Summary
The formation of zoospores or gametes in the green alga Ulva mutabilis Føyn is shown to be blocked by some substance(s) which can be extracted into fresh growth medium from a suspension of living thallus fragments. The inhibiting power of thallus extract is greatly reduced by heat treatment, but heat-stable components may also be present since active extracts can be produced by boiling algal thalli in growth medium. Separation of the extract on a Sephadex G25 column gave two peaks of inhibitory activity which corresponded with the fraction's content of high molecular carbohydrate (probably ulvin). Various mono- and disaccharides were inactive. In order to block meiotic zoospore formation completely, thallus extracts must be applied at least 6–7 h prior to the cells' visible entry into the meiotic prophase.
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References
Bühnemann, F.: Untersuchungen über einen Sporulations- und Wachstumshemmstoff in Oedogonium-Kulturen. Arch. Mikrobiol. 23, 14–27 (1955)
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebess, P. A., Smith, F.: Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28, 350–356 (1956)
Föyn, B.: Geschlechtskontrollierte Vererbung bei der marinen Grünalge Ulva mutabilis. Arch. Protistenk. 104, 236–253 (1959)
Hustede, H.: Untersuchungen über die stoffliche Beeinflussung der Entwicklung von Stigeoclonium falklandicum und Vaucheria sessilis durch Tryptophanabkömmlinge. Biol. Zbl. 76, 555–595 (1957)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randal, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Nordby, Ø.: Light microscopy of meiotic zoosporogenesis and mitotic gametogenesis in Ulva mutabilis Föyn. J. Cell. Sci. 15 443–455 (1974)
Nordby, Ø., Hoxmark, R. Ch.: Changes in cellular parameters during synchronous meiosis in Ulva mutabilis Føyn. Exp. Cell. Res. 75, 321–328 (1972)
Page, J. Z., Kingsbury, J. M.: Culture studies on the marine green alga Halicystis parvula — Derbesia tenuissima. II. Synchrony and periodicity in gamete formation and release. Amer. J. Bot. 55, 4–11 (1968)
Percival, E., Wold, J. K.: The acid polysaccharide from the green seaweed Ulva lactuca. Part II. The site of the ester sulphate. J. chem. Soc. 1040 5456–5468 (1963)
Scott, T. A., Melvin, E. H.: Determination of dextran with anthron. Analyt. Chem. 25, 1956 (1953)
Thiadens, A. J. H., Zeuthen, E.: Meiosis and sporulation induced in sporophytes of Ulva mutabilis (“Slender”) with synchronous mitosis. Planta (Berl.) 72, 60–65 (1967)
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Nilsen, G., Nordby, Ø. A sporulation-inhibiting substance from vegetative thalli of the green alga Ulva mutabilis, Føyn. Planta 125, 127–139 (1975). https://doi.org/10.1007/BF00388699
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DOI: https://doi.org/10.1007/BF00388699