Skip to main content
SpringerLink
Log in

Fluxes and compartmentation of potassium and chloride in the green alga Mougeotia

  • Published:
Planta Aims and scope Submit manuscript

Summary

Some ionic relations of the filamentous green alga Mougeotia sp. have been analyzed under different light conditions. Data from influx and efflux measurements using 86Rb+ and 36Cl- fit the model of three cellular compartments (cell wall, cytoplasm, vacuole) in series. This result is remarkable, since in a Mougeotia cell at least two thirds of the cytoplasmic compartment are occupied by the cell-filling, flat and nearly rectangular chloroplast which is axially oriented. The chloroplast is concluded to be part of the cytoplasmic flux compartment.

Photosynthetically saturating irradiances of continuous white light enhance the active and passive fluxes of K+ and Cl- at the plasmalemma by a factor of 3. Photosystem II is responsible for the light-dependent increase of the uptake of Cl- (36Cl-) whereas the uptake of K+ (86Rb+) depends additionally on energy from photosystem I.

Ion flux measurements performed after irradiations with red and far-red, respectively, show that the fluxes of K+ and Cl- across the plasmalemma are not affected by the state of phytochrome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barber, J.: The efflux of potassium from Chlorella pyrenoidosa. Biochim. biophys. Acta (Amst.) 163, 531–538 (1968)

    Google Scholar 

  • Bentrup, F. W.: Zur Funktion der Zellmembran bei der Cytomorphogenese. Ber. dtsch. bot. Ges. 81, 311–314 (1968)

    Google Scholar 

  • Briggs, W. R., Rice, H. V.: Phytochrome: Chemical and physical properties and mechanism of action. Ann. Rev. Plant Physiol. 23, 293–334 (1972)

    Article  Google Scholar 

  • Cram, W. J.: Compartmentation and exchange of chloride in carrot root tissue. Biochim. biophys. Acta (Amst.) 163, 339–353 (1968)

    Google Scholar 

  • Cram, W. J.: Chloride fluxes in cells of the isolated root cortex of Zea mays. Aust. J. biol. Sci. 26, 757–779 (1973)

    Google Scholar 

  • Filippis, L. F. de, Pallaghy, C. K.: Effect of light on the volume and ion relations of chloroplasts in detached leaves of Elodea densa. Aust. J. biol. Sci. 26, 1251–1265 (1973)

    Google Scholar 

  • Gimmler, H., Ullrich, W., Domanski-Kaden, J., Urbach, W.: Excretion of glucolate during synchronous culture of Ankistrodesmus braunii in the presence of disalicylidenepropanediamine or hydroxypyridinemethanesulfonate. Plant and Cell Physiol. 10, 103–112 (1969)

    Google Scholar 

  • Haupt, W.: Die Orientierung der Phytochrom-Moleküle in der Mougeotiazelle: Ein neues Modell zur Deutung der experimentellen Befunde. Z. Pflanzenphysiol. 58, 331–346 (1968)

    Google Scholar 

  • Haupt, W., Schönbohm, E.: Light-oriented chloroplast movements. In: Photobiology of microorganisms, P. Halldal, ed., pp. 283–307. New York-London-Sydney-Toronto: Wiley-Interscience 1970

    Google Scholar 

  • Hope, A. B.: Ion transport and membranes. A biophysical outline. London: Butterworth 1971

    Google Scholar 

  • Larkum, A. W.: Ionic relations of chloroplasts in vivo. Nature (Lond.) 218, 447–449 (1968)

    Google Scholar 

  • MacRobbie, E. A. C.: The active transport of ions in plant cells. Quart. Rev. Biophys. 3, 251–294 (1970)

    Google Scholar 

  • MacRobbie, E. A. C.: Fluxes and compartmentation in plant cells. Ann. Rev. Plant Physiol. 22, 75–96 (1971)

    Article  Google Scholar 

  • MacRobbie, E. A. C., Dainty, J.: Sodium and potassium distribution and transport in the seaweed Rhodymenia palmata (L.). Grev. Physiol. Plantarum (Kbh.) 11, 782–801 (1958)

    Google Scholar 

  • Nobel, P. S.: Light-induced changes in the ionic content of chloroplasts in Pisum sativum. Biochim. biophys. Acta (Amst.) 172, 134–143 (1969)

