Abstract
Cloned, cytokinin-habituated tissues of Nicotiana tobacum L. cv. “Havana 425” are able to grow in culture at 25° C without added cytokinin. These tissues vary in their expression of the habituated phenotype at 16° C. When cytokinin-requiring pith tissues are converted to the habituated state by 35° C treatment, all of the habituated cells are cold sensitive. After several transfers in culture, some of these habituated cells give rise to stable, cold resistant variants. Both phenotypes are inherited by individual cells. Cold sensitive clones at 16° C and non-habituated clones at 16° C as well as 25° C show the same dose response to the cytokinin, kinetin. This suggests that at the physiological level, cold sensitivity results from a decreased production of cell division factors rather than from a decreased affinity of cellular receptors for these factors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- CDF:
-
Cell division factor(s)
- NAA:
-
α-naphthalencacetic acid
References
Bergmann, L.: Growth and division of single cells of higher plants in vitro. J. Gen. Physiol. 43, 841–851 (1960)
Binns, A., Meins, F., Jr.: Evidence that habituation of tobacco pith cells for cell division-promoting factors is heritable and potentially reversible. Proc Nat. Acad. Sci. (USA) 70, 2660–2662 (1973)
Dyson, W.H., Hall, R.H.: N6-(Δ 2-Isopentenyl) adenosine: its occurrence as a free nucleoside in a autonomous strain of tobacco tissue. Plant Physiol. 50, 616–621 (1972)
Einset, J.W., Skoog, F.: Biosynthesis of cytokinin in cytokinin-autotrophic tobacco callus. Proc. Nat. Acad. Sci. (USA) 70, 658–660 (1973)
Fox, J.E.: Growth factor requirements and chromosome number in tobacco tissue cultures. Physiol. Plant. 16, 793–803 (1963)
Gibbs, J.L., Dougall, D.K.: The growth of single cells from Nicotiana tabacum callus tissue in nutrient medium contaiuing agar. Exp. Cell Res. 40, 85–95 (1965)
Linsmaier, E.M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127 (1965)
Lutz, A.: Aptitudes morphogénétiques des cultures de tissus d'origine unicellulaire. In: Les cultures de tissus de plantes, pp. 163–168. Colloq. Int. C. N. R. S. No. 193. Paris: CNRS 1971
Meins, F., Jr.: Regulation of phenotypic expression in crown-gall teratoma tissues of tobacco. Develop. Biol. 24, 287–300 (1971)
Meius, F., Jr.: Mcchanisms underlying the persistence of tumour antonomy in crown-gall disease. In: Tissue culture and plant science pp. 233–264, Street, H.E., ed. London: Academic Press 1974
Meins, F., Jr.: Reversal of the neoplastic state in plants. Am. J. Pathol. 89, 687–702 (1977)
Meins, F., Jr., Binns, A.: Epigenetic variation of cultured somatic cells: Evidence for gradual changes in the requirement for factors promoting cell division. Proc. Nat. Acad. Sci. (USA) 74, 2928–2932 (1977)
Meins, F., Jr., Binns, A.N.: Epigenetic clonal variation in the requirement of plant cells for cytokinins. In: The clonal basis for development. pp. 185–201, Subtelny, S., Sussex, I.M., eds. New York: Academic Press 1978
Syōno, K., Furuya, T.: Effect of temperature on the cytokinia requirements of tobacco calluses. Plant Cell Physiol. 12, 61–71 (1971)
Tandeau de Marsac, N., Jouanneau, J.-P.: Variation de l'exigence en cytokinine de ligneés clonales de cellules de tabac. Physiol Vég. 10, 369–380 (1972)
Wood, H.N., Braun, A.C., Brandes, H., Kende, H.: Studies on the distribution and properties of a new class of cell division-promoting substances from higher plant species. Proc. Nat. Acad. Sci. USA 62, 349–356 (1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Binns, A.N., Meins, F. Cold-sensitive expression of cytokinin habituation by tobacco pith cells in culture. Planta 145, 365–369 (1979). https://doi.org/10.1007/BF00388362
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388362