Abstract
Dwarf french beans, Phaseolus vulgaris L., were grown with or without inoculation with rhizobia (strain 3644), and with or without a combined nitrogen source (nitrate or ammonium ions). The distribution of radioactivity into products of dark 14CO2 assimilation was studied in roots or nodules from these plants. A detailed study was also made of the distribution and rates of excretion of nitrogen in xylem bleeding sap in 28 day old plants grown on the various sources of nitrogen. Whereas detached nodules accumulated radioactive glycine, serine and glutamate when incubated with 14CO2, bleeding sap from plants root fed 14CO2 contained low levels of radioactivity in these compounds but higher levels in allantoin. Chemical analysis showed allantoin to be the major compound transported in the xylem of nodulated plants, whether or not they were fed on combined nitrogen. In contrast uninoculated plants accumulated mainly amino acids in the bleeding sap, the amount and chemical composition of which depended on the combined nitrogen source.
Similar content being viewed by others
Abbreviations
- PEP:
-
phosphoenol pyruvate
- OAA:
-
oxaloacetate
References
Bethlenfalvay, G.J., Phillips, D.A.: Interactions between symbiotic nitrogen fixation, combined-N application and photosynthesis in Pisum sativum. Physiol. Plant. 42, 119–123 (1978)
Christeller, J.T., Laing, W.A., Sutton, W.D.: Carbon dioxide fixation by lupin root nodules. I. Characterization, association with phosphoenol pyruvate carboxylase and correlation with nitrogen fixation during nodule development. Plant Physiol. 60, 47–50 (1977)
Fujihara, S., Yamamoto, K., Yamaguchi, M.: A possible role of allantoin and the influence of nodulation on its production in soybean plants. Plant Soil. 48, 233–242 (1977)
Herridge, D.F., Atkins, C.A., Pate, J.S., Rainbird, R.M.: Allantoin and allantoic acid in the nitrogen economy of the cowpea (Vigna unguiculata L. Walp). Plant Physiol. 62, 495–498 (1978)
Kamberger, W.: Regulation of symbiotic nitrogen fixation in root nodules of alfalfa (Medicago sativa) infected with Rhizobium meliloti. Arch. Microbiol. 115, 103–108 (1977)
Latimore, M., Giddens, J., Ashley, D.A.: Effect of ammonium and nitrate nitrogen upon photosynthate supply and nitrogen fixation by soybeans. Crop Sci. 17, 399–404 (1977)
Matsumoto, T., Yatazawa, M., Yamamoto, Y.: Effects of exogenous nitrogen-compounds on the concentrations of allantoin and various constituents in several organs of soybean plants. Plant Cell Physiol. 18, 613–624 (1977)
Miflin, B.J., Lea, P.J.: Amino acid metabolism. Annu. Rev. Plant Physiol. 28, 299–329 (1977)
Neves, M.C.P.: Carbon and Nitrogen Metabolism of cow Pea (Vigna unguiculata L.), pp. 229. Ph.D. Thesis, University of Reading 1978
Neyra, C.A.I., Franco, A.A., Pereira, J.C.: Seasonal patterns of nitrate reductase and nitrogenase activities in Phaseolus vulgaris. Plant. Physiol. (Suppl.) 59, 127 (1977)
Olson, J.A.: Spectrophotometric measurement of α-keto acid semicarbazones. Arch. Biochem. Biophys. 85, 225–233 (1959)
Postgate, J.: The acetylene reduction test for nitrogen fixation. In: Methods in Microbiology, vol. 6b, pp. 343, Norris, J.R., Ribbons, D.W., eds. London: Academic Press 1971
Peterson, J.B., Evans, H.J.: Properties of pyruvate kinase from soybean nodule cytosol. Plant Physiol. 61, 909–914 (1978)
Tajima, S., Yamamoto, Y.: Enzymes of purine catabolism in soybean plants. Plant Cell Physiol. 16, 271–282 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cookson, C., Hughes, H. & Coombs, J. Effects of combined nitrogen on anapleurotic carbon assimilation and bleeding sap composition in Phaseolus vulgaris L.. Planta 148, 338–345 (1980). https://doi.org/10.1007/BF00388121
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388121