    Google Scholar 

  • Pierce, W. S., Higinbotham, N.: Compartments and fluxes of K+, na+ and Cl- in Avena coleoptile cells. Plant Physiol. 46, 666–673 (1970)

    Google Scholar 

  • Pitman, M. G.: The determination of the salt relations of the cytoplasmic phase in cells of beetroot tissue. Aust. J. biol. Sci. 16, 647–668 (1963)

    Google Scholar 

  • Rasmussen, H.: Cell communication, calcium ion and cyclic adenosine monophosphate. Science 170, 404–412 (1970)

    PubMed  Google Scholar 

  • Raven, J. A.: Action spectra for photosynthesis and light-stimulated ion transport processes in Hydrodictyon africanum. New Phytologist 68, 45–62 (1969a)

    Google Scholar 

  • Raven, J. A.: Effects of inhibitors on photosynthesis and the active influxes of K and Cl in Hydrodictyon africanum. New Phytologist. 68, 1089–1113 (1969b)

    Google Scholar 

  • Satter, R. L., Marinoff, P., Galston, A. W.: Phytochrome-controlled nyctinasty in Albizzia julibrissin. II. Potassium flux as a basis for leaflet movement. Amer. J. Bot. 57, 916–926 (1970)

    Google Scholar 

  • Schäfer, E., Schmidt, W., Mohr, H.: Comparative measurements of phytochrome in cotyledons and hypocotyl hook of mustard (Sinapis alba L.). Photochem. Photobiol. 18, 331–334 (1973)

    Google Scholar 

  • Sweet, H. C., Hillman, W. S.: Phytochrome control of nyctinasty in Samanea as modified by oxygen, submergence and chemicals. Physiol. Plantarum (Kbh.) 22, 776–786 (1969)

    Google Scholar 

  • Umbreit, W. W., Burris, R. H., Stauffer, J. F.: Manometric techniques and tissue metabolism. Minneapolis: Burgess Publ. Co. 1951

    Google Scholar 

  • Wagner, G.: Ionenflüsse und Phytochrom-abhängige Chloroplastenbewegung bei Mougeotia spec. Doct. dissert. Univ. Tübingen, W.-Germany (1974)

    Google Scholar 

  • Wagner, G., Haupt, W., Laux, A.: Reversible inhibition of chloroplast movement by cytochalasin B in the green alga Mougeotia. Science 176, 808–809 (1972)

    PubMed  Google Scholar 

  • Weisenseel, M.: Vergleichende Untersuchungen zum Einfluß der Temperatur auf lichtinduzierte Chloroplastenverlagerungen. I. Die Wirkung verschiedener Licht-intensitäten auf die Chloroplastenanordnung und ihre Abhängigkeit von der Temperatur. Z. Pflanzenphysiol. 59, 56–69 (1968)

    Google Scholar 

  • Weisenseel, M., Smeibidl, E.: Phytochrome controls the water permeability in Mougeotia. Z. Pflanzenphysiol. 70, 420–431 (1973)

    Google Scholar 

  • Winocur, B. A., Macey, F. I., Tolberg, A. B.: Electrolyte exchange in isolated spinach chloroplasts. Biochim. biophys. Acta (Amst.) 150, 32–40 (1968)

    Google Scholar 

  • Yunghans, H., Jaffe, M. J.: Rapid respiratory changes due to red light or acetylcholine during the early events of phytochrome-mediated photomorphogenesis. Plant Physiol. 49, 1–7 (1972)

    Google Scholar 

  • Ziegler, R., Egle, K.: Zur quantitativen Analyse der Chloroplastenpigmente. Beitr. Biol. Pflanzen 41, 11–37 (1965)

    Google Scholar 

Download references

Author information

Author notes

  1. Gottfried Wagner

    Present address: Botanisches Institut der Universität, Schloßgarten 4, D-8520, Erlangen, Federal Republic of Germany

Authors and Affiliations

  1. Institut für Biologie I der Universität, Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany

    Gottfried Wagner

Authors
  1. Gottfried Wagner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wagner, G. Fluxes and compartmentation of potassium and chloride in the green alga Mougeotia . Planta 118, 145–157 (1974). https://doi.org/10.1007/BF00388390

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388390

Keywords

Search

Navigation