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Repair of functional and ultrastructural alterations after thermal injury of Physarum polycephalum

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Abstract

Repair of thermal injury of Physarum polycephalum Schw. plasmodia has been studied by light and electron microscopy. As a result of heating the plasmodia for 10 min at 42°C both the unordered and shuttle protoplasmic streaming were arrested; the outer plasmodial membrane showed alterations at sites of contact with water; the onset of the next mitosis was considerably delayed. The plasmodial ultrastructure was markedly disturbed, including disappearance of the granular component of the nucleoili and a compact, almost fibrillar structure of the latter. The mitochondria became distorted and their intracristal spaces enlarged while the outer and inner membranes appeared in some places to be separated. Glycogen particles disappeared from the cytoplasm. Recovery of both types of protoplasmic streaming of the motility of the plasmodium, of the resistance of its membrane to contact with water, and of the ability of the organism to go through the cell cycle went all hand in hand with the normalization of the structure of nucleoli, mitochondria and cytoplasm. All of the functional and structural characteristics are normalized within ca. 9 h following heating.

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  1. Laboratory of Cytophysiology and Cytoecology, V.L. Komarov Botanical Institute, Academy of Sciences of the USSR, 197022, Leningrad P-22, USSR

    A. G. Lomagin

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Lomagin, A.G. Repair of functional and ultrastructural alterations after thermal injury of Physarum polycephalum . Planta 142, 123–134 (1978). https://doi.org/10.1007/BF00388203

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  • DOI: https://doi.org/10.1007/BF00388203

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