Skip to main content
SpringerLink
Log in

Control of mitochondrial activities by phytochrome during greening

  • Published:
Planta Aims and scope Submit manuscript

Abstract

Mitochondria isolated from 7-day old darkgrown Avena sativa L. (var. Arnold) laminae given 5 min illumination of red light, followed by varying lengths of darkness up to 3 h, showed at least a twofold increase in the rates of both NADH-dependent oxygen consumption and respiratory chain phosphorylation over those of mitochondria isolated from unilluminated tissue. Similar organelles, isolated from tissue given either far-red or red followed by far-red pretreatment, exhibited rates of both functions of between 25% and 75% below those of the mitochondria from unilluminated tissue. The induction-reversion criteria for phytochrome control of respiration and oxidative phosphorylation were satisfied under all experimental conditions during the greening process.

Treatment with continuous far-red light, acting presumably through the ‘high irradiance’ reaction of phytochrome, served to disengage phytochrome activity from photosynthesis. The stimulation of oxidative phosphorylation still occurred under these conditions, slightly slower but much more prolonged in the absence of ATP from photophosphorylation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BSA:

bovine serum albumen

DAD:

diaminodurene

EDTA:

ethylene-diaminetetra-acetic acid

HEPES:

N-2-hydroxy-ethyl-piperazine-N′-2-ethane-sulphonic acid

Pfr :

phytochrome in the active form

References

  • Bajracharya, D., Falk, H., Schopfer, P.: Phytochrome-mediated development of mitochondria in the cotyledons of mustard (Sinapis alba L.) seedlings. Planta 131, 253–261 (1976)

    Google Scholar 

  • Bürcky, K., Kauss, H.: Change in ATP and ADP levels of mung bean root tips after irradiation with red light. Z. Pflanzenphysiol 73, 184–186 (1974)

    Google Scholar 

  • Chow, W., Hope, A.B.: Proton translocation, electron transport and photophosphorylation in isolated chloroplasts. Aust. J. Plant Physiol. 4, 647–665 (1977)

    Google Scholar 

  • Davis, E.J., Davis-van Thienen, W.I.A.: Control of mitochondrial metabolism by the ATP/ADP ratio. Biochem. Biophys. Res. Commun. 83, 1260–1266 (1978)

    PubMed  Google Scholar 

  • DeGreef, J.A., Caubergs, R., Verbelen, J.P., Moereels, E.: Phytochrome-mediated inter-organ dependence and rapid transmission of the light stimulus. In: Light and Plant Development, pp. 295–336, Smith, H., ed. London: Butterworth 1976

    Google Scholar 

  • Delieu, T., Walker, D.A.: An improved cathode for the measurement of photosynthetic oxygen evolution by isolated chloroplasts. New Phytol. 71, 201–225 (1972)

    Google Scholar 

  • Feierabend, J., Beevers, H.: Developmental studies on microbodies in wheat leaves I. Conditions influencing enzyme development. Plant Physiol. 49, 28–32 (1972)

    Google Scholar 

  • Friedrich, K.E., Mohr, H.: Adenosine 5′-triphosphate content and energy charge during photomorphogenesis of the mustard seedling Sinapis alba L. Photochem. Photobiol. 22, 49–53 (1975)

    PubMed  Google Scholar 

  • Hampp, R.: Kinetics of mitochondrial phosphate transport and rates of respiration and phosphorylation during greening of etiolated Avena leaves. Planta 144, 325–332 (1979)

    Google Scholar 

  • Hampp, R., Wellburn, A.R.: Changes in the permeability of the inner mitochondrial membrane associated with plastid development. Planta 131, 21–26 (1976)

    Google Scholar 

  • Hampp, R., Wellburn, A.R.: Translocation and phosphorylation of adenine nucleotides by mitochondria and plastids during greening. Z. Pflanzenphysiol. (in press)

  • Hartmann, K.M.: Wirkungspektrum der Photomorphogenese unter Hochenergiebedingungen und seine Interpretation auf der Basis des Phytochroms (Hypokotylwachstumshemmung bei Lactuca sativa L.). Z. Naturforsch. 226, 1172–1175 (1967)

    Google Scholar 

  • Hock, B., Kühnert, E., Mohr, H.: Die Regulation von Fettabbau und Atmung bei Senfkeimlingen durch Licht (Sinapis alba L.). Planta 65, 129–138 (1965)

    Google Scholar 

  • Hock, B., Mohr, H.: Die Regulation der O2-Aufnahmen von Senfkeimlingen (Sinapis alba L.) durch Licht. Planta 61, 209–228 (1964)

    Google Scholar 

  • José, A.M.: Phytochrome modulation of ATPase activity in a membrane fraction from Phaseolus. Planta 137, 203–206 (1977)

