Abstract
Excretion and absorption of glycolate by young cells of Scenedesmus obliquus (Turp.) Krüger strain D3 grown synchronously with 2% CO2 was compared after no pretreatment with air (CO2-adapted) or after a 2 h adaptation to normal air (0.03% CO2) (air-adapted). At 21% O2, excretion occurred only from CO2-adapted cells at high pH (pH 8.0). Under conditions where no excretion occurred, external glycolate (0.2 mM) was taken up by both air-and CO2-adapted cells at a much faster rate at pH 5 than at pH 8. The uptake was accompanied by an apparent stoichiometric uptake of H+. CO2-adapted algae exhibited high uptake rates that were even higher in the dark than in the light. Air-adapted algae showed high uptake rates in the light but only minimal uptake in the dark. The uptake rate was decreased to about 1/3 with 5% CO2, except with CO2-adapted cells in the light, in which a slight stimulation occurred. Cl- ions inhibited glycolate uptake by air-adapted cells in the light; conversely, light-stimulated Cl- uptake of these cells was inhibited by glycolate. A hypothesis is discussed according to which the internal pH regulates the uptake and release of Cl-, HCO -3 , and glycolate.
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Abbreviations
- DCMU:
-
3′-(3,4 dichlorophenyl)-1′, 1-dimethyl urea
- FCCP:
-
carbonyl cyanide p-trifluoro-methoxyphenylhydrazone
- HEPES:
-
2-(4-(2-hydroxyethyl)-piperazinyl) ethanesulfonic acid
- HPMS:
-
α-hydroxypyridinemethanesulfonate
- MES:
-
2-morpholinoethanesulfonic acid
- PCV:
-
packed cell volume
References
Calkins, V.P.: Microdetermination of glycolic and oxalic acids. Ind. Eng. Chem. 15, 762–763 (1943)
Codd, G.A., Lord, J.M., Merrett, M.J.: The glycollate oxidizing enzyme of algae. FEBS Letters 5, 341–342 (1969)
Codd, G.A., Merrett, M.J.: The regulation of glycolate metabolism in division synchronized cultures of Euglena. Plant Physiol. 47, 640–643 (1971)
Colman, B., Miller, A.G., Grodzinski, B.: A study of the control of glycolate excretion in Chlorella. Plant Physiol. 53, 395–397 (1974)
Coombs, J., Whittingham, C.P.: The mechanism of inhibition of photosynthesis by high partial pressures of oxygen in Chlorella. Proc. Roy. Soc. Lond. B. 64, 511–520 (1966)
Döhler, G., Braun, F.: Untersuchung der Beziehung zwischen extrazellulärer Glycolsäureausscheidung und der photosynthetischen CO2-Aufnahme bei der Blaualge Anacystis nidulans. Planta (Berl.) 98, 357–361 (1971)
Findenegg, G.R.: Beziehungen zwischen Carboanhydraseaktivität und Aufnahme von HCO -3 und Cl- bei der Photosynthese von Scenedesmus obliquus. Planta (Berl.) 116, 123–131 (1974a)
Findenegg, G.R.: Carbonic anhydrase and the driving force of light-dependent uptake of Cl- and HCO -3 by Scenedesmus. In: Membrane Transport in Plants, pp. 192–196, Zimmermann, U., Dainty, J., eds. Berlin-Heidelberg-New York: Springer 1974b
Findenegg, G.R.: Correlations between accessibility of carbonic anhydrase for external substrate and regulation of photosynthetic use of CO2 and HCO -3 by Scenedesmus obliquus. Z. Pflanzenphysiol. 79, 428–437 (1976a)
Findenegg, G.R.: Estimation of bicarbonate fluxes in Scenedesmus obliquus. In: Transmembrane Ionic Exchanges in Plants, Ducet, D., Heller, R., Thellier, M., eds. Paris: Centre National de la Recherche Scientifique 1976b, in press
