Abstract
The selective action of the antibiotics chloramphenicol and cycloheximide on the synthesis of ferredoxin in liquid cultures of Chlamydomonas reinhardii was studied. Highly specific antibodies raised against Chlamydomonas ferredoxin were used to determine the in vivo synthesis of apoferredoxin and conversion into native protein. The results indicate that 80S ribosomes are involved in the synthesis. Chlamydomonas cells growing in the absence of iron did not synthesize immunologically detectable amounts of ferredoxin. We suggest that this is based upon feed-back inhibition of apoferredoxin synthesis at the translational level.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- CAP:
-
chloramphenicol
- CHI:
-
cycloheximide
- IgG:
-
Immunoglobulin G
- PBS:
-
140.4 mM NaCl. 9 mM Na2HPO4, 1.3 mM NaH2PO4 (pH 74)
- SDS:
-
sodium dodecvl sulphate
- Fd:
-
Ferredoxin
- apoFd:
-
Apoferredoxin
- CM-Fd:
-
Scarboxymethylated Fd
- TCA-Fd:
-
Fd treated with trichloroacetic acid
References
Armstrong, J.J., Surzycki, S.J., Moll, B., Levine, R.P.: Genetic Transcription and Translation Specifying Chloroplast Components in Chlamydomonas reinhardii. Biochemistry 10, 692–701 (1971)
Beinert, H.: Iron-Sulfur Protein, vol. I, pp. 1–38, Lovenberg, W., ed. New York: Academic Press 1975
Buchanan, B.B., Arnon, D.I.: Ferredoxin chemistry and function in photosynthesis nitrogen fixation and fermentative metabolism. Adv. Enzymol. 33, 119–176 (1970)
Huisman, J.G., Gebbink, M.G.Th., Modderman, P., Stegwee, D.: The Coding-Site of chloroplast Ferredoxin. Planta 137, 97–105 (1977a)
Huisman, J.G., Bernards, A., Liebregts, P., Gebbink, M.G.Th., Stegwee, D.: Qualitative and Quantitative Immunofluorescence Studies of Chloroplast Ferredoxin. Planta 137, 279–286 (1977b)
Huisman, J.G., Stapel, S., Gebbink, M.G.Th.: Characterization of Ferredoxin on a nanomole Scale. Anal. Biochem. 90, 501–509 (1978a)
Huisman, J.G., Stapel, S., Muijsers, A.O.: Two Different Plant Type Ferredoxins in Each of Two Petunia Species. FEBS Lett. 85, 198–202 (1978b)
Keresztes-Nagy, S., Perini, F., Margoliash, E.: Preparation and characterization of alfalfa ferredoxin. J. Biol. Chem. 241, 5955–5966 (1966)
Mancini, G., Carbonara, A.O., Heremans, J.F.: Immunochemical Quantitation of Antigens by Single Radial Immunodiffusion. Immunochemistry 2, 235–254 (1965)
Matson, R.S., Kimura, T.: Ferredoxin Biosynthesis in Euglena gracilis. Biochim. Biophys. Acta. 442, 76–87 (1976)
Sluiters-Scholten, C.M.Th., Moll, W.A.W., Stegwee, D.: Ferredoxin and Ferredoxin-NADP-oxidoreductase in Leaves of Phaseolus vulgaris L. Planta 133, 289–294 (1977)
Smillie, R.M.: Isolation of Phytoflavin, A Flavoprotein with Chloroplast Ferredoxin Activity. Plant Physiol. 40, 1124–1128 (1965)
Surzycki, S.: Algal-Cultures-Sources and Methods of Cultivation. In: Methods in Enzymology, vol. XXIII, pp. 67–73, San Pietro, A., ed. New York, London: Academic Press 1971
Tomati, U., Matarese, R., Frederici, G.: Feiredoxin Activation by Rhodanese. Phytochemistry 13, 1703–1706 (1974)
Trakatellis, A.C., Schwartz, G.: The Biosynthesis of Ferredoxin in a Cell-free System. Proc. Nat. Acad. Sci. (USA) 63, 436–441 (1969)
Zähringer, J., Baliga, B.S., Munro, H.N.: Novel mechanism for translation control in regulation of ferritin synthesis by iron. Proc. Nat. Acad. Sci. (USA), 73, 857–861 (1976)
Zumft, W.G., and Spiller, H.: Characterization of a Flavodoxin from the Green Algae Chlorella. Biochem. Biophys. Res. Commun. 45, 112–118 (1971)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huisman, J.G., Touw, I., Liebregts, P. et al. Biosynthesis of ferredoxin in Chlamydomonas reinhardii . Planta 145, 351–356 (1979). https://doi.org/10.1007/BF00388360
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388360