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Cell wall assembly in vitro from Chlamydomonas reinhardi

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Summary

The conditions for in vitro dissociation and reassembly of the cell wall of Chlamydomonas reinhardi have been investigated. The cell wall dissociates in aqueous 8 M lithium chloride to two homogeneous components (6.8 S20w and 9.3 S20w)and dialysis of the cell wall subunits against water causes reassembly of a product having the same overall morphology and chemically similar to the original cell walls. Cell wall subunits in 8 M lithium chloride alone do not reassemble on dialysis against water to form cell walls but a nucleation centre has to be provided.

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NOR 70F, Norwich, U.K.

    G. J. Hills

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  1. G. J. Hills
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Hills, G.J. Cell wall assembly in vitro from Chlamydomonas reinhardi . Planta 115, 17–23 (1973). https://doi.org/10.1007/BF00388601

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  • DOI: https://doi.org/10.1007/BF00388601

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