Skip to main content
SpringerLink
Log in

Katabolism of plant cytoplasmic ribosomes: A study of the interaction between ribosomes and ribonuclease

  • Published:
Planta Aims and scope Submit manuscript

Summary

Ribosome preparations from several species of flowering plant were found to contain ribonuclease. It was predominantly latent in ionic conditions which maintained the integrity of the ribosomes but was activated by agents which disrupted ribosome structure by chelating their divalent cations. The ribonuclease was not a structural component of the ribosomes but was preferentially adsorbed by them upon disruption of cells. Adsorption took place onto both large and small ribosomal subunits, and onto monosomes and polysomes. These results are discussed in relation to possible mechanisms for the breakdown of ribosomal RNA in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Acton, G. J.: Phytochrome control of the level of extractable ribonuclease activity in etiolated hypocotyls. Nature (Lond.) New Biol. 236, 255–256 (1972)

    Google Scholar 

  • Acton, G. J., Lewington, R. J., Myers, A.: The effect of light upon ribonuclease activity of Lupinus albus hypocotyls. Biochim. biophs. Acta (Amst.) 204, 144–155 (1970)

    Google Scholar 

  • Beevers, H., Stiller, M. L., Butt, V. S.: Metabolism of the organic acids. In: Plant physiology, a treatise, vol. IVB, p. 119–262 (F. C. Steward, ed.). New York and London: Acad. Press 1966

    Google Scholar 

  • Breen, M. D., Whitehead, E. I., Kenefick, D. G.: Requirements for the extraction of polyribosomes from barley tissue. Plant Physiol. 49, 733–739 (1971)

    Google Scholar 

  • Cammarano, P., Romeo, A., Gentile, M., Felsani, A., Gaulerzi, C.: Size heterogeneity of the large ribosomal subunits and conservation of the small subunits in eucaryote evolution. Biochim. biophys. Acta (Amst.) 281, 597–624 (1972)

    Google Scholar 

  • Davies, D. D.: Control of and by pH. In: Rate control of biological processes. Symp. Soc. exp. Biol. 27, 513–529 (1973)

    PubMed  Google Scholar 

  • Drawert, H.: Vitalfärbung und Vitalfluorochromierung pflanzlicher Gewebe. Protoplasmatologia 11, D3, 516–517 (1968)

    Google Scholar 

  • Dyer, T. A., Osborne, D. J.: Leaf nucleic acids. II. Metabolism during senescence and the effect of kinetin. J. exp. Bot. 22, 552–560 (1971)

    Google Scholar 

  • Egami, F., Takahashi, K., Uchida, T.: Ribonucleases in Taka-Diastase: properties, chemical nature, and applications. In: Progress in nucleic acid research and molecular biology, p. 59–101 (Davidson, J. N., Cohn, W. E., eds.) New York and London: Acad. Press 1964

    Google Scholar 

  • Gagnon, C., de Lamirande, G.: Autodegradation of RNA of plant cell ribosomes. Arch. Biochem. Biophys. 150, 573–577 (1972)

    PubMed  Google Scholar 

  • Godson, G. N., Cox, R. A.: Structure of Escherichia coli ribosomes: effect of ribonuclease on the 30S and 50S subunits. Biochim. biophys. Acta (Amst.) 204, 489–501 (1970)

    Google Scholar 

  • Hamilton, M. G., Ruth, M. E.: The dissociation of rat liver ribosomes by ethylenediaminetetraacetic acid; molecular weights, chemical composition, and buoyant densities of the subunits. Biochemistry 8, 851–856 (1969)

    PubMed  Google Scholar 

  • Hsiao, T. C.: Ribonuclease associated with ribosomes of Zea mays. Plant Physiol. 43, 1355–1361 (1968)

    Google Scholar 

  • Johnston, F. B., Stern, H.: Mass isolation of viable wheat embryos. Nature (Lond.) 179, 160–161 (1957)

    Google Scholar 

  • Loening, U. E.: The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 102, 251–259 (1967)

    PubMed  Google Scholar 

  • Loening, U. E.: The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 113, 131–138 (1969)

