Abstract
The distribution of lectin activity in soybean and peanut plants has been investigated. In both plants activity is found in all tissues examined (roots, shoots and leaves) at all stages of development from seedling to maturity (7 weeks). The cellular location of the lectins differs between soybean and peanut: in soybean the lectins are generally membrane-associated, whereas in peanut plants lectin activity is present also in the soluble cytoplasmic fraction. The membrane-associated lectins appear to differ from the seed lectins of the respective plants. The function of membrane-associated lectins is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- RCA:
-
lectin of castor bean
- SBA:
-
soybean agglutinin
- PNA:
-
peanut agglutinin
- HEPES:
-
2-[4-(2-Hydroxyethyl)-piperazinyl-(1)]ethanesulphonic acid
- MES:
-
morpholinoethane sulphonic acid
- PBS:
-
phosphate-buffered saline
References
Axen, R., Porath, J., Ernback, S.: Chemical coupling of peptides and proteins to polysaccharides by means of cyranogen halides. Nature 214, 1302–1304 (1967)
Bowles, D.J., Kauss, H.: Carbohydrate-binding proteins from cellular membranes of plant tissues. Plant Sci. Lett. 4, 411–418 (1975)
Bowles, D.J., Kauss, H.: Characterization, enzymatic and lectin properties of isolated membranes from Phaseolus aureus. Biochim. Biophys. Acta 443, 360–374 (1976)
Bowles, D.J., Schnarrenberger, C., Kauss, H.: Lectins as membrane components of mitochondria from Ricinus communis. Biochem. J. 160, 375–382 (1976)
Ersson, B., Asperg, K., Porath, J.: The phytohemagglutinin from sunn hemp seeds. Purification by biospecific affinity chromatography. Biochim. Biophys. Acta 310, 446–452 (1973)
Gold, E.: Clumping and sedimentation pattern manifestations of haem-agglutination. Vox Sang. 15, 222–231 (1968)
Irimura, T., Kawaguchi, T., Terao, T., Osawa, t.: Carbohydrate-binding specifity of the so-called galactose-specific phytohem-agglutinins. Carbohydrate Res. 39, 317–327 (1975)
Lis, H., Sharon, N.: The biochemistry of plant lectins (phytohem-agglutinins). Ann. Rev. Biochem. 42, 541–574 (1973)
Lis, H., Sharon, N.: Lectins, their chemistry and application to immunology. The Antigens, Vol. IV, pp. 429–529, Sela, M., New York: Academic Press 1977
Lotan, R., Skutelsky, E., Danon, D., Sharon, N.: The Purification, composition and specificity of the Anti-T lectin from Peanut (Arachis hypogaea). J. Biol. Chem. 250, 8518–8523 (1975)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Miron, T., Carter, W.G., Wilchek, M.: Polyacrylhydrazido-agarose as a support for immobilization of enzymes, lectins, antibodies and small ligands. J. Solid-Phase Biochem. 1, 225–236 (1975)
Pont-Lezica, R., Romero, P.A., Hopp, H.E.: Glucosylation of membrane-bound proteins by lipid-linked glucose. Planta 140, 177–183 (1978)
Pueppke, S.G., Bauer, W.D., Keegstra, K., Ferguson, a.L.: The role of lectins in plant-microorganism interactions II: Distribution of soybean lectin in tissues of Glycine max. Plant Physiol. 61, 779–784 (1978)
Sharon, N., Lis, H.: Lectins: cell agglutinating and sugar-specific proteins. Science 177, 949–959 (1972)
Tully, R.E., Beevers, H.: Protein bodies of castor bean endosperm. Plant Physiol. 58, 710–716 (1976)
Youle, R.J., Huang, A.H.C.: Protein bodies from the endosperm of castor bean. Plant Physiol. 58, 703–708 (1976)