Skip to main content
SpringerLink
Log in

Studies on the secretion of maize root-cap slime

III. Histochemical and autoradiographic localization of incorporated fucose

  • Published:
Planta Aims and scope Submit manuscript

Summary

Autoradiography was used to localize the sites of incorporation of L-[3H]fucose into root tips of maize (Zea mays L. cv. S.X. 17). By light microsocpy, accumulation of label from [3H]fucose could be seen in the peripheral cells of the root cap. Extraction of sections prepared by freeze-substitution showed that most of the label in the cytoplasm of peripheral root-cap cells is water-soluble whereas label associated with the wall is sodiumhydroxide-soluble. In the electron microscope, glutaraldehyde-fixed peripheral cells of maize root caps are characterized by the presence of numerous dictyosomes and vesicles. The distended dictyosome cisternae and vesicles have large deposits of silver after staining with periodic acid-silver methanamine. An accumulation of material similar to that found in dictyosomes and vesicles is observed between the cell membrane and wall in glutaraldehyde-formaldehyde-fixed tissue. At the electron-microscope level label in peripheral root cap cells incubated in [3H]fucose for periods from 10 to 120 min was found primarily over dictyosomes and dictyosome vesicles. In pulse-chase experiments label was chased from the dictyosomes and vesicles to the exterior of the cell in 20–30 min. Less than 19% of the label in pulse-chase experiments was associated with organelles other than dictyosomes or dictyosome vesicles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bekesi, J. G., Winzler, R. J.: The metabolism of plasma glycoproteins: Studies on the incorporation of L-fucose 1-14C into tissue and serum in the normal rat. J. biol. Chem. 242, 3873–3879 (1967)

    PubMed  Google Scholar 

  • Bennett, G., LeBlond, C. P.: Formation of cell coat material for the whole surface of columnar cells in the rat small intestine, as visualized by radiography with fucose-13H. J. Cell Biol. 46, 409–416 (1970)

    Article  PubMed  Google Scholar 

  • Bowles, D. J., Northcote, D. H.: The sites of synthesis and transport of extracellular polysaccharides in the root tissue of maize. High resolution autoradiography. I. Methods. J. Cell Biol. 15, 173–188 (1962)

    Google Scholar 

  • Dauwalder, M., Whaley, W. G.: 1974. Patterns of incorporation of 3H galactose by cells of Zea mays root tips. J. Cell Sci. 14, 11–27 (1974)

    PubMed  Google Scholar 

  • Haddad, A., Smith, M. D., Herocovics, A., Nadler, N. J., LeBlond, C.P.: Radioautographi study of in vivo and in vitro incorporation of fucose [3H] into thyroglobulin by rat thyroid follicular cells. J. Cell Biol. 49, 856–882 (1971)

    Article  PubMed  Google Scholar 

  • Harris, P. J., Northcote, D. H.: Patterns of polysaccharide biosynthesis in differentiating cells of maize root tips. Biochem. J. 120, 479–491 (1970)

    PubMed  Google Scholar 

  • Jensen, W. A.: The composition of the developing primary wall in onion root tip cells. II. Cytochemical localizations. Amer. J. Bot. 47, 287–295 (1960)

    Google Scholar 

  • Jensen, W. A., Ashton, M.: Composition of developing primary wall in onion root tip cells. I. Quantitative analysis. Plant Physiol. 35, 313–323 (1960)

    Google Scholar 

  • Jones, D. D., Morré, D. J.: Golgi apparatus mediated polysaccharide secretion by outer root cap cells of Zea mays. II. Isolation and characterization of the secretory product. Z. Pflanzenphysiol. 56, 166–169 (1967)

    Google Scholar 

  • Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmalality for use in electron microscopy. J. Cell. Biol. 27, 137 A (1965)

    Google Scholar 

  • Kirby, E. G., Roberts R. M.: The localized incorporation of H3-1-fucose into cell wall polysaccharides of the cap and epidermis of corn roots: Autoradiographic and biochemical studies. Planta (Berl.) 99, 211–221 (1971)