    PubMed  Google Scholar 

  • Matile, Ph.: Aleurone vacuoles as lysosomes. Z. Pflanzenphysiol. 58, 365–368 (1968)

    Google Scholar 

  • Matile, Ph., Winkenbach, F.: Function of lysosomes and lysosomal enzymes in the senescing corolla of the morning glory (Ipomoea purpurea). J. exp. Bot. 22, 759–771 (1971)

    Google Scholar 

  • Parish, J. H., Kirby, K. S.: Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim. biophys. Acta (Amst.) 129, 554–562 (1966)

    Google Scholar 

  • Payne, E. S., Boulter, D., Brownrigg, A., Lonsdale, D., Yarwood, A., Yarwood, J. N.: A polyuridylic acid-directed cell-free system from 60 day-old developing seeds of Vicia faba. Phytochemistry 10, 2293–2298 (1971)

    Article  Google Scholar 

  • Payne, P. I., Boulter, D.: Katabolism of plant cytoplasmic ribosomes. RNA breakdown in the senescent cotyledons of germinating broad bean seedlings. Planta (Berl.) 117, 251–258 (1974)

    Google Scholar 

  • Payne, P. I., Loening, U. E.: Ribosome breakdown accompanying the isolation of pea root microsomes. An analysis by polyacrylamide gel electrophoresis. Biochim. biophys. Acta (Amst.) 224, 128–135 (1970)

    Google Scholar 

  • Penon, P., Cecchini, J., Miassod, R., Ricard, J., Teissere, M., Pinna, M.: Peroxidases associated with lentil root ribosomes. Phytochem. 9, 73–86 (1970)

    Article  Google Scholar 

  • Pitt, D., Galpin, M.: Isolation and properties of lysosomes from dark-grown potato shoots. Planta (Berl.) 109, 233–258 (1973)

    Google Scholar 

  • Roberts, B. E., Paterson, B. M.: Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc. nat. Acad. Sci. (Wash.) 70, 2330–2334 (1973)

    Google Scholar 

  • Roberts, B. E., Payne, P. I., Osborne, D. J.: Protein synthesis and the viability of rye grains. Loss of activity of protein-synthesizing systems in vitro associated with a loss of viability. Biochem. J. 131, 275–286 (1973)

    PubMed  Google Scholar 

  • Small, J.: Modern aspects of pH with special reference to plants and soils. London: Baillìere, Tindall and Cox 1954

    Google Scholar 

  • Trewavas, A.: The turnover of nucleic acids in Lemna minor. Plant Physiol. 45, 742–751 (1970)

    Google Scholar 

  • Villiers, T. A.: Lysosomal activities of the vacuole in damaged and recovering plant cells. Nature (Lond.) New Biol. 233 57–58 (1971)

    Google Scholar 

  • Wilson, C. M.: Plant nucleases. I. Separation and purification of two ribonucleases and one nuclease from corn. Plant Physiol. 43, 1332–1338 (1968)

    Google Scholar 

  • Wilson, C. M.: Plant nucleases. III. Polyacrylamide gel electrophoresis of corn ribonuclease isoenzymes. Plant Physiol. 48, 64–68 (1971)

    Google Scholar 

  • Wyen, N. V., Farkas, G. L.: On the ribonuclease associated with cytoplasmic ribosomes isolated from Avena leaves. Biochem. Physiol. Pflanzen 162, 220–224 (1971)

    Google Scholar 

  • Yarwood, J. N.: Ph. D. Thesis, University of Durham (1973)

Download references

Author information

Authors and Affiliations

  1. Agricultural Research Council Unit of Developmental Botany, 181A Huntingdon Road, CB3 ODY, Cambridge, UK

    T. A. Dyer & P. I. Payne

Authors
  1. T. A. Dyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. I. Payne
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dyer, T.A., Payne, P.I. Katabolism of plant cytoplasmic ribosomes: A study of the interaction between ribosomes and ribonuclease. Planta 117, 259–268 (1974). https://doi.org/10.1007/BF00388399

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388399

Keywords

Search

Navigation