Summary
If the 1000×g supernatant from a homogenate of etiolated pea (Pisum sativum L. cv. Alaska) epicotyls is briefly irradiated with red light at ca. 0° C, the increase of phytochrome content in the 1000–7000×g particulate fraction is strongly dependent upon the Ca2+concentration, being highest at 10 mM. This Ca2+-dependent binding of phytochrome to the particulate fraction in the supernatant is partially red/far-red reversible. Mg2+ is significantly less effective than Ca2+ while Na+ and K+ have no effect.
The 80 000×g supernatant (S) and the 1000–7000×g particulate fraction (P) were prepared and separated from a pea homogenate. When either S or P were briefly exposed to red light and then mixed together at 0° C in darkness and the presence of 10 mM Ca2+, the amount of phytochrome bound to the 7000×g pellet increased immediately. This increase was 30% after mixing irradiated S with dark P, 18% after mixing irradiated P with dark S and 55% after both S and P had been irradiated and mixed. In all cases, the red light effect is totally far-red reversible.
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Abbreviations
- R:
-
red
- FR:
-
far-red
- Pr:
-
red-absorbing form of phytochrome
- Pfr:
-
far-red-absorbing form of phytochrome
- S:
-
supernatant
- P:
-
particulate fraction
Reference
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Yamamoto, K.T., Furuya, M. Photoreversible binding in vitro of cytosolic phytochrome to particulate fraction isolated from pea epicotyls. Planta 127, 177–186 (1975). https://doi.org/10.1007/BF00388379
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DOI: https://doi.org/10.1007/BF00388379