Summary
The distribution of glyoxylate-cycle enzymes between microbodies and mitochondria was examined in ethanol-grown Aspergillus tamarii Kita. Particulate activities of catalase and the two glyoxylate by-pass enzymes, malate synthase and isocitrate lyase, were localized in the microbodies. The microbodies had a buoyant density of about 1.23 g cm-3 after isopycnic centrifugation in linear sucrose gradients. Particulate activities of the other two glyoxycitrate synthase, together with that of succinate dehydrogenase were restricted to the mitochondria, which had a buoyant density of about 1.20 g cm-3. Catalase also appeared to be localized in a second particle, perhaps the microbody inclusions or the Woronin bodies, having a buoyant density of about 1.26 g cm-3.
Similar content being viewed by others
References
Beevers, H.: Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann. N.Y. Acad. Sci. 168, 313–324 (1969)
Burke, J.J., Trelease, R.N.: Cytochemical demonstration of malate synthase and glycolate oxidase in microbodies of cucumber cotyledons. Plant Physiol. 56, 710–717 (1975)
Camp, R.R., Whittingham, W.F.: Host-parasite relationships in sooty blotch disease of white clover. Amer. J. Bot. 59, 1057–1067 (1972)
Cooper, T.G., Beevers, H.: Mitochondria and glyoxysomes from castor bean endosperm. J. biol. Chem. 244, 3507–3513 (1969)
Flavell, R.B., Fincham, J.R.S.: Acetate-nonutilizing mutants of Neurospora crassa. II. Biochemical deficiencies and the role of certain enzymes. J. Bact. 95, 1063–1068 (1968)
Flavell, R.B., Woodward, D.O.: Metabolic role, regulation of synthesis, cellular localization, and genetic control of the glyoxylate cycle enzymes in Neurospora crassa. J. Bact. 105, 200–210 (1971)
Frederick, S.E., Newcomb, E.H.: Cytochemical localization of catalase in leaf microbodies (peroxisomes) J. Cell Biol. 43, 343–353 (1969)
Frederick, S.E., Newcomb, E.H.: Ultrastructure and distribution of microbodies in leaves of grasses with and without CO2-photorespiration. Planta (Berl.) 96, 152–174 (1971)
Graves, L.B., Jr., Trelease, R.N., Grill, A., Becker, W.M.: Localization of glyoxylate cycle enzymes in glyoxysomes in Euglena. J. Protozool. 19, 527–532 (1972)
Grove, S.N., Bracker, C.E., Morré, D.J.: An ultrastructural basis for hyphal tip growth in Pythium ultimum. Amer. J. Bot. 57, 245–266 (1970)
Hänssler, G., Maxwell, D.P., Maxwell, M.D.: Demonstration of acid phosphatase-containing vacuoles in hyphal tip cells of Sclerotium rolfsii. J. Bact. 124, 997–1006 (1975)
Kobr, M.J., Vanderhaeghe, F.: Changes in density of organelles from Neurospora. Experientia 29, 1221–1223 (1973)
Kobr, M.J., Vanderhaeghe, F., Combépine, G.: Particulate enzymes of the glyoxylate cycle in Neurospora crassa. Biochem. Biophys. Res. Commun. 37, 640–645 (1969)
Laborda, F., Maxwell, D.P.: Ultrastructural changes in Cladosporium cucumerinum during pathogenesis. Canad. J. Microbiol. 22, 394–403 (1976)
Lowry, O.H., Rosebrough, N.J., Farr, A.J., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Lück, H.: Catalase. In: Methods of enzymatic analysis. pp. 885–894, Ed.: Bergemeyer, H.U. New York: Academic Press 1963
Maxwell, D.P., Lumsden, R.D.: Oxalic acid production by Sclerotinia sclerotiorum in infected bean and in culture. Phytopathology 60, 1395–1398 (1970)
Maxwell, D.P., Maxwell, M.D., Hänssler, G., Armentrout, V.N., Murray, G.M., Hoch, H.C.: Microbodies and glyoxylate-cycle enzyme activities in filamentous fungi. Planta (Berl.) 124, 109–123 (1975)
Maxwell, D.P., Williams, P.H., Maxwell, M.D.: Studies on the possible relationships of microbodies and multivesicular bodies to oxalate, endopolygalacturonase, and cellulase (Cx) production by Sclerotinia sclerotiorum. Canad. J. Bot. 50, 1743–1748 (1972)
Mills, G.L., Cantino, E.C.: Isolation and characterization of microbodies and symphyomicrobodies with different buoyant densities from the fungus Blastocladiella emersonii. Biochem. Biophys. Res. Commun. 67, 1256–1263 (1975)
Müller, M., Hogg, J.F., De Duve, C.: Distribution of tricarboxylic acid cycle enzymes and glyoxylate cycle enzymes between mitochondria and peroxisomes in Tetrahymena pyriformis. J. biol. Chem. 243, 5385–5395 (1968)
O'Sullivan, J., Casselton, P.J.: The subcellular localization of glyoxylate cycle enzymes in Coprinus lagopus (sensu Buller). J. Gen. Microbiol. 75, 333–337 (1973)
Parish, R.W.: The lysosome-concept in plants. II. Localization of acid hydrolases in maize root tips. Planta (Berl.) 123, 15–31 (1975)
Roggenkamp, R., Sahm, H., Hinkelmann, W., Wagner, F.: Alcohol oxidase and catalase in peroxisomes of methanol-grown Candida boidinii. Euron. J. Biochem. 59, 231–236 (1975)
Szabo, A.S., Avers, C.J.: Some aspects of regulation of peroxisomes and mitochondria in yeast. Ann. N.Y. Acad. Sci. 168, 302–312 (1969)
Tolbert, N.E.: Compartmentation and control in microbodies. Symp. Soc. exp. Biol. 27, 215–239 (1973)
Trelease, R.N., Becker, W.M., Gruber, P.J., Newcomb, E.H.: Microbodies (glyoxysomes and peroxisomes) in cucumber cytyledons. Plant Physiol. 48, 461–475 (1971)
Wergin, W.P.: Ultrastructural comparison of microbodies in pathogenic and saprophytic hyphae of Fusarium oxysporum f. sp. lycopersici. Phytopathology 62, 1045–1051 (1972)
Wergin, W.P.: Development of Woronin bodies from microbodies in Fusarium oxysporum f. sp. lycopersici. Protoplasma 76, 249–260 (1973)
Wilson, C.L., Stiers, D.L., Smith, G.G.: Fungal lysosomes or spherosomes. Phytopathology 60, 216–227 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Graves, L.B., Armentrout, V.N. & Maxwell, D.P. Distribution of glyoxylate-cycle enzymes between microbodies and mitochondria in Aspergillus tamarii . Planta 132, 143–148 (1976). https://doi.org/10.1007/BF00388895
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388895