Summary
Rapid freezing of higher plant tissue in liquid nitrogen renders the cells permeable to a wide range of substrate molecules. Tissue permeabilized by repeated freeze-thaw treatment can be used for the measurement of several enzymes. With most of the tissues examined maximum in vivo activities were obtained using a combination of freeze-thaw treatment followed by vacuum infiltration. The activities and properties of enzymes determined with this procedure are very comparable with those obtained for enzymes prepared by conventional extraction procedures.
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Abbreviations
- DPT:
-
Thiamin pyrophosphate
- FMN:
-
Flavin mononucleotide
- NAD:
-
nicotinamide adenine dinucleotide
References
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D. Rhodes was supported by a Science Research Council Studentship.
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Rhodes, D., Stewart, G.R. A procedure for the in vivo determination of enzyme activity in higher plant tissue. Planta 118, 133–144 (1974). https://doi.org/10.1007/BF00388389
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DOI: https://doi.org/10.1007/BF00388389