Skip to main content

Subcellular localization of the red-absorbing form of phytochrome by immunocytochemistry

Planta volume 121, pages 119–131 (1974)Cite this article

Summary

An immunocytochemical technique was used to localize the red-absorbing form of phytochrome at the light- or electron-microscope level in etiolated barley (Hordeum vulgare L.) coleoptile tip, rice (Oryza sativa L.) coleoptilar node, maize (Zea mays L.) coleoptile tip, rye (Secale cereale L.) coleoptile tip and coleoptilar node, and oat (Avena sativa L.) root cap. Staining for phytochrome in the cells was found to be generally distributed throughout the cytoplasm. In addition, barley also showed staining around the periphery of vesicles, and rice showed staining in numerous discrete regions in the cytoplasm. Electron-microscopic localization studies of the nodal region of rye and the root cap of oat indicate staining associated with the nuclear membrane and with the interior of mitochondria and amyloplasts as well as general staining like that observed with the light microscope. Cells of the coleoptile tip of maize were unusual in having heavy staining associated with amyloplasts only.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Abbreviations

DAB:

3,3′-diaminobenzidine

PAP:

peroxidase-antiperoxidase complex

Pr:

red-absorbing form of phytochrome

Pfr:

far-red-absorbing form of phytochrome

References

  • Coleman, R. A.: Immunocytochemicl determination of phytochrome distribution and localization. Doct. dissert., Vanderbilt Univ., Nashville, Tenn., USA (1973)

    Google Scholar 

  • Coleman, R. A., Pratt, L. H.: Electron microscopic localization of phytochrome in plants using an indirect antibody labelling method. J. Histochem. Cytochem., in press (1974)

  • Fondeville, J. C., Borthwick, H. A., Hendricks, S. B.: Leaflet movement of Mimosa pudica L. indicative of phytochrome action. Planta (Berl.) 69, 357–364 (1966)

    Google Scholar 

  • Haupt, W.: Localization of phytochrome in the cell. Physiol. veg. 8, 551–563 (1970)

    Google Scholar 

  • Hökfelt, T., Fuxe, K., Goldstein, M., Joh, T.: Immunohistochemical localization of three catecholamine synthesizing enzymes: Aspects on methodology. Histochemie 33, 231–254 (1973)

    PubMed  Google Scholar 

  • Mackenzie, J. M., Jr., Coleman, R. A., Briggs, W. R., Pratt, L. H.: Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. Proc. nat. Acad. Sci. (Wash.), in press (1974)

  • Manabe, K., Furuya, M.: Phytochrome-dependent reduction of nicotinamide nucleotides in the mitochondrial fraction isolated from etiolated pea epicotyls. Plant Physiol. 53, 343–347 (1974)

    Google Scholar 

  • Marmé, D., Boisard, J., Briggs, W. R.: Binding properties in vitro of phytochrome to a membrane fraction. Proc. nat. Acad. Sci. (Wash.) 70, 3861–3865 (1973)

    Google Scholar 

  • Moriarty, G.: Adenohypophysis: Ultrastructural cytochemistry. A review. J. Histochem. Cytochem. 21, 855–894 (1973)

    PubMed  Google Scholar 

  • Newman, I. A., Briggs, W. R.: Phytochrome-mediated electric potential changes in oat seedlings. Plant Physiol. 50, 687–693 (1972)

    Google Scholar 

  • Pratt, L. H.: Comparative immunochemistry of phytochrome. Plant Physiol. 51, 203–209 (1973)

    Google Scholar 

  • Pratt, L. H., Coleman, R. A.: Immunocytochemical localization of phytochrome. Proc. nat. Acad. Sci. (Wash.) 68, 2431–2435 (1971)

    Google Scholar 

  • Pratt, L. H., Coleman, R. A.: Phytochrome distribution in etiolated grass seedlings as assayed by an indirect antibody-labelling method. Amer. J. Bot. 61, 195–202 (1974)

    Google Scholar 

  • Pratt, L. H., Kidd, G. H., Coleman, R. A.: An immunochemical characterization of the phytochrome destruction reaction. Biochim. biophys. Acta (Amst.) 365, 93–107 (1974)

    Google Scholar 

  • Quail, P. H., Schäfer, E.: Particle-bound phytochrome: a function of light dose and steadystate level of the far-red-absorbing form. J. Membrane Biol. 15, 393–404 (1974)

    Google Scholar 

  • Racusen, R., Miller, K.: Phytochrome-induced adhesion of mung bean root tips to platinum electrodes in a direct current field. Plant Physiol. 49, 654–655 (1972)

    Google Scholar 

  • Rubinstein, B., Drury, K. S., Park, R. B.: Evidence for bound phytochrome in oat seedlings. Plant Physiol. 44, 105–109 (1969)

    Google Scholar 

  • Satter, R. L., Marinoff, P., Galston, A. W.: Phytochrome controlled nyctinasty in Albizzia julibrissin. II. Potassium flux as a basis for leaflet movement. Amer. J. Bot. 57, 916–926 (1970)

    Google Scholar 

  • Sternberger, L. A., Hardy, P. H., Jr., Cuculis, J. J., Meyer, H. G.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigenantibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)

    PubMed  Google Scholar 

  • Tanada, T.: A rapid photoreversible response of barley root tips in the presence of 3-indoleacetic acid. Proc. nat. Acad. Sci. (Wash.) 59, 376–380 (1968)

    Google Scholar 

  • Wellburn, F. A. M., Wellburn, A. R.: Response of etioplasts in situ and in isolated suspensions to pre-illumination with various combinations of red, far-red and blue light. New Phytol. 72, 55–60 (1973)

    Google Scholar 

Download references

Author information

Author notes

  1. Richard A. Coleman

    Present address: Laboratory of Dental Research, Dental School, University of North Carolina, 27514, Chapel Hill, North Carolina, USA

Affiliations

  1. Department of Biology, Vanderbilt University, 37235, Nashville, Tennessee, USA

    Richard A. Coleman & Lee H. Pratt

Authors
  1. Richard A. Coleman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lee H. Pratt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Coleman, R.A., Pratt, L.H. Subcellular localization of the red-absorbing form of phytochrome by immunocytochemistry. Planta 121, 119–131 (1974). https://doi.org/10.1007/BF00388751

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388751

Keywords

  • Maize
  • Light Microscope
  • Subcellular Localization
  • Nodal Region
  • Nuclear Membrane