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Investigation of some enzymatic activities contributing to the biosynthesis of galactolipid precursors in Vicia faba

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Summary

Assay conditions, intracellular distribution and change of activity during greening of etiolated seedlings of Vicia faba L. have been investigated for aldose reductase (EC 1.1.1.21), UDP-glucose pyrophosphorylase (EC 2.7.7.9), UDP-glucose 4-epimerase (EC 5.1.3.2) and phosphatidate phosphatase (EC 3.1.3.4). The first three enzymes were found to be soluble proteins of the cytoplasm, whereas phosphatidate phosphatase was found in the soluble and a sedimentable (15000 Xg) fraction.

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References

  • Barron, E. J., Stumpf, P. K.: Biosynthesis of triglycerides by avocado mesocarp enzymes. Biochim. biophys. Acta (Amst.) 60, 329–337 (1962)

    Article  Google Scholar 

  • Bergmeyer, H. U.: Methoden der enzymatischen Analyse, 2. Aufl., Bd. I. Weinheim: Verlag Chemie 1970

    Google Scholar 

  • Bird, I. F., Cornelius, M. J., Dyer, T. A., Keys, A. J.: The purity of chloroplasts isolated in non-aqueous media. J. exp. Bot. 24, 211–215 (1973)

    Google Scholar 

  • Bird, I. F., Porter, H. K., Stocking, C. R.: Intracellular localization of enzymes associated with sucrose synthesis in leaves. Biochim. biophys. Acta (Amst.) 100, 366–375 (1965)

    Google Scholar 

  • Blank, M. L., Snyder, F.: Specificities of alkaline and acid phosphatases in the dephosphorylation of phospholipids. Biochemistry (Wash.) 9, 5034–5036 (1970)

    Google Scholar 

  • Brooks, J. L., Stumpf, P. K.: Fat metabolism in higher plants. XXIX. Properties of a soluble fatty acid synthesizing system from lettuce chloroplasts. Arch. Biochem. Biophys. 116, 108–116 (1966)

    PubMed  Google Scholar 

  • Bruinsma, J.: A comment on the spectrophotometric determination of chlorophyll. Biochim. biophys. Acta (Amst.) 52, 576–578 (1961)

    Article  Google Scholar 

  • Chang, S. B., Kulkarni, N. D.: Enzymatic reactions for galactolipid synthesis with a soluble, sub-chloroplast fraction from Spinacia oleracea. Phytochemistry 9, 927–934 (1970)

    Article  Google Scholar 

  • Cheniae, G. M.: Phosphatidic acid and glyceride synthesis by particles from spinach leaves. Plant Physiol. 40, 235–243 (1965)

    PubMed  Google Scholar 

  • Douce, R.: Incorporation de l'acide phosphatidique dans le CDP-diglycéride des mitochondries isolées des inflorescences de chou-fleur. C. R. Acad. Sci. (Paris) 272, 3146–3149 (1971)

    Google Scholar 

  • Douce, R., Guillot-Salomon, T.: Sur l'incorporation de la radioactivité du snglycérol-3-phosphate-14C dans le monogalactosyldiglycéride des plastes isolés. FEBS Letters 11, 121–124 (1970)

    Article  PubMed  Google Scholar 

  • Eccleshall, T. R., Hawke, J. C.: Biosynthesis of monogalactosyl diglyceride by chloroplasts from Spinacia oleracea and from some gramineae. Phytochemistry 10, 3035–3045 (1971)

    Article  Google Scholar 

  • Frank, G.: Physiologie und Biochemie der Glycerinbiosynthese in Dunaliella tertiolecta. Dissert., Fachb. Chemie, Univ. Tübingen (1972)

  • Ginsburg, V.: Purification of UDP-glucose pyrophosphorylase from mung bean seedlings. J. biol. Chem. 232, 55–61 (1958)

    PubMed  Google Scholar 

  • Göring, H., Reckin, E.: Einfluß der D-Galaktose auf den Kohlenhydratstoffwechsel pflanzlicher Gewebe. Flora (Abt. A) 159, 82–103 (1968)

