Skip to main content

A radioimmunoassay for abscisic acid

Planta volume 146, pages 139–145 (1979)Cite this article

Abstract

We have developed a radioimmunoassay (RIA) for abscisic acid (ABA) in the 0.1 ng to 2.5 ng range. Antibodies were obtained from rabbits immunized with ABA bound via its carboxyl group to bovine serum albumin. Cross-reactivity studies indicate that ABA esters are completely cross-reactive with ABA, while trans, trans abscisic acid (t-ABA) phaseic acid (PA) and dihydrophaseic acid (DPA) have much lower but significant cross-reactivities. Purification methods which reduce the levels of cross-reacting substances are described.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Abbreviations

RIA:

radioimmunoassay

DPA:

4′-dihydrophaseic acid

PA:

phaseic acid

GC:

gas chromatography

HPLC:

high performance liquid chromatography

TLC:

thin-layer chromatography

BSA:

bovine serum albumin

ABA:

abscisic acid

t-ABA:

trans, trans abscisic acid

IAA:

indoleacetic acid

References

  • Ciha, A.J., Brenner, M.L., Brun, W.A.: Rapid separation and quantification of abscisic acid from plant tissues using high performance liquid chromatography. Plant Physiol. 59, 821–826 (1977)

    Google Scholar 

  • Fuchs, Y., Mayak, S., Fuchs, S.: Detection and quantitative determination of abscisic acid by immunological assay. Planta 103, 117–125 (1972)

    Google Scholar 

  • Gillard, D.F., Walton, D.C.: Abscisic acid metabolism by a cell-free preparation from Echinocystis lobata liquid endosperm. Plant Physiol. 58, 790–795 (1976)

    Google Scholar 

  • Harrison, M.A., Walton, D.C.: Abscisic acid metabolism in water-stressed bean leaves. Plant Physiol. 56, 250–254 (1975)

    Google Scholar 

  • Hunter, W.M.: Radioimmunoassay. In: Handbook of Experimental Immunology, pp. 17.1–17.36, Weir, D.M., ed. Oxford: Blackwell Scientific Publications 1973

    Google Scholar 

  • Jones, W.W., Coggins, C.W., Embleton, T.W.: Endogenous abscisic acid in relation to bud growth in alternate bearing ‘Valencia’ orange. Plant Physiol. 58, 681–682 (1976)

    Google Scholar 

  • Kesser, W.W.: Rapid isolation of antigens from cells with Staphylococcal protein A-antibody adsorbent. I. Immunol. 115, 1617–1624 (1975)

    PubMed  Google Scholar 

  • Leshem, Y., Philosoph, S., Wurzburger, J.: Glycosylation of free trans, trans abscisic acid as a contributing factor in bud dormancy break. Biochem. Biophys. Res. Commun. 57, 526–531 (1974)

    PubMed  Google Scholar 

  • Loveys, B.R., Kriedemann, P.E.: Internal control of stomatal physiology and photosynthesis. I. Stomatal regulation and associated changes in endogenous levels of abscisic and phaseic acids. Aust. J. Plant Physiol. 1, 407–415 (1974)

    Google Scholar 

  • Milborrow, B.V.: The metabolism of abscisic acid. J. Exp. Bot. 21, 17–29 (1970)

    Google Scholar 

  • Milborrow, B.V.: The chemistry and physiology of abscisic acid. Ann. Rev. Plant Physiol. 25, 259–307 (1974)

    Article  Google Scholar 

  • Niswender, G.D., Akbar, A.M., Nett, T.M.: Use of specific antibodies for quantification of steroid hormones. Methods Enzymol. 36, 16–34 (1975)

    PubMed  Google Scholar 

  • Pengelly, W., Meins, F.: A specific radioimmunoassay for nanogram quantities of the auxin indole-3-acetic acid. Planta 136, 173–180 (1977)

    Google Scholar 

  • Seeley, S.D., Powell, L.E.: Electron capture-gas chromatography for sensitive assay of abscisic acid. Anal. Biochem. 35, 530–533 (1970)

    PubMed  Google Scholar 

  • Sondheimer, E., Walton, D.C.: Structure-activity correlations with compounds related to abscisic acid. Plant Physiol. 45, 244–248 (1970)

    PubMed  Google Scholar 

  • Sondheimer, E., Galson, E.C., Chang, Y.P., Walton, D.C.: Asymmetry, its importance to the action and metabolism of abscisic acid. Science 174, 829–831 (1971)

    Google Scholar 

  • Steiner, A.L., Kipnis, D.M., Utiger, R., Parker, C.: Radioimmunoassay for the measurement of adenosine 3′,5′-cyclic phosphate. Proc. Nat. Acad. Sci. USA 64, 367–373 (1969)

    PubMed  Google Scholar 

  • Walton, D.C., Sondheimer, E.: Activity and metabolism of 14C-(±)-abscisic acid derivatives. Plant Physiol. 49, 290–292 (1972)

    Google Scholar 

  • Walton, D.C., Dorn, B., Fey, J.: The isolation of an abscisic acid metabolite, 4′-dihydrophaseic acid, from non-imbibed Phaseolus vulgaris seed. Planta 112, 87–90 (1973)

    Google Scholar 

  • Walton, D.C., Galson, E.C.: A radioimmunoassay for ABA (Abstr.). Plant Physiol. 59 (Suppl.), 77 (1977)

    Google Scholar 

  • Walton, D.C., Wellner, R., Horgan, R.: Synthesis of tritiated abscisic acid of high specific activity. Phytochemistry 16, 1059–1061 (1977)

    Article  Google Scholar 

  • Weiler, E.W., Zenk, M.H.: Radioimmunoassay for the determinations of digoxins and related compounds in Digitalis lanata. Phytochemistry 15, 1537–1545 (1976)

    Article  Google Scholar 

  • Weiler, E.W.: Radioimmunoassay for the determination of free and conjugated abscisic acid. Planta 144, 255–263 (1979)

    Google Scholar 

  • Zeevaart, J.A.D., Milborrow, B.V.: Metabolism of abscisic acid and the occurrence of epi-dihydrophaseic acid in Phaseolus vulgaris. Phytochemistry 15, 493–500 (1976)

    Article  Google Scholar 

  • Zeevaart, J.A.D.: Sites of abscisic acid synthesis and metabolism in Ricinus communis L. Plant Physiol. 59, 788–791 (1977)

    Google Scholar 

Download references

Author information

Affiliations

  1. Department of Environmental and Forest Biology, State University of New York, College of Environmental Science and Forestry, 13210, Syracuse, NY, USA

    Daniel Walton, William Dashek & Eva Galson

Authors
  1. Daniel Walton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William Dashek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eva Galson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Walton, D., Dashek, W. & Galson, E. A radioimmunoassay for abscisic acid. Planta 146, 139–145 (1979). https://doi.org/10.1007/BF00388224

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388224

Key words

  • Abscisic acid
  • Radioimmunoassay (ABA)