Summary
The effect of the nature and concentration of the nitrogen source on respiratory activity and removal of carbohydrate from the medium in suspension cultured sycamore (Acer pseudoplatanus L.) cells was determined. Comparison was also made of the rates of uptake of the two alternative nitrogen sources, nitrate and glutamate, at differing initial nitrogen concentrations within the range 7–14 mM. The initial pH of the culture medium before inoculation was 5.2; after inoculation the pH of both nitrate and glutamate cultures rose to reach an eventual level in the range 7.0–7.1. Glutamate was removed from the medium more slowly than nitrate. Under the particular conditions of culture used the growth of the cells was nitrogen limited. Sugar uptake from the medium continued for some time after the nitrogen in the medium was depleted. The data show that although cell division and protein content are nitrogen-limited, dry weight and fresh weight yields may also be determined in a complex interaction through carbohydrate availability. There were no obvious differences in respiratory activity between cultures grown on nitrate or glutamate.
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Bernt, E., Bergmeyer, H.N.: L-Glutamate UV assay with glutamate dehydrogenase and NAD. In: Methods of enzymic analysis, 4, 2nd edition pp. 1704–1706. Ed.: Bergmeyer, H.N., New York-London: Academic Press 1974
Fowler, M.W.: Studies on the growth in culture of plant cells. XIV. Carbohydrate oxidation during the growth of Acer pseudoplatanus L. cells in suspension culture. J. Exp. Bot. 22, 715–724 (1971)
Fowler, M.W.: Carbohydrate metabolism and differentiation in seedling roots. New Phytol. 75, 461–478 (1975)
Fowler, M.W., Clifton, A.: Activities of enzymes of carbohydrate metabolism in cells of Acer pseudoplatanus L. maintained in continuous (chemostat) culture. Europ. J. Biochem. 45, 445–450 (1974)
Givan, C.V., Collin, H.A.: Studies on the growth in culture of plant cells. II. Changes in respiration rate and nitrogen content associated with the growth of Acer pseudoplatanus L. cells in suspension culture. J. exp. Bot. 18, 321–331 (1967)
Gamborg, O.L., Shyluk, J.P.: The culture of plant cells with ammonia as the sole nitrogen source. Plant Physiol. 45, 598–600 (1970)
Hahlbrock, K., Kuhlen, E.: Relationship between growth of parsley and soybean cells in suspension cultures and changes in the conductivity of the culture medium. Planta (Berl.) 108, 271–278 (1972)
Heimer, Y.M., Filner, P.: Regulation of the nitrate assimilation pathway in cultured tobacco cells. III. The nitrate uptake system. Biochim. biophys. Acta 230, 362–372 (1971)
Henshaw, G.G., Jha, K.K., Mehta, A.R., Shakeshaft, D.J., Street, H.E.: Studies on the growth in culture of plant cells. I. Growth patterns in batch propogated suspension cultures. J. Exp. Bot. 17, 362–377 (1966)
Jessup, W., Fowler, M.W.: Effect of the nitrogen source on the activities of key enzymes of carbohydrate metabolism in cultured plant cell in vitro. Biochem. Soc. Trans. 3, 1001–1003 (1975)
Jessup, W., Fowler, M.W.: Interrelationships between carbohydrate metabolism and nitrogen assimilation in plant cells grown in culture. I. Effect of glutamate and nitrate as alternative nitrogen sources on cell growth. Planta (Berl.) 132, 119–123 (1976)
King, J.: The uptake of nitrate by sycamore cells. 3rd International Congress of Plant Tissue and Cell Culture, Leicester. Abstract 133 (1974)
King, J., Hirji, R.: Amino acid transport systems of cultured soybean root cells. Canad. J. Bot. 53, 2088–2091 (1975)
King, P.J., Mansfield, K.J., Street, H.E.: Control of growth and cell division in plant cell suspension cultures. Canad. J. Bot. 51, 1807–1823 (1973)
Nash, D.T., Davies, M.E.: Some aspects of growth and metabolism of Paul's scarlet rose cell suspensions. J. Exp. Bot. 23, 75–91 (1972)
Sarkissian, G.S., Fowler, M.W.: Interrelationship between nitrate assimilation and carbohydrate metabolism in plant roots. Planta (Berl.) 119, 335–349 (1974)
Simpkins, I., Street, H.E.: Studies on the growth in culture of plant cells VII. Effects of kinetin on the carbohydrate and nitrogen metabolism of Acer pseudoplatanus L. cells grown in suspension culture. J. Exp. Bot. 21, 170–185 (1970)
Somogyi, M.: Notes on sugar determination. J. Biol. Chem. 195, 19–23 (1952)
Street, H.E., Gould, A.R., King, J.: Nitrogen assimilation and protein synthesis in plant cell cultures. In: Perspectives in experimental biology, 2, Botany pp. 337–356. Ed.: Sunderland, N. Oxford-New York: Pergammon 1976
Stuart, R., Street, H.E.: Studies on the growth in culture of plant cells. IV. The initiation of division in suspensions of stationary phase cells of Acer pseudoplatanus L. J. Exp. Bot. 20, 556–571 (1969)
Wilson, G.: Nutrition and differentiation of cells of Acer pseudoplatanus L. in suspension culture. Ph.D. thesis, University of Birmingham, England (1971)
Young, M.: Studies on the growth in culture of plant cells. XVI. Nitrogen assimilation during nitrogen limited growth of Acer pseudoplatanus L. cells in chemostat culture. J. Exp. Bot. 24, 1172–1185 (1973)
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Jessup, W., Fowler, M.W. Interrelationships between carbohydrate metabolism and nitrogen assimilation in cultured plant cells. Planta 132, 125–129 (1976). https://doi.org/10.1007/BF00388893
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DOI: https://doi.org/10.1007/BF00388893