhodopseudomonas capsulata. J. Bacteriol. 115, 897–903 (1973)

    PubMed  Google Scholar 

  • Macher, B.A., Brown, C.P., McManus, T.T., Mudd, J.B.: Studies on phospholipid-synthesizing enzyme activities during the growth of etiolated cucumber cotyledons. Plant Physiol. 55, 130–136 (1975)

    Google Scholar 

  • Miflin, B.J., Beevers, H.: Isolation of intact plastids from a range of plant tissues. Plant Physiol. 53, 870–874 (1974)

    Google Scholar 

  • Moore, R.E., Springer-Lederer, H., Ottenheym, H.C., Bassham, J.A.: Photosynthesis by isolated chloroplasts IV. Regulation by factors from leaf cells. Biochim. Biophys. Acta 180, 368–376 (1969)

    PubMed  Google Scholar 

  • Morré, D.J.: Membrane biogenesis. Ann. Rev. Plant Physiol. 26, 441–481 (1975)

    Article  Google Scholar 

  • Mudd, J.B., Garcia, R.E.: Biosynthesis of glycolipids. In: Recent advances in the chemistry and biochemistry of plant lipids, pp. 161–201. Ed.: Galliard, T., Mercer, E.J., London-New York: Academic Press 1975

    Google Scholar 

  • Rekonen, O., Bloch, K.: Biosynthesis of monogalactosyldiglycerides in photoauxotrophic Euglena gracilis. J. Biol. Chem. 244, 4899–4903 (1969)

    PubMed  Google Scholar 

  • Rouser, G., Kritchevsky, G., Yamamoto, A., Simon, G., Galli, C., Bauman, A.J.: DEAE and TEAE cellulose column chromatographic procedures for phospholipids, glycolipids, and pigments. In: Methods in enzymology, Vol. 14, pp. 272–317. Ed.: Lowenstein, J.M., New York-London: Academic Press 1969

    Google Scholar 

  • Sastry, P.S., Kates, M.: Biosynthesis of lipids in plants II. Incorporation of glycerophosphate-32P into phosphatides by cell-free preparations from spinach leaves. Canad. J. Biochem. 44, 459–467 (1966)

    Google Scholar 

  • van den Bosch, H.: Phosphoglyceride metabolism. Ann. Rev. Biochem. 43, 243–277 (1974)

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000, Köln, Federal Republic of Germany

    M. Bertrams & E. Heinz

Authors
  1. M. Bertrams
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Heinz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bertrams, M., Heinz, E. Experiments on enzymatic acylation of sn-glycerol 3-phosphate with enzyme preparations from pea and spinach leaves. Planta 132, 161–168 (1976). https://doi.org/10.1007/BF00388898

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388898

Keywords

Search

Navigation