    Google Scholar 

  • Kagawa, T., McGregor, D.I., Beevers, H.: Development of enzymes in the cotyledons of water melon seedlings. Plant Physiol. 51, 66–71 (1973)

    Google Scholar 

  • Klingenberg, M.: Mitochondria metabolite transport. FEBS Lett. 6, 145–154 (1970)

    Article  PubMed  Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurements with the Folin phenol regent. J. Biol. Chem. 193, 265–275 (1951)

    PubMed  Google Scholar 

  • Masoner, M., Unser, C., Mohr, H.: Accumulation of protochlorophyll and chlorophyll a as controlled by photomorphogenetically effective light. Planta 105, 267–272 (1972)

    Google Scholar 

  • McGivan, J.D., Grebe, K., Klingenberg, M.: On the coupling between the transport of phosphate and adenine nucleotides in rat liver mitochondria. Biochem. Biophys. Res. Commun. 45, 1533–1541 (1971)

    PubMed  Google Scholar 

  • Mitchell, P., Moyle, J.: Translocation of some anions, Cations and acids in rat liver mitochondria. Eur. J. Biochem. 9, 149–155 (1969)

    PubMed  Google Scholar 

  • Mohr, H.: Lectures on photomorphogenesis. Berlin, Heidelberg, New York: Springer 1972

    Google Scholar 

  • Oelze-Karow, H., Mohr, H.: Control of chlorophyll b biosynthesis by phytochrome. Photochem. Photobiol. 27, 189–193 (1978)

    Google Scholar 

  • Ogawa, T., Shibata, K.: A sensitive method for determining chlorophyll b in plant extracts. Photochem. Photobiol. 4, 193–200 (1965)

    Google Scholar 

  • Öpik, H.: Mitochondria. In: Dynamic Aspects of Plant Ultrastructure, pp. 52–83, Robards, A.W., ed. Maidenhead: McGraw-Hill 1974

    Google Scholar 

  • Pfaff, E., Klingenberg, M.: Adenine nucleotide translocation of mitochondria. I. Specificity and control. Eur. J. Biochem. 6, 66–79 (1968)

    PubMed  Google Scholar 

  • Schäfer, E.: A new approach to explain the “high irradiance responses” of photomorphogenesis on the basis of phytochrome. J. Math. Biol. 2, 41–56 (1975)

    Article  Google Scholar 

  • Schmidt, H.W., Hampp, R.: Regulation of membrane properties of mitochondria and plastids during chloroplast development. II. The action of phytochrome in a cell-free system. Z. Pflanzenphysiol. 82, 428–434 (1977)

    Google Scholar 

  • Schopfer, P.: Experimente zur Pflanzenphysiologie, pp. 404–406. Freiburg: Rombach 1970

    Google Scholar 

  • Schopfer, P., Bajracharya, D., Falk, H., Thien, W.: Phytochromgesteuerte Entwicklung von Zellorganellen (Plastiden, Microbodies, Mitochondrien). Ber. Dtsch. Bot. Ges. 88, 245–268 (1975)

    Google Scholar 

  • Slater, E.C., Rosing, J., Mol, A.: The phosphorylation potential generated by respiring mitochondria. Biochim. Biophys. Acta 292, 534–553 (1973)

    PubMed  Google Scholar 

  • Weischet, W.: Thesis (Diploma), University of Freiburg (F.R.G.) cited in Friederich, K.E., Mohr, H.: Adenosine 5′-triphosphate content and energy charge during photomorphogenesis of the mustard seedling Sinapis alba L. Photochem. Photobiol. 22, 49–53 (1975)

    PubMed  Google Scholar 

  • Wellburn, A.R., Hampp, R.: Appearance of photochemical function in prothylakoids during plastid development. Biochim. Biophys. Acta 547, 380–397 (1979)

    PubMed  Google Scholar 

  • White, J.M., Pike, C.S.: Rapid phytochrome-mediated changes in adenosine 5′-triphosphate content of etiolated bean buds. Plant Physiol 53, 76–79 (1974)

    Google Scholar 

  • Yunghans, H., Jaffe, M.J.: Rapid respiratory changes due to red light or acetylcholine during the early events of phytochromemediated photomorphogenesis. Plant Physiol. 49, 1–7 (1972)

    Google Scholar 

Download references

Author information

Author notes

  1. A. R. Wellburn

    Present address: Department of Biological Sciences, University of Lancaster, LA1 4YQ, Lancaster, U.K.

Authors and Affiliations

  1. Lenrstuhl für Botanik, Technische Universität München, Arcisstraße 21, D-8000, München 2, Federal Republic of Germany

    R. Hampp & A. R. Wellburn

Authors
  1. R. Hampp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. R. Wellburn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hampp, R., Wellburn, A.R. Control of mitochondrial activities by phytochrome during greening. Planta 147, 229–235 (1979). https://doi.org/10.1007/BF00388743

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388743

Key words

Search

Navigation