    Google Scholar 

  • Gustafson, G. L., Gander, J. E.: UDP-glucose pyrophosphorylase from Sorghum vulgare. Purification and kinetic properties. J. biol. Chem. 247, 1387–1397 (1972)

    PubMed  Google Scholar 

  • Hajra, A. K., Agranoff, B. W.: Phosphatidate phosphatase from pig brain. In: Methods in enzymology, Lowenstein, J. M., ed., vol. 14, p. 185–188. New York, London: Acad. Press 1969

    Google Scholar 

  • Hansen, R. G., Albrecht, G. J., Bass, S. T., Seifert, L. L.: UDP-glucose pyrophosphorylase (crystalline) from liver. In: Methods in enzymology, Neufeld, E. F., Ginsburg, V., eds., vol. 8, p. 248–253. New York, London: Acad. Press 1966

    Google Scholar 

  • Hassid, W. Z., Putman, E. W., Ginsburg, V.: Metabolism of galactose in Canna leaves and wheat seedlings. Biochim. biophys. Acta (Amst.) 20, 17–22 (1956)

    Article  Google Scholar 

  • Heber, U., Willenbrink, J.: Sites of synthesis and transport of photosynthetic products within leaf cells. Biochim biophys. Acta (Amst.) 82, 313–324 (1964)

    Google Scholar 

  • Heinrich, M. R., Howard, S. M.: Galactokinase. In: Methods in enzymology, Wood, W. A., ed., vol. 9, p. 407–412. New York, London: Acad. Press 1966

    Google Scholar 

  • Heinz, E.: Some properties of the acyl galactosyl diglyceride-forming enzyme from leaves. Z. Pflanzenphysiol. 69, 359–376 (1973)

    Google Scholar 

  • Hers, H. G.: L'aldose-réductase. Biochim. biophys. Acta (Amst.) 37, 120–126 (1960)

    Article  Google Scholar 

  • Hoffman, F., Kull, U., Jeremias, K.: Markierung von Kohlenhydraten in Blättern nach Fütterung von 14C-Galaktose. Z. Pflanzenphysiol. 64, 223–231 (1971)

    Google Scholar 

  • Huber, W., Fekete, M. A. R. de, Ziegler, H.: Enzyme des Stärkeumsatzes in Bündelscheiden- und Palisadenchloroplasten von Zea mays. Planta (Berl.) 87, 360–364 (1969)

    Google Scholar 

  • Hue, L., Hers, H. G.: The conversion of [4-3H]fructose and of [4-3H]glucose to liver glycogen in the mouse. An investigation of the glyceraldehyde crossroads. Europ. J. Biochem. 29, 268–275 (1972)

    PubMed  Google Scholar 

  • Kates, M.: Hydrolysis of lecithin by plant plastid enzymes. Canad. J. Biochem. 33, 575–589 (1955)

    Google Scholar 

  • Kates, M., Sastry, P. S.: Phospholipase D. In: Methods in enzymology Lowenstein, J. M., ed., vol. 14, p. 197–204. New York, London: Acad. Press 1969

    Google Scholar 

  • Lowry, O. H., Lopez, J. A.: The determination of inorganic phosphate in the presence of labile phosphate esters. J. biol. Chem. 162, 421–428 (1946)

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    PubMed  Google Scholar 

  • Marshall, M. O., Kates, M.: Biosynthesis of phosphatidylglycerol by cell-free preparations from spinach leaves. Biochim. biophys. Acta (Amst.) 260, 558–570 (1972)

    Google Scholar 

  • Mazliak, P., Oursel, A., Ben Abdelkader, A., Grosbois, M.: Biosynthèse des acides gras dans les mitochondries végétales isolées. Europ. J. Biochem. 28, 399–411 (1972)

    PubMed  Google Scholar 

  • Moore, T. S., Lord, J. M., Kagawa, T., Beevers, H.: Enzymes of phospholipid metabolism in the endoplasmic reticulum of castor bean endosperm. Plant Physiol. 52, 50–53 (1973)