    Google Scholar 

  • Mollenhauer, H. H.: Transition forms of golgi apparatus in secretion vesicles J. Ultrastruct. Res. 12, 439–446 (1965)

    PubMed  Google Scholar 

  • Mollenhauer, H. H., Whaley, W. G., Leech, J. H.: A function of the golgi apparatus in outer root cap cells. J. Ultrastruct. Res. 5, 193–200 (1961)

    PubMed  Google Scholar 

  • Morré, D. J., Jones, D. D., Mollenhauer, H. H.: Golgi apparatus mediated polysaccharide secretion by outer root cap cells of Zea mays. Planta (Berl.) 74, 286–301 (1967)

    Google Scholar 

  • Northcote, D. H., Pickett-Heaps, J. D.: A function of the golgi apparatus in polysaccharide synthesis and transport in the root cap cells of wheat. Biochem. J. 98, 159–167 (1966)

    PubMed  Google Scholar 

  • O'Brien, T. P.: The cytology of cell wall formation in some eukaryotic cells. Bot. Rev. 38, 87–118 (1972)

    Google Scholar 

  • Parkhouse, R. M. E., Melchers, F.: Biosynthesis of the carbohydrate portions of immunoglobulin M. Biochem. J. 125, 235–240 (1971)

    PubMed  Google Scholar 

  • Paull, R. E., Johnson, C. M., Jones, R. L.: Studies on the secretion of maize root cap slime. I. Some properties of the secreted polymer. Plant Physiol., 1975

  • Paull, R. E., Jones, R. L.: Studies on the secretion of maize root cap slime. II. Localization of slime production. Plant Physiol., 1975

  • Pickett-Heaps, J. D.: The use of radioautography for investigating secretions in plant cells. Protoplasma 64, 49–66 (1967a)

    Google Scholar 

  • Pickett-Heaps, J. D.: Preliminary attempts at ultrastructural polysaccharide localization in root tip cells. J. Histochem. Cytochem. 15, 442–455 (1967b)

    PubMed  Google Scholar 

  • Pickett-Heaps, J. D.: Further observations on the golgi apparatus and its function in cells of the wheat seedling. J. Ultrastruct. Res. 18, 287–303 (1967c)

    PubMed  Google Scholar 

  • Pickett-Heaps, J. D., Northcote, D. H.: Relationship of cellular organelles to the formation and development of the plant cell wall. J. exp. Bot. 17, 20–26 (1966)

    Google Scholar 

  • Rambourg, A.: An improved silver methenamine technque for the detection of periodicacid reactive complex carbohydrates with the electron microscope. J. Histochem. Cytochem. 15, 409–412 (1967)

    PubMed  Google Scholar 

  • Renyolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Article  PubMed  Google Scholar 

  • Roberts, R. M., Butt, V. S.: Patterns of incorporation of D-galactose in cell wall polysaccharides in growing maize roots. Planta 84, 250–262 (1969)

    Google Scholar 

  • Schnepf, E.: Physiologie und Morphologie sekretorischer Pflanzenzellen. In: Sekretion und Exkretion, p. 72–78, Berlin-Heidelberg-New York; Springer 1965)

    Google Scholar 

  • Sturgess, J. M., Minakes, E., Mitranic, M. M., Moscarello, M. A.: The incorporation of L-fucose into glycoproteins in the golgi apparatus of rat livers and in serum. Biochem. biophys. acta (Amst.) 320, 123–132 (1973)

    Google Scholar 

  • Whaley, W. G., Kephart, J. E., Mollenhauer, H. H.: Developmental changes in Golgi apparatus of maize root cells Amer. J. Bot. 46, 743–751 (1959)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, University of California, 94720, Berkeley, California, USA

    Robert E. Paull & Russell L. Jones

Authors
  1. Robert E. Paull
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Russell L. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

I and II=Paull et al., 1975; Paull and Jones, 1975.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paull, R.E., Jones, R.L. Studies on the secretion of maize root-cap slime. Planta 127, 97–110 (1975). https://doi.org/10.1007/BF00388371

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388371

Keywords

Search

Navigation