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Chloroplast DNA from three archegoniates

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Abstract

1. DNA from female and male Sphaerocarpos donnellii (liverwort) plants exhibits at least two species with buoyant densities of 1.703 (main band) and 1.691 (satellite) g cm-3 in CsCl equilibrium gradients. At least part, if not all, of the satellite DNA is localized in plastids. It consists of up to 90% of uniformly sized circular molecules of an average circumference of 38.5 μm. Compared to other Chlorophyta, the liverwort's cpDNA is unusually low both in diensity and contour length. — 2. On the hand, cpDNA from the ferns Asplenium nidus and Pteris vittata resembles those of higher plants in buoyant density (1.697 g cm-3) and circumference (about 44.8 μm). — 3. Analysis of DNA from the archegoniate chloroplasts with restriction endonucleases indicates chat the cyclic molecules are monomers. — 4. The results show that the circular molecules found in cpDNA of higher plants do not represent the functionally required minimum size of DNA in plastids.

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Abbreviations

cpDNA:

chloroplast

DNA:

nucDNA=nuclear

DNA:

Sal I=restriction endonuclease from Streptomyces albus S

Eco RI:

restriction endonuclease from Escherichia coli, carrying resistance factor 1

DTT:

dithiothreitol (Cleland's reagent)

Saline-EDTA:

0.15 M NaCl, 0.1 M ethylene diamine tetraacetic acid, pH 8.0

SSC:

0.15 M NaCl, 0.015 M Na citrate, pH 7.2

DNAase:

deoxyribonuclease

Md:

Megadalton

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Author notes

  1. Harinder K. Palta

    Present address: Harding Research Laboratory, The New York Botanical Garden, 10458, Bronx, NY, USA

Authors and Affiliations

  1. Botanisches Institut der Universität, Universitätsstraße 1, D-4000, Düsseldorf, Federal Republic of Germany

    Reinhold G. Herrmann, Harinder K. Palta & Klaus V. Kowallik

Authors
  1. Reinhold G. Herrmann
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  2. Harinder K. Palta
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  3. Klaus V. Kowallik
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Dedicated to the memory of Prof. Dr. Edgar Knapp

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Herrmann, R.G., Palta, H.K. & Kowallik, K.V. Chloroplast DNA from three archegoniates. Planta 148, 319–327 (1980). https://doi.org/10.1007/BF00388118

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  • DOI: https://doi.org/10.1007/BF00388118

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