    Google Scholar 

  • Mudd, J. B., van Vliet, H. H. D. M., van Deenen, L. L. M.: Biosynthesis of galactolipids by enzyme preparations from spinach leaves. J. Lipid Res. 10, 623–630 (1969)

    PubMed  Google Scholar 

  • Neufeld, E. F., Ginsburg, V., Putman, E. W., Fanshier, D., Hassid, W. Z.: Formation and interconversion of sugar nucleotides by plant extracts. Arch. Biochem. Biophys. 69, 602–616 (1957)

    PubMed  Google Scholar 

  • Neufeld, E. F., Hall, C. W.: Formation of galactolipids by chloroplasts. Biochem. biophys. Res. Commun. 14, 503–508 (1964)

    PubMed  Google Scholar 

  • Ongun, A., Mudd, J. B.: Biosynthesis of galactolipids in plants. J. biol. Chem. 243, 1558–1566 (1968)

    PubMed  Google Scholar 

  • Ongun, A., Mudd, J. B.: The biosynthesis of steryl glucosides in plants. Plant Physiol. 45, 255–262 (1970)

    PubMed  Google Scholar 

  • Randall, D. D., Tolbert, N. E.: 3-Phosphoglycerate phosphatase in plants. I. Isolation and characterization from sugarcane leaves. J. biol. Chem. 246, 5510–5517 (1971)

    PubMed  Google Scholar 

  • Rubery, P. H.: The activity of UDP-glucose 4-epimerase in cambial tissue and differentiating xylem isolated from sycamore (Acer pseudoplatanus) trees. Planta (Berl.) 111, 267–269 (1973)

    Google Scholar 

  • Santarius, K. A., Stocking, C. R.: Intracellular localization of enzymes in leaves and chloroplast membrane permeability to compounds involved in amino acid syntheses. Z. Naturforsch. 24b, 1170–1179 (1969)

    Google Scholar 

  • Sastry, P. S., Kates, M.: Biosynthesis of lipids in plants. II. Incorporation of glycerophosphate-32P into phosphatides by cell-free preparations from spinach leaves. Canad. J. Biochem. 44, 459–467 (1966)

    Google Scholar 

  • Sherratt, D., Givan, C. V.: The apparent absence of a pathway for synthesis of acetyl coenzyme A in pea chloroplasts: lack of 14CO2 incorporation into lipids by the isolated organelle. Planta (Berl.) 113, 47–52 (1973)

    Google Scholar 

  • Smillie, R. M.: Formation and function of soluble proteins in chloroplasts. Canad. J. Bot. 41, 123–154 (1963)

    Google Scholar 

  • Stumpf, P. K.: Fatty acid metabolism in plant tissues. In: Comprehensive biochemistry, Florkin, M., Stotz, E. H., eds., vol. 18, p. 265–292. Amsterdam: Elsevier 1970

    Google Scholar 

  • Tovey, K. C., Roberts, R. M.: Studies on the pyrophosphorylases of wheat. Plant Physiol. 46, 406–411 (1970)

    Google Scholar 

  • Wilson, D. B., Hogness, D. S.: UDP-galactose 4-epimerase. In: Methods in enzymology, Neufeld, E. F., Ginsburg, V., eds., vol. 8, p. 235–240. New York and London: Academic Press 1966

    Google Scholar 

  • Yamada, M.: Studies on fat metabolism in germinating castor beans. IV. Glycerol metabolism. Sci. Pap. Coll. Gen. Educ. Univ. Tokyo 10, 283–323 (1960)

    Google Scholar 

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  1. Botanisches Institut, Universität Köln, Gyrhofstraße 15, D-5000, Köln 41, Federal Republic of Germany

    B. Königs & E. Heinz

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  1. B. Königs
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  2. E. Heinz
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Königs, B., Heinz, E. Investigation of some enzymatic activities contributing to the biosynthesis of galactolipid precursors in Vicia faba . Planta 118, 159–169 (1974). https://doi.org/10.1007/BF00388391

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  • DOI: https://doi.org/10.1007/BF00388391

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