Abstract
Chromatographic detection of particles with 0.05 to 10.0 μm in diameter by using a capillary tube is reported. Retention times of particles depended on their diameter. Complete peak separation was observed in the chromatogram of a mixture of particles with 0.05 and 10.0 μm in diameter. The application of this technique as detecting method for contaminants in a bioreactor is considered.
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Abbreviations
- c wt%:
-
concentration
- D p μm:
-
diameter of particle
- Q cm3/min:
-
flow rate
- t r min:
-
retention time
References
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Brough, A. W. J.; Hillman, D. E.; Perry, R. W.: Capillary hydrodynamic chromatography — an investigation into operational characteristics. J Chromatogr. 208 (1981) 175–182
Tijssen, R.; Bos, J.; van Kreveld, M. E.: Hydrodynamic chromatography of macromolecules in open microcapillary tubes. Anal. Chem. 58 (1986) 3036–3044
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Shiragami, N. A simple and rapid method for the detection of contaminants in a bioreactor. Bioprocess Engineering 5, 115–117 (1990). https://doi.org/10.1007/BF00388189
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DOI: https://doi.org/10.1007/BF00388189