Findenegg, G.R.: Zur Frage eines HCO -3 /OH- Austausches im Plasmalemma von Scenedesmus obliquus. Ber. dtsch. bot. Ges. 89, 277–284 (1976c)
Han, T.W., Eley, J.H.: Glycolate excretion by Anacystis nidulans: Effect of HCO -3 concentration, oxygen concentration and light intensity. Plant & Cell Physiol. 14, 285–292 (1973)
Hess, J.L., Tolbert, N.E.: Glycolate pathway in algae. Plant Physiol. 42, 371–379 (1967)
Hess, J.L., Tolbert, N.E., Pike, L.M.: Glycolate biosynthesis by Scenedesmus and Chlorella in the presence or absence of NaHCO3. Planta (Berl.) 74, 278–285 (1967)
Ingle, R.K., Colman, B.: The relationship between carbonic anhydrase activity and glycolate excretion in the bluegreen alga Coccochloris peniocystis. Planta (Berl.) 128, 217–223 (1976)
Lord, J.M., Codd, G.A., Merrett, M.J.: The effect of light quality on glycolate formation and excretion in algae. Plant Physiol. 46, 855–856 (1970)
Merrett, M.J., Lord, J.M.: Glycolate formation and metabolism by algae. New Phytol. 72, 751–768 (1973)
Miller, R.M., Meyer, C.M., Tanner, H.A.: Glycolate excretion and uptake by Chlorella. Plant Physiol. 38, 184–188 (1963)
Nalewajko, C., Chowdhuri, N., Fogg, G.E.: Excretion of glycollic acid and the growth of a planctonic Chlorella In: Microalgae and Photosynthetic Bacteria pp. 171–183. Tokyo: University of Tokyo Press 1963
Nelson, E.B., Tolbert, N.E.: The regulation of glycolate metabolism in Chlamydomonas reinhardtii. Biochim. biophys. Acta (Amst.) 184, 263–270 (1969)
Nelson, E.B., Tolbert, N.E., Hess, J.L.: Glycolate stimulation of oxygen evolution during photosynthesis. Plant Physiol. 44, 55–59 (1969)
Orth, G.M., Tolbert, N.E., Jiminez, E.: Rate of glycolate formation during photosynthesis at high pH. Plant Physiol. 41, 143–147 (1966)
Raven, J.A., Smith, F.A.: The regulation of intracellular pH as a fundamental biological process. In: Ion Transport in Plants, pp. 271–278, Anderson, W.P., ed. London-New York: Academic Press 1973
Schou, L., Benson, A.A., Bassham, J.A., Calvin, M.: The path of carbon in photosynthesis. XI. The role of glycolic acid. Physiol. Plant. 3, 487–495 (1950)
Stabenau, H.: Über die Ausscheidung von Glycolsäure bei Chlorogonium elongatum Dangeard. Biochem. Physiol. Pflanzen 163, 43–51 (1972)
Tolbert, N.E.: Photorespiration. In: Algal Physiology and Biochemistry, pp. 474–504, Stewart, W.D.P., ed. Oxford-London-Edinburgh-Melbourne: Blackwell 1974
Tolbert, N.E., Zill, L.P.: Excretion of glycolic acid by algae during photosynthesis. J. Biol. Chem. 222, 895–906 (1956)
Vennesland, B., Jetschmann, K.: The effect of cyanide and some other carbonyl binding reagents on glycolate excretion by Chlorella vulgaris. Planta (Berl.) 128, 81–84 (1976)
Warburg, O., Krippahl, G.: Glykolsäurebildung in Chlorella. Z. Naturforsch. 15b, 197–199 (1960)
Zelitch, I.: Photosynthesis, photorespiration and plant productivity. New York: Academic Press 1971
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Findenegg, G.R. Interactions of glycolate-, HCO -3 -, Cl--, and H+-balance of Scenedesmus obliquus . Planta 135, 33–38 (1977). https://doi.org/10.1007/BF00387972
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DOI: https://doi.org/10.1007/BF00387972