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Primer dependent and independent forms of soluble starch synthetase from developing barley endosperms

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Abstract

The activity of soluble starch synthetase (ADP-glucose: α-1,4-glucan α-4-glucosyltransferase) in the non-purified extract from 16 day-old Bomi barley endosperms (Hordeum vulgare L.) was low and the reaction was non-linear when plotted against protein concentration. Starch synthetase was purified by ammonium sulfate precipitation and DEAE-cellulose chromatography and separated into four fractions. In the absence of an added carbohydrate primer two of the four fractions catalized the synthesis of a methanol-precipitable α-glucan when high concentrations of sodium citrate and bovine serum albumim were added. The rate of α-glucan synthesis by the unprimed reaction was higher than for the primed reaction. The four enzyme fractions were active with ADP-Glc, but not with UDP-Glc, both in the primed and in the unprimed reaction.

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Abbreviations

ADP-Glc:

adenosine diphosphate glucose

DEAE-cellulose:

diethylaminoethyl-cellulose

DDT:

dithiothreitol

EDTA:

ethylenediaminetetraacetic acid

GSH:

glutathione

kat:

katal (1 mol s-1)

TRIS:

tris-(hydroxymethyl)-aminomethane

UDP-Glc:

uridine diphosphate glucose

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Author notes

  1. Martin Kreis

    Present address: Biochemistry Department, Rothamsted Experimental Stations, AL5 2JQ, Harpenden, Herts, UK

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  1. Risø National Laboratory, Agricultural Research Department, DK-4000, Roskilde, Denmark

    Martin Kreis

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  1. Martin Kreis
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Kreis, M. Primer dependent and independent forms of soluble starch synthetase from developing barley endosperms. Planta 148, 412–416 (1980). https://doi.org/10.1007/BF00388131

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  • DOI: https://doi.org/10.1007/BF